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The impedance of electrode and photostimulation artifacts (short-duration and high-amplitude spikes) are still hindering the employment of silicon-based neural probe in optogenetics. A fiber-based optrode modified with a double-layer platinum black-poly (3,4ethylenedioxythiophene) PEDOT/poly (4-styrenesulfonate) PSS (Pt-PP) coating has been developed for improvement of neural recording quality and mitigation of photoelectric artifact simultaneously. The Pt-PP coating was made by layer-by-layer electrochemical deposition followed by the ultrasonication and Cyclic Voltammetry (CV) scanning to verify its mechanical and electrochemical stability. Both in-vitro and in-vivo experiments demonstrated that Pt-PP coated optrode had outstanding recording performance (high signal-to-noise ratio about 9.64) and low photoelectric amplitude (850 μV). The artifact recovery time of Pt-PP coated optrode (0.3 ms) after photostimulation was significantly decreased when compared to platinum black (6 ms) or PEDOT/PSS (0.7 ms) coated one which has potential to retain high-quality neural signals in animal experiments. At last, the optogenetics experiments revealed the capability of Pt-PP coated optrode to record the change in neural spike rate with certain spatial resolution and shorter artifact recovery time. These results suggest that Pt-PP coating has great potential for neural electrodes in the application of neuroscience. • A layer-by-layer electrochemical deposition Platinum black-PEDOT/PSS (Pt-PP) coating on microelectrode is described. • The design and in vivo application of a fiber based Michigan optrode with flexibility PI connection is verified. • The performance of recording and mitigation of photoelectric of Pt-PP modified optrode are better than Platinum Black and PEDOT/PSS modified one. • The mechanical and electrochemical stability of Pt-PP modified optrode were verified by ultrasonication and CV scanning tests. • The Pt-PP modified optrode recorded higher quality spikes with lower artifact amplitude, shorter recovery time during optogenetics experiments. [ABSTRACT FROM AUTHOR]

Abstract The brain-computer interface (BCI) devices are of prime important for study of nervous system as well as diagnosis and treatment of neurological disorders. To meet the needs of the BCI devices in high-density integration and multi-functionalization, 3-dimensional (3D) drivable optrode array with laser diodes (LDs) coupled waveguides was developed. The unique device realizes the 3D integration of the optrodes and avoids fiber tangle and tissue heating by adopting LD coupled waveguide structure. Besides, the postoperative position adjustment of the optrode array was achieved by integrating with a 3D printed micro-drive. Most importantly, high-resolution neural stimulations and recordings were achieved for study of working memory related neural circuits in four brain regions of mice including prelimbic cortex (PrL), mediodorsal thalamic nucleus (MD), dorsal medial caudate nucleus (dmCP) and posterior motor cortex 2 (pM2). The results indicate that this novel device is promising for the research of complex neural networks. Highlights • The design and in-vivo use of a novel 3D drivable optrode array was described. • Fiber tangle and tissue heating was avoided by adopting LD coupled waveguide. • Postoperative position adjustment was achieved by integrating with micro-drive. • High-resolution neural manipulation was achieved in four brain regions of mice. [ABSTRACT FROM AUTHOR]