1. MicroRNA-222 promotes the proliferation and migration of cervical cancer cells
- Author
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Yingyi Wang, Jiajing Cheng, Qingying Wang, Bo Zhang, Yi Wu, Fing Xing, Jin Qiu, and Yi Sun
- Subjects
Migration Assay ,biology ,medicine.diagnostic_test ,Uterine Cervical Neoplasms ,General Medicine ,Flow Cytometry ,medicine.disease_cause ,biology.organism_classification ,Molecular biology ,Flow cytometry ,HeLa ,MicroRNAs ,Microscopy, Fluorescence ,Western blot ,Cell Movement ,microRNA ,biology.protein ,medicine ,Humans ,PTEN ,Tensin ,Female ,Carcinogenesis ,Cell Proliferation ,HeLa Cells - Abstract
Purpose: This study aimed to investigate the role of small non-coding RNA-222 (microRNA-222; miR-222) in the development of cervical cancer (CC). Methods: Normal and CC specimens were obtained from 18 patients. HeLa and SiHa cells were grown in Dulbecco’s modified Eagle’s medium. RT–PCR, Western blot, migration assay, flow cytometry and immunofluorescence microscopy were used for analyses. Results: When compared with normal cervical tissues, miR-222 was upregulated in human CC, and the extent of up-regulation was associated with the extent and depth of CC invasion. Expression of miR-222 was inversely related to the expression of phosphatase and tensin homolog (PTEN) and p27. The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells. The expression of proteins (E-cadherin and paxillin) related to the proliferation and migration was also elevated. Conclusion: MiR-222 plays an important role in the tumorigenesis of CC, possibly by specifically down-regulating p27Kip1 and PTEN. Our findings suggest that miR-222 may serve as a new therapeutic target in CC.
- Published
- 2014