1. Cell-derived microvesicles mediate the delivery of miR-29a/c to suppress angiogenesis in gastric carcinoma.
- Author
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Zhang, Haiyang, Bai, Ming, Deng, Ting, Liu, Rui, Wang, Xia, Qu, Yanjun, Duan, Jingjing, Zhang, Le, Ning, Tao, Ge, Shaohua, Li, Hongli, Zhou, Likun, Liu, Yuchen, Huang, Dingzhi, Ying, Guoguang, and Ba, Yi
- Subjects
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MICRORNA , *NEOVASCULARIZATION , *STOMACH cancer patients , *VESICLES (Cytology) , *CELLULAR signal transduction , *CANCER chemotherapy , *ANIMAL experimentation , *CANCER , *CELL lines , *CELL membranes , *CELL physiology , *DRUG delivery systems , *GENES , *MICE , *RNA , *STOMACH tumors , *VASCULAR endothelial growth factors , *PATHOLOGIC neovascularization - Abstract
Microvesicles (MVs) secreted from cells have been found to mediate signal transduction between cells. In the tumor microenvironment, VEGF released from cancer cells plays a key role in promoting tumor angiogenesis. In this study, we characterized the inhibitory effect of MV-delivered miR-29a/c on angiogenesis and tumor growth in gastric cancer (GC). We found that the downregulation of miR-29a/c increases VEGF expression and release in GC cells, promoting the growth of vascular cells. By simulating the tumor microenvironment, the MV-delivered miR-29a/c significantly suppresses VEGF expression in GC cells, inhibiting vascular cell growth, metastasis, and tube formation. We also used a tumor implantation mouse model to show that secreted MVs containing overexpressed miR-29a/c significantly reduced the growth rate of the vasculature and tumors in vivo. To conclude, our results contribute to a novel anti-cancer strategy using miRNA-containing MVs to control tumor cell growth by blocking angiogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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