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Your search keyword '"Nathalie Van Bruaene"' showing total 2 results
Start Over Author "Nathalie Van Bruaene" Journal cellular physiology and biochemistry
2 results on '"Nathalie Van Bruaene"'

1. Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Author

Jan H. Spaas, Sarah Y. Broeckx, Koen Chiers, Stephen J. Ferguson, Marco Casarosa, Nathalie Van Bruaene, Ramses Forsyth, Luc Duchateau, Alfredo Franco-Obregón, and Karin Wuertz-Kozak

Subjects
Peripheral Blood, MSCs, Cartilage, Chondrogenic, Horse, Physiology, QP1-981, Biochemistry, QD415-436
Abstract

Background: Clinical results of regenerative treatments for osteoarthritis are becoming increasingly significant. However, several questions remain unanswered concerning mesenchymal stem cell (MSC) adhesion and incorporation into cartilage. Methods: To this end, peripheral blood (PB) MSCs were chondrogenically induced and/or stimulated with pulsed electromagnetic fields (PEMFs) for a brief period of time just sufficient to prime differentiation. In an organ culture study, PKH26 labelled MSCs were added at two different cell densities (0.5 x106 vs 1.0 x106). In total, 180 explants of six horses (30 per horse) were divided into five groups: no lesion (i), lesion alone (ii), lesion with naïve MSCs (iii), lesion with chondrogenically-induced MSCs (iv) and lesion with chondrogenically-induced and PEMF-stimulated MSCs (v). Half of the explants were mechanically loaded and compared with the unloaded equivalents. Within each circumstance, six explants were histologically evaluated at different time points (day 1, 5 and 14). Results: COMP expression was selectively increased by chondrogenic induction (p = 0.0488). PEMF stimulation (1mT for 10 minutes) further augmented COL II expression over induced values (p = 0.0405). On the other hand, MSC markers remained constant over time after induction, indicating a largely predifferentiated state. In the unloaded group, MSCs adhered to the surface in 92.6% of the explants and penetrated into 40.7% of the lesions. On the other hand, physiological loading significantly reduced surface adherence (1.9%) and lesion filling (3.7%) in all the different conditions (p Conclusion: The present study demonstrates that primed chondrogenic induction of MSCs at a lower cell density without loading results in significantly enhanced and homogenous MSC adhesion and incorporation into equine cartilage.

Published
2015
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2. Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Author

Alfredo Franco-Obregón, Jan H. Spaas, Marco Casarosa, Sarah Broeckx, Stephen J. Ferguson, Luc Duchateau, Nathalie Van Bruaene, Koen Chiers, Ramses Forsyth, Karin Wuertz-Kozak, and Supporting clinical sciences

Subjects
Pathology, Physiology, MSCs, Cell Count, Osteoarthritis, Cartilage Oligomeric Matrix Protein, Horse, lcsh:Physiology, DOUBLE-BLIND, lcsh:QD415-436, Cells, Cultured, lcsh:QP1-981, biology, Cell Differentiation, DEFECTS, Chondrogenic, medicine.anatomical_structure, ANIMAL-MODELS, KNEE, medicine.symptom, Chondrogenesis, medicine.medical_specialty, Organ culture, MESENCHYMAL STEM-CELLS, lcsh:Biochemistry, Lesion, Electromagnetic Fields, Organ Culture Techniques, THICKNESS, Cell Adhesion, medicine, Animals, ELECTROMAGNETIC-FIELD THERAPY, Veterinary Sciences, Horses, Cartilage, Peripheral Blood, Cell adhesion, Collagen Type II, Cartilage oligomeric matrix protein, business.industry, Mesenchymal stem cell, Biology and Life Sciences, Mesenchymal Stem Cells, IN-VITRO, medicine.disease, Surgery, JOINT DISEASE, OSTEOARTHRITIS, biology.protein, business
Abstract

Background: Clinical results of regenerative treatments for osteoarthritis are becoming increasingly significant. However, several questions remain unanswered concerning mesenchymal stem cell (MSC) adhesion and incorporation into cartilage. Methods: To this end, peripheral blood (PB) MSCs were chondrogenically induced and/or stimulated with pulsed electromagnetic fields (PEMFs) for a brief period of time just sufficient to prime differentiation. In an organ culture study, PKH26 labelled MSCs were added at two different cell densities (0.5 x106 vs 1.0 x106). In total, 180 explants of six horses (30 per horse) were divided into five groups: no lesion (i), lesion alone (ii), lesion with naïve MSCs (iii), lesion with chondrogenically-induced MSCs (iv) and lesion with chondrogenically-induced and PEMF-stimulated MSCs (v). Half of the explants were mechanically loaded and compared with the unloaded equivalents. Within each circumstance, six explants were histologically evaluated at different time points (day 1, 5 and 14). Results: COMP expression was selectively increased by chondrogenic induction (p = 0.0488). PEMF stimulation (1mT for 10 minutes) further augmented COL II expression over induced values (p = 0.0405). On the other hand, MSC markers remained constant over time after induction, indicating a largely predifferentiated state. In the unloaded group, MSCs adhered to the surface in 92.6% of the explants and penetrated into 40.7% of the lesions. On the other hand, physiological loading significantly reduced surface adherence (1.9%) and lesion filling (3.7%) in all the different conditions (p < 0.0001). Remarkably, homogenous cell distribution was characteristic for chondrogenic induced MSCs (+/- PEMFs), whereas clump formation occurred in 39% of uninduced MSC treated cartilage explants. Finally, unloaded explants seeded with a moderately low density of MSCs exhibited greater lesion filling (p = 0.0022) and surface adherence (p = 0.0161) than explants seeded with higher densities of MSCs. In all cases, the overall amount of lesion filling decreased from day 5 to 14 (p = 0.0156). Conclusion: The present study demonstrates that primed chondrogenic induction of MSCs at a lower cell density without loading results in significantly enhanced and homogenous MSC adhesion and incorporation into equine cartilage., Cellular Physiology and Biochemistry, 37, ISSN:1015-8987, ISSN:1421-9778

Published
2015

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