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BACKGROUND: In recent years, the development of liver organoids has made it a hot spot in the field of international liver disease research, but there is still no article on the bibliometric analysis of liver organoids. OBJECTIVE: To explore the hot trends in liver organoids in the last 20 years based on bibliometrics and visualization analysis. METHODS: We searched the articles about liver organoids in the Web of Science Core Collection from January 1, 2002 to November 12, 2022. Origin, Office, and CiteSpace software were used for bibliometrics and visualization analysis. We statistically analyzed the number of annually published articles, countries, institutions, authors, journals, and keywords of the articles by generating charts. RESULTS AND CONCLUSION: The number of articles, citation frequency, institutions and personnel involved in the research about liver organoids showed an overall upward trend in the last 20 years, indicating that the field was growing rapidly and attention was increasing. The USA had published the most papers and had the strongest influence in this field. Although it had invested a lot of time and energy, the number of papers published by a single research institution in the USA was not the highest among many research institutions. China was second only to the USA in the number of publications, with the Chinese Academy of Sciences and Fudan University leading the list. Utrecht University in the Netherlands was the institution with the most publications. Clevers H was the author with the highest number of articles. The article with the highest co-citation frequency was “Long-term culture of genome-stable bipotent stem cells from adult human liver”. The main fields of study for liver organoids were Molecular Science, Biology, and Immunology. The most frequently occurring keywords were stem cell, in vitro, and culture. The research hotspots in the liver organoids field were mainly focused on in vitro stem cell three-dimensional culture, differentiation and gene expression. [ABSTRACT FROM AUTHOR]

BACKGROUND: Adolescent idiopathic scoliosis is the most common scoliosis deformity, accounting for about 80% of all scoliosis. Its pathogenesis is unknown. In recent years, the incidence rate has shown an obvious upward trend, which seriously threatens the physical and mental health of adolescents. OBJECTIVE: To perform the visualization analysis of the relevant literature on adolescent idiopathic scoliosis in recent 20 years through the Bibliometrics method, and explore the research hotspots and trends in this field to provide references for further research. METHODS: The relevant articles included in the Web of Science core collection database from January 1, 2002 to December 31, 2021 were searched by computer. The scientific knowledge map was drawn by CiteSpace 5.8.R3 software. The cooperation network, co-citation network, keyword co-occurrence, burst, clustering and timeline map were visually analyzed. RESULTS AND CONCLUSION: A total of 5 245 papers were included. In the past 20 years, the annual publication volume has shown a fluctuating upward trend. The analysis of the cooperation network shows that the author with the most publications is Qiu Yong; the institution is Nanjing University; the country is USA. The author with the highest intermediary centrality is Lowe TG; the institution is Childrens Hosp Philadelphia, and the country is Switzerland. The cocitation network analysis exhibits that the author with the highest frequency of citations is Lenke LG; the document is Weinstein SL, 2013, N Engl J Med; the journal is Spine, and the author with the highest intermediary centrality is Suk SI, and the cited document is Danielsson AJ, 2001, Eur Spine J; the cited journal is Spine. Keyword analysis displays that the research hotspots mainly focus on conservative treatment, imaging parameters, pathogenesis, and genome-wide associations. Through the visualization analysis of the literature in this research field, this study clarifies the context of the research in this field, reveals the research hotspots and trends in this field, and provides research ideas and methods for many scholars. [ABSTRACT FROM AUTHOR]

BACKGROUND: Exosomes have become one of the frontiers of biomedical research in recent years because of their promising applications in clinical diagnosis and therapy. OBJECTIVE: To reveal the basic research and technology development trend of exosomes at home and abroad, and provide suggestions for related scientific and technological research and development in China. METHODS: This paper presented a quantitative analysis of the overall research and development situation, the frontier hotspots and future development trends in this research area by means of bibliometric methods and tools. RESULTS AND CONCLUSION: The research and development hotspots in this area have entered a high growth phase with the application of new tools and methods. Many new technologies and products are facing clinical applications and markets with the development of a large number of clinical translation experiments. Comparative analysis at home and abroad reveals that China has made more remarkable achievements in basic research in this field, but there are still weaknesses in the possession of independent intellectual property rights, clinical technology translation, and regulatory system standardization and management, which need to be further strengthened in the future. [ABSTRACT FROM AUTHOR]

BACKGROUND: Gastric cancer stem cells are a hot research topic in the medical field in recent years. Gastric cancer stem cells can interfere with the occurrence, metastasis, recurrence and drug resistance of gastric cancer. In theory, gastric cancer stem cells are the most promising candidate targets for the treatment of gastric cancer in the future. Therefore, studying the action mechanism of gastric cancer stem cells can not only elucidate the occurrence and development of gastric cancer, but also help to find new effective molecular targets, and has broad application prospects. OBJECTIVE: To summarize the hot spots and development trends of gastric cancer stem cells and to provide a reference for scientific researchers in related fields based on bibliometric and visualized analysis. METHODS: CiteSpace was employed to conduct the visualized analysis of annual publications, authors, countries, journals, citations and keywords of the related papers from 2011 to 2021 in the Web of Science Core Collection. RESULTS AND CONCLUSION: A total of 2 832 papers were included for analysis after screening. The annual publications increased rapidly, and the United States and China were the top contributors. Although the total number of papers published by Chinese researchers ranked the first in the world, the papers had low centrality and citation frequency, which indicated low quality and weak academic influence. Oncotarget was the journal with the largest number of articles published, and Oncology Letters was the journal with the largest number of articles published by Chinese researchers. Gastric cancer stem cells presented the trend of interdisciplinary development. The current studies about gastric cancer stem cells mainly focused on epithelial-mesenchymal transition; effect on biological function of gastric cancer cells and self-renewal. The plasticity of gastric cancer stem cells, the relationship with fatty acid oxidation and vascular endothelial growth factor, and the regulation of non-coding RNA on gastric cancer stem cells may be the focus of current and future research. The research of gastric cancer stem cells is currently in a rapid rise stage. In recent years, the action mechanism of gastric cancer stem cells is gradually clear. The treatment of gastric cancer stem cells will be a new targeted therapy strategy for gastric cancer patients in the future. [ABSTRACT FROM AUTHOR]

BACKGROUND: Femoroacetabular impingement syndrome is one of the main causes of hip pain, and there is a lack of research in the field of rehabilitation for femoroacetabular impingement syndrome in China. OBJECTIVE: To present the research trends and research hotspots in the field of femoroacetabular impingement syndrome rehabilitation based on the Web of Science and Citespace software in the form of scientific data combined with atlas and to analyze research prospects and propose future research directions. METHODS: Keywords regarding femoroacetabular impingement syndrome rehabilitation from 2012 to 2021 were searched in the Web of Science Core Collection, and the results were bibliometrically analyzed. Citespace software was then used to conduct visually analyze countries, institutions, authors, journals, keywords and co-cited references. Finally, research trends and hotspots in the past 10 years were summarized. RESULTS AND CONCLUSION: Femoroacetabular impingement syndrome rehabilitation has gradually attracted the attention of scholars and the number of publications and citations has increased yearly, with the United States taking the lead. Chinese scholars have published fewer papers in this field and only have cooperative relations with Australian and American scholars. China’s influence in this field needs to be improved. Keyword and literature co-citation burst analyses indicate that the research trends are gait function in patients with femoroacetabular impingement syndrome, clinical efficacy of non-surgical treatment/conservative treatment/physiotherapy for femoroacetabular impingement syndrome, and the efficacy of hip arthroscopy vs. non-surgical treatment. The high-frequency co-citations, high centrality and cluster analyses indicate that the research hotspots in this field are exploring the diagnosis of pain due to femoroacetabular impingement syndrome, the short-, medium-, and long-term clinical effects of non-surgical treatment vs. hip arthroscopy, the rehabilitation effect of physiotherapy, the applicability of self-report scales, the rehabilitation scheme of femoroacetabular impingement syndrome with cartilage injury or glenoid lip injury and paying attention to their rehabilitation process, preventing femoroacetabular impingement syndrome from progressing to osteoarthritis. Based on the analysis of relevant literature in the past 10 years, this review details the future research directions in this field, including explorations on the clinical efficacy of non-surgical/surgical treatment, the middle- and long-term efficacy of non-surgical treatment vs. surgical treatment, the concurrent loss of femoroacetabular impingement syndrome, and randomized controlled trials on the prognosis and rehabilitation of femoroacetabular impingement syndrome. [ABSTRACT FROM AUTHOR]

BACKGROUND: Development of tissue engineering is of great social and economic significance. Understanding the basic research strength of China in the main subfields of tissue engineering can provide reference for the optimization of resources allocation and for the formulation of corresponding development strategies. OBJECTIVE: To evaluate the basic research strength of China in the main subfields of tissue engineering. METHODS: PubMed was searched for basic research and top basic research articles in the subfields of tissue engineering (including bone and cartilage, nerve, blood vessel, liver, skin, heart, and eye tissue engineering) in the world, the United States and China. By comparing with the global average and American, Chinese basic research strength in the main subfields of tissue engineering was evaluated with indicators including the number of papers, the growth rate of papers, and the number and proportion of top papers. RESULTS AND CONCLUSION: (1) The number of basic research papers pubIished by Chinese institutions in the subfields of bone and cartilage tissue engineering, nerve tissue engineering, vessel tissue engineering, liver tissue engineering, skin tissue engineering, cardiac tissue engineering and eye tissue engineering was 4 487, 3 551, 2 869, 1 328, 1 185, 1 088 and 689, respectively, accounting for 10%-15% of worldwide papers in corresponding subfields. The amount of papers published by Chinese institutions was nearly half of that published by institutions of the United States for the first five subfields, but only 32.1% and 37.8% of that published by institutions of the United States for the latter two subfields. (2) The annual growth rate of papers published by Chinese institutions was 19.3%, 19.5%, 18.2%, 14.6%, 21.8%, 19.6% and 19.6%, respectively, for the above seven subfrelds, all of which were obviously higher than that of the global average and the United States. (3) The number of top papers published by Chinese institutions was 36, 31, 33, 24, 15, 24 and 12, and the proportion of top papers published by Chinese institutions was 0.8%, 0.9%, 1.2%, 1.8%, 1.3%, 2.2% and 1.7%, respectively, all of which were obviously lower than that of the global average and the United States. (4) China has published relatively more papers on bone and cartilage tissue engineering, nerve tissue engineering and vessel tissue engineering, and relatively less papers on cardiac tissue engineering and eye tissue engineering. The amount of papers published by Chinese institutions has been growing rapidly in each of the seven main subfields of tissue engineering, with the speed of growing being obviously higher than that of the global average and the United States. China has published a certain amount of top papers in each of the seven subfields, while the proportion of top papers for China is obviously lower than that of the global average and the United States. [ABSTRACT FROM AUTHOR]

BACKGROUND: The development of tissue engineering is of great social and economic significance. Understanding the current status of basic research in this field can provide reference for developing corresponding development strategies. OBJECTIVE: To evaluate China's basic research current status in the field of tissue engineering. METHODS: A computer-based online search of Pubmed was performed to retrieve basic research papers and top papers in the field of tissue engineering using search terms MESH terms AND free-text word. In the context of global background, China’s ability of basic research in the field of tissue engineering was analyzed based on the number of papers, the growth of the number of papers, the number and proportion of top papers. In addition, the number of papers was used to identify the global research hotspots in the field of tissue engineering. RESULTS AND CONCLUSION: The top five countries in the field of tissue engineering were the United States, China, Japan, Germany and the United Kingdom. The total number of papers in this field published by Chinese scholars reached 17,031, second only to the United States. The compound annual growth rate of the number of papers was 19.9%, and the number of top papers was 195, ranking first and last among the top five countries, respectively. Eight Chinese institutions ranked top 30 in the number of papers in this field, with an average growth rate of 21.2%, far higher than that of all the top 30 institutions in the world (21.2% vs. 14.2, P < 0.001). However, Chinese institutions produced fewer top papers as compared to other top institutions, with the proportion of top papers significantly lower than that of the average level of the top 30 institutions (2.0% vs. 5.2%; P=0.002). There were five Chinese researchers whose total publications entered top 25 of the world. China's research in tissue engineering focuses on the areas of bone and cartilage, nerves, liver, skin, heart and eye tissue engineering, which is consistent with the global status. China's basic research in the field of tissue engineering ranks second in the world and expands rapidly in recent years. A number of academic institutions and individuals with great achievements have emerged. However, China's research quality in this field is relatively low as compared to other top institutions. It is suggested to guide researchers to pay more attention to the quality and transformation of research output, and to support the development of basic subjects such as material science to lay a good foundation for the development of tissue engineering in China. [ABSTRACT FROM AUTHOR]

BACKGROUND: It has been recognized that breast cancer stem cells play an important role in the occurrence, development, metastasis, recurrence and drug resistance of breast cancer, but the action mechanism of breast cancer stem cells in it is still unclear, and the treatment of breast cancer needs further improvement. OBJECTIVE: To explore the research status and development trend in the field of breast cancer stem cells from 2011 to 2020. METHODS: The relevant literature data in the field of breast cancer stem cell research were obtained from the Web of Science database, and the two-dimensional bar diagram of the corresponding data was drawn by Microsoft excel 2019. The co-occurrence analysis function of VOSviewer software was used to draw the co-occurrence analysis chart of keywords and its superposition with time. RESULTS AND CONCLUSION: (1) A total of 1 013 papers were included in this study. The number of papers, the H index, and the number of citations from the United States were the first in the world. The number of the literature on oncology is the largest. Sun Yat-Sen University is the largest producer of literature. WICHA MS is the author with the largest number of publications. National Natural Science Foundation of China supports the largest number of outputs. The largest number of publications is published in the ONCOTARGET. (2) The combination of chemotherapy and metformin and targeted antiangiogenic therapy are the key research contents in oncology. (3) In gene genetics, sox2, ezh2, and nanog are the key genes. (4) In the treatment of breast cancer stem cells, epithelial-mesenchymal transition is the main research content. (5) In molecular biology, cd24 and cd44 are the key biomarkers. (6) “Nuclear factor-κB” and “stemness” are the closest keywords to the present time. (7) Recombinant human receptor tyrosine kinase HRG/ErbB3 signals related to the nuclear factor-κB pathway, Pterostilbene and disulfiram are the research focus, and the epithelial/mesenchymal heterogeneity, miR34a-NOTCH1 axis and LINC00511/miR-185-3p/E2F1/ Nanog axis related to the stemness of breast cancer stem cells are the key research contents. (8) The United States has the highest link intensity with other countries in co-country analysis. (9) It is concluded that in the field of breast cancer stem cells, the United States is the most powerful country. The research direction in this field is gradually tending to the application of breast cancer stem cells to the treatment of breast cancer. The research of nuclear factor-κB and stemness may be the current research hotspots in this field. [ABSTRACT FROM AUTHOR]

BACKGROUND: Mesenchymal stem cells have the characteristics of self-renewal and multi-directional differentiation, and have great potential and application prospects in the treatment of intervertebral disc diseases. OBJECTIVE: To analyze the literature of mesenchymal stem cells in the treatment of intervertebral disc degenerative diseases using bibliometric analysis, and to understand the research trends and hotspots in this field, and to provide basis and suggestions for its follow-up development. METHODS: With the theme of mesenchymal stem cell therapy for intervertebral disc degeneration, we searched the relevant literature included in the Web of Science database from 2000 to 2019, analyzed the annual number of paper published, and scientific research funding. Citespace5.5.R2 software was used to analyze the cooperation relationship of authors, countries and research institutions, the co-citation of journals or literatures. Finally, cluster analysis, burst analysis and time zone graph analysis were performed on keywords. RESULTS AND CONCLUSION: (1) A total of 648 papers were included, with journal articles as the main type of literature. (2) Most of the funding were sponsored by China and USA (more than 50%). (3) A total of 206 authors were included, out of whom 70 authors published more than 3 papers, and the principal researchers were Daisuke Sakai (Japan) focusing on mesenchymal stem cells transplantation for intervertebral disc degeneration, Judith A Hoyland (Great Britain) focusing on regulation of myeloid cell phenotype by mesenchymal stem cells, and Makarand V Risbud (US) focusing on induction of mesenchymal stem cell differentiation. (4) 125 countries and institutions were included: China and USA were the two main countries. (5) 154 journals were included, as SPINE was the journal with the most published articles. (6) The keyword analysis showed that the research in this field can be divided into three phases: phase 1 from 2005 to 2012, mainly investigating gene expression differences, chondrogenic differentiation of mesenchymal stem cells, and extracellular matrix changes in the intervertebral disc; phase 2 from 2012 to 2017, mainly investigating mesenchymal stem cell injection therapy and changes in the microenvironment of the intervertebral disc; phase 3 from 2017 to 2019, the main study is cell therapy and disc regeneration promoted by mesenchymal stem cells. (7) The probably research direction was to extract mesenchymal stem cells and induce their differentiation to nucleus pulposus, paracrine secretion of mesenchymal stem cells on the function of disc cells and mesenchymal stem cells combined with tissue engineering technology to promote intervertebral disc regeneration. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cartilage repair has always been a great challenge for orthopedic surgeons and also a hot issue in relevant fields. Recent studies have revealed the existence of cartilage progenitor cells with stem cell characteristics in the articular cartilage, providing a new entry point for cartilage repair. OBJECTIVE: By analyzing the trend of publications, author collaboration network, institutional collaboration network, co-occurrence analysis and clustering analysis of keywords in the cartilage progenitor cells-related literature, to explore the development status, research hotspots and difficulties in this field in the past 20 years, and to provide new ideas and directions for cartilage repair-related scientific research as well as clinical work. METHODS: The core ensemble database included in Web of Science 1999-2021 was used as the literature source, and Citespace 5.8.R1 was applied to visualize and analyze relevant literature and draw relevant visualization maps. RESULTS AND CONCLUSION: A total of 829 papers were included, and the analysis results showed that: the annual publication volume was basically stable at 30-50 articles with a slight downward trend; CHUANJU LIU had the most publications and the institution with the most publications was Harvard Univ; and there were 20 high-frequency keywords that formed 12 meaningful clusters. Mesenchymal stem cells, chondrocytes, osteoblasts, bone marrow stromal cells, and stromal cells are the current research hotspots. Cartilage precursor cells are currently of little research concern, and there is still great room for future exploration. Most of the current research on cartilage precursor cells focuses on the application of tissue engineering materials combined with the cells for cartilage defect repair. Current frontier hotspots in cartilage repair research include the sources of chondrocytes and cartilage precursor cells, application of exosomes, tissue-scaffold interactions and scaffold degradation, identification and application of new stimuli, understanding structural maturation, biomechanics and function, and improving implantation, fixation and rehabilitation methods. [ABSTRACT FROM AUTHOR]

BACKGROUND: The treatment of stem cells in intervertebral disc degeneration has always been a research hotspot. It is possible to predict and analyze the research status and development trend of this field to a certain extent, applying bibliometric analysis software to perform co-occurrence and co-citation analysis of numerous literature data in this field. OBJECTIVE: To analyze research status and development trend of stem cells in the treatment of intervertebral disc degeneration by bibliometric visualization analysis. METHODS: The authors retrieved the literature on stem cells in the treatment of intervertebral disc degeneration from 1999 to 2020 through the core collection of Web of Science, and used bibliometrics methods to conduct statistical analysis on the literature data, combined with Citespace 5.6.R5 and VOSviewer 1.6.15 software to transform the visualization atlas, analyze the research status and development trend of stem cells in the treatment of intervertebral disc. RESULTS AND CONCLUSION: (1) A total of 613 articles were included. The number of publications of stem cells in intervertebral disc treatment research is increasing year by year. Among them, China and the United States rank first and second with 232 and 174 publications respectively. (2) The United States has a total citation frequency (5 522), h index (42), and betweenness centrality (0.75) ranks first, both higher than the second place in China (3 169, 28, 0.16). (3) The top ten cited references introduced the research on the treatment of intervertebral disc degeneration with microscopic mesenchymal stem cells from various aspects, and seven of them were related to nucleus pulposus like differentiation of bone marrow mesenchymal stem cells. Two papers are related to the best cell source and cell carrier generation in the treatment of intervertebral disc regeneration. One is related to the clinical trial study of the treatment of degenerative disc regeneration. (4) The timeline chart of reference co-citation shows that the most references are published in clusters of “Mesenchymal Stem Cells”, “Notochord Cells”, “End Plates”, and “Adipose derived Mesenchymal Stem Cells”. From a year of publication perspective, the clustering “notochord cells”, “notochord”, and “injection” published in the latest year. (5) Clustering keywords “intervertebral disc degeneration”, “migration”, “notochord cells”, “genipin” and “hydrogel” correspond exactly to the above. It was confirmed that the keywords suddenly appeared and found that present research hotspots are: mesenchymal stem cell nucleus pulposus-like differentiation, the best cell source in the treatment of intervertebral disc regeneration and the clinical effectiveness and biosafety of cell carrier construction and intervertebral disc regeneration treatment. (6) There are five new keywords from 2017 to 2019, namely “injection”, “intervertebral disc degeneration”, “inflammation”, “apoptosis”, and “puncture”, mainly focusing on cell apoptosis, inflammatory reaction, intervertebral disc regression, and cell transplantation methodology. (7) The above-mentioned global trend analysis suggests that as the amount of stem cells published in the treatment of intervertebral disc degeneration continues to increase, related research is also constantly deepening. The current research focuses on the nucleus pulposus-like differentiation of mesenchymal stem cells, the best cell source and cell carrier integration in the treatment of intervertebral disc regeneration, and the clinical effectiveness and biological safety of integration of intervertebral disc regeneration treatment. In-depth exploration of the mechanism of intervertebral disc degeneration and inflammatory response and improvement of cell transplantation methodology are the focus of future research. [ABSTRACT FROM AUTHOR]

BACKGROUND: Functional near-infrared spectroscopy (fNIRS) is a new non-invasive monitoring method for brain activities. fNIRS shows unique advantages for brain imaging and belongs to a new research hotspot in the field of brain science. OBJECTIVE: Based on CiteSpace knowledge map, to conduct metrology, co-occurrence analysis, co-citation analysis, cluster analysis of related international research, in order to explore the progress and hotspots of fNIRS research in the past 10 years, hoping to provide some guidance for future research. METHOD: The Web of Science was searched for relevant literature included in the Science Citation Index Expanded from 2010 to 2020. Authors, countries, institutions and keywords were taken as nodes for co-occurrence analysis using CiteSpace software. Cited journals and references were used for co-citation analysis, and keywords were clustered and burst-analyzed. RESULTS AND CONCLUSION: A total of 1 734 papers were included. Fallgatter AJ was the representative of the high yield authors. The high yield countries were the United States, Japan and China. The high yield institutions were Eberhard Karls Universität Tübingen, Drexel University and University College London. From the perspective of psychology and cognitive neuroscience, it is predicted that fNIRS research on the various mechanisms of different brain regions, especially the frontal cortex, fNIRS research on the detection of individual differences in infants and children, and research on fNIRS combined with other brain functional imaging techniques are still hot topics in the future. CiteSpace-based visual analysis of fNIRS literature included in the Web of Science in the past 10 years indicates a rapid development of fNIRS technique. This study directly reveals the authors and institutions that can cooperate, and predicts the research focus of fNIRS, which provide references for future topic selection and research direction of fNIRS research. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cartilage repair has always been a great challenge for orthopedics doctors all over the world, and it has always been a research hot-spot. OBJECTIVE: To explore the development status, research hotspots and dynamic frontiers in cartilage repair in the past 15 years based on the bibliometric, co-occurrence, co-citation and visual analysis of relevant literature. METHODS: Based on the core collection of related documents in the Web of Science (WOS) database from January 1, 2006 to April 6, 2020, CiteSpace software was used for a literature visual analysis. RESULTS AND CONCLUSION: A total of 1 632 papers were included. The results of co-occurrence analysis, burst analysis and cluster analysis show that cartilage repair belongs to the field of regenerative medicine, and the types of stem cells mainly involved in cell transplantation include human bone marrow mesenchymal stem cells, mesenchymal stem cells, artificial multi-functional stem cells, adipose tissue derived stem cells. It can be seen that stem cells are the research focus in the past, present or future. Treatments for cartilage repair include autograft chondrocyte transplantation, mosaic plasty, tissue-engineered cartilage implantation, intraarticular administration, combined gene transfer, which are widely used at present. Factors influencing gene expression include overexpressed human fibroblast growth factor, expanded chondrocytes, sulfur oxide 9, recombinant adeno-associated virus, γ- glutamic acid, cetylpyridinium chloride, mechanical properties, and telomerase activity. These biocompatible materials and factors of culture medium can guide gene carriers in cartilage repair, which is a hot topic in the future. Tissue engineering technology such as polycaprolactone membrane, polyglycolic acid keratin hydrogel and other tissue engineering materials used for mesenchymal stem cell transplantation is the current research trend. The above results show that through the application of CiteSpace software for bibliometric and visual analysis, we can intuitively see the development of cartilage repair in the past 15 years, mainly revealing that stem cell therapy for cartilage defects, different biocompatible materials and different gene transfer technologies for cartilage repair are the current research hots and the developmental trends of cartilage repair, providing different ideas and research directions of animal experiments and clinical trials for domestic scientific research and clinical workers. [ABSTRACT FROM AUTHOR]

BACKGROUND: In recent years, three-dimensional (3D) printing technology is widely used in various fields, and its application in the medical field is also increasing, which provides a new direction for the development of medical research. OBJECTIVE: To analyze the literature of 3D printing in biomedical field in recent 5 years, and illustrate the current research status, hot spots and development trends by using knowledge mapping analysis. METHODS: PubMed database was retrieved for related literature on 3D printing in biomedical field published from 2015 to 2020, and the citation relationship between articles was obtained from the core collection of web of science by PMID. The tool of bibliometrics and VOSviewer were used to analyze the author, organization, country and key words, and draw knowledge mapping. RESULTS AND CONCLUSION: (1) A total of 3 471 articles were included. A trend toward an increasing number of publications in recent 5 years was evident. According to the index trend line, the number of papers will keep growing. In the future, the research of 3D printing in biomedical field will continue to attract the attention of researchers. (2) Among the 22 core journals identified, the percent of journals with JCR Q ≥ 2 was 81.8%, and the quality of journals was high. (3) According to the keyword co-occurrence clustering results, the current research was divided into four categories: clinical application, tissue engineering scaffold, application in the field of pharmaceutical preparations, and 3D bioprinting. (4) VOSviewer knowledge mapping analysis showed that the selection of biomedical 3D printing tissue scaffold materials and scaffold microstructure design were the focus and hotspot in recent years and the basis of expanding clinical application of tissue engineering. (5) It was a trend from single to multiple composite materials in scaffold materials. The 3D microstructure of scaffolds, including porosity, pore size and interconnected pore structure, played a key role in enhancing cell viability and promoting tissue growth. (6) The preparation of bioink with cell activity was the focus of 3D bioprinting research, including the properties of biomaterial scaffolds, such as hydrogel, alginate and extracellular matrix, cross-linking and stiffness of scaffolds, and stem cell printing. (7) In the application of 3D printing pharmaceutical preparations, the personalized setting of drug delivery system is realized through the hotspot research, such as drug preparation printing technology, polymer, nanoparticles, and microfluidic chip. [ABSTRACT FROM AUTHOR]

BACKGROUND: Platelet-rich plasma is a platelet concentrate obtained from autologous whole blood after centrifugation, which contains a lot of growth factors and proteins. Activated platelets can release a variety of growth factors so as to accelerate cell proliferation and differentiation, and can effectively promote tissue repair. Platelet-rich plasma has been widely used in various clinical fields because of its convenient sampling and simple preparation. Overseas research on platelet-rich plasma has been carried out for a long time, and has achieved certain results. OBJECTIVE: To review the research advance of platelet-rich plasma at abroad in recent 5 years. METHODS: PubMed database was retrieved with the keywords of “plasma, platelet-rich, platelet rich plasma, platelet-rich plasma’’ and the retrieval time was from 2014-2018. The visual tool VOSviewer was used to deeply search the title information of the papers retrieved. The annual and national published number, and research topics were discussed. The knowledge map generated by software is used to visually display the hotspot of platelet-rich plasma research at abroad. RESULTS AND CONCLUSION: Thirty-eight relevant articles were retrieved. The results show that in the past five years the number of papers published on platelet-rich plasma in foreign countries has increased year by year, and the United States has the largest number of publications. Through co-word clustering analysis, the hotspots of platelet-rich plasma are mainly concentrated on the following five aspects: animal experiments, orthopedics, cytology experiments, stomatology and chronic wounds. Research results provide reference for the direction and hotspot of research on platelet-rich plasma in China. [ABSTRACT FROM AUTHOR]

BACKGROUND: Mechanisms undelying diagnosis and treatment of arthritis can be analyzed by metabonomics to study the metabolites. The combination of metabonomics and bibliometrics can systematically clarify the research status of osteoarthritis. OBJECTIVE: To analyze and summarize the research status of metabonimics in arthritis, and to prospect the future tendency. METHODS: CNKI, VIP, WanFang, CBM, PubMed, EBSCO, Web of Science and Elsevier databases were searched for the articles addressing the metabonimics in arthritis published before May 2017. The keywords were “metabolomics and arthritis” in English and Chinese, respectively. Initially 201 articles were retrieved, and finally 59 articles were included based on the inclusion and exclusion criteria for basic information and result analysis. RESULTS AND CONCLUSION: (1) Literature of metabonomics on arthritis began to be reported from 2007, and the number of literature increased with time. (2) The first author’s affiliations were concentrated in universities 37(63%), hospitals 15 (25%) and institutes 7 (12%). (3) The articles included 44 articles from journals (75%), 12 dissertation (19%), 4 conference papers (7%), and the 44 papers were published in 38 kinds of journals. (4) Totally 36 articles were funded, 29 articles (49%) funded by the National Natural Science Foundation, 18 (31%) funded by department-level foundation, 10 (17%) funded by provincial foundation, 5 (8%) foreign foundation and 5 (8%) funded by school foundation. (5) The types of arthritis were mainly rheumatoid arthritis 40 (68%), osteoarthritis 7 (12%), gouty arthritis 6 (10%) and others 6 (10%). (6) The main research directions were metabonomis on treatment effectiveness 30 (51%), pathogenesis of arthritis 17 (29%), Chinese medicine syndromes 6 (10%) and research progress 6 (10%). (7) Metabolomics samples in the literature included the body fluid samples 53 (90%) and tissue samples 6 (10%). (8) Metabonomics analysis techniques included liquid chromatography-mass spectrometry 33 (56%), nuclear magnetic resonance technology 15 (25%), gas chromatography-mass spectrometry 10 (17%), NMR combined with GC-MS 1 (2%). In summary, metabonomics has been extensively applied in arthritis and has been an issue of concern. Understanding the side events in Chinese medicines for arthritis based on metabonomics can provide reference for the following prospective study and clinical application. [ABSTRACT FROM AUTHOR]

BACKGROUND: With the rapid development of digital orthopaedics, navigational template has been widely used in the field of orthopaedics, but the research status of the technique in China based on bibliometric analysis is never reported. OBJECTIVE: To investigate the application status of navigational template in the orthopaedic field and to explore its tendency and hot spot based on bibliometric analysis, thereby providing reference for the development of digital orthopaedics in China. METHODS: A computer-based retrieval of CBMdisc, CNKI, CSPD, CMCI databases was conducted for articles published before December 1, 2016 using the keywords of “navigational template” and subject heading “orthopaedics” and the core orthopaedic journals published in 2016 were searched by manual. The articles were screened according to inclusion and exclusion criteria, and finally were analyzed by bibilometric study. RESULTS AND CONCLUSION: (1) In China, the first paper reporting navigational template was issued in 2008. A total of 199 articles were published in the past 9 years, with more than 10 papers yearly from 2009 to 2014, while there was a rapid increase tendency during 2015 and 2016. (2) The articles were mainly published in the orthopaedic journals and some professional journals closely related to digital orthopaedics, such as biomedical engineering, basic medicine and digital medicine. About 86.9% papers belong to Chinese core journals, including Chinese Journal of Orthopaedic Trauma, Chinese Journal of Orthopaedics, and Chinese Journal of Tissue Engineering Research. (3) There is a 99.5% cooperation rate and 6.15 degree of cooperation. Zhang Yuan-zhi, Ding Huan-wen, Lu Sheng, Chen Yu-bing, Hu Yong, Huang Xuan and Zheng Peng-fei are most productive authors, and core authors contribute to 53.8%, indicating that the group of core authors is being formed. First authors are all from hospitals and universities, mostly from Kunming General Hospital of Chengdu Military Command of PLA, General Hospital of Guangzhou Military Command of PLA, the Affiliated Hospital of Inner Mongolia Medical University and South China University of Technology. The cooperation rate of more than two institutes occupies 54.8%, indicating that the study holds a promising future with the strong support of research institutes in universities. About 59.3% articles are sponsored by funding, the national and provincial foundations accounting for 64.0% in 239 projects, and 57.6% articles were supported by more than two foundations, revealing the high research levels and academic values of this technique. (4) The articles are cited by 763 times; Lu Sheng, Chen Yu-bing, and Zhang Yuan-zhi are the highly cited authors; Kunming General Hospital of Chengdu Military Command of PLA, General Hospital of Guangzhou Military Command of PLA and Nanfang Hospital of Southern Medical University are the highly cited institutes, and Chinese Journal of Orthopaedic Trauma is the highly cited journal. (5) To conclude, based on product design, manufacture and cadaver studies of the navigational template, it has been extensively applied in the spine surgery (especially cervical surgery), orthopaedic traumatology, bone and joint surgery, bone tumor and pediatric orthopaedics, and has obtained satisfactory clinical outcomes in assisting screw placement, osteotomy and precise resection and functional reconstruction. However, there is still a lack of a large-sample, multicenter, and long-term randomized trial. [ABSTRACT FROM AUTHOR]