1. Structural analysis of the promoter of tomato I -aminocyclopropane-I -carboxylate synthase 6 gene (Le-ACS6).
- Author
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Lin Jingyu, Fan Rong, Wan Xiaorong, Charng Yee-Yung, and Wang Ningning
- Subjects
ETHYLENE ,STRUCTURAL analysis (Science) ,TOMATOES ,PLANT growth ,BIOSYNTHESIS - Abstract
Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato, Le-ACS6, a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening, is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control, we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase (LUC) reporter gene driven by a 5'-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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