1. c-Maf suppresses human T-cell leukemia virus type 1 Tax by competing for CREB-binding protein.
- Author
-
Hieshima K, Nagakubo D, Shigeta A, Tanaka Y, Hoshino H, Tsukasaki K, Yamada Y, and Yoshie O
- Subjects
- Blotting, Western, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes virology, CREB-Binding Protein genetics, Cell Transformation, Viral, Gene Products, env genetics, Gene Products, env metabolism, Gene Products, tax antagonists & inhibitors, Gene Products, tax genetics, HTLV-I Infections genetics, HTLV-I Infections virology, Human T-lymphotropic virus 1 genetics, Humans, Jurkat Cells, Leukemia-Lymphoma, Adult T-Cell genetics, Leukemia-Lymphoma, Adult T-Cell metabolism, Leukemia-Lymphoma, Adult T-Cell virology, Luciferases metabolism, Promoter Regions, Genetic, Proto-Oncogene Proteins c-maf antagonists & inhibitors, Proto-Oncogene Proteins c-maf genetics, RNA, Messenger genetics, RNA, Small Interfering genetics, Receptors, CCR4 genetics, Receptors, CCR4 metabolism, Retroviridae Proteins, Oncogenic genetics, Retroviridae Proteins, Oncogenic metabolism, Reverse Transcriptase Polymerase Chain Reaction, Th2 Cells, Transcriptional Activation, Virion, CREB-Binding Protein metabolism, Gene Products, tax metabolism, HTLV-I Infections metabolism, Human T-lymphotropic virus 1 metabolism, Proto-Oncogene Proteins c-maf metabolism
- Abstract
Latent infection of human T-cell leukemia virus type 1 (HTLV-1) is considered to be preferentially associated with CCR4(+) CD4(+) T cells. Here we report that c-Maf, one of the critical transcription factors for Th2 differentiation, suppresses the transcriptional activity of HTLV-1 Tax by competing for CREB-binding protein. Notably, c-maf expression is selectively induced in a fraction of CCR4(+) CD4(+) T cells upon activation. Furthermore, c-Maf significantly decreases Tax-induced HTLV-1 envelope gp46 gene expression from an infectious HTLV-1 molecular clone and tax expression in a cell-free HTLV-1 infection system. Collectively, c-Maf may play a role in latent infection of HTLV-1 in CCR4(+) CD4(+) T cells by negatively regulating Tax activity., (© 2011 Japanese Cancer Association.)
- Published
- 2011
- Full Text
- View/download PDF