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Start Over Topic antioxidants Journal clinical and experimental immunology
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1. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis.

Author

Hausmann M, Obermeier F, Paper DH, Balan K, Dunger N, Menzel K, Falk W, Schoelmerich J, Herfarth H, and Rogler G

Subjects
Acute Disease, Animals, Chronic Disease, Colitis chemically induced, Colitis immunology, Colitis pathology, Colon immunology, Cytokines metabolism, Dextran Sulfate, Enzyme-Linked Immunosorbent Assay methods, Female, Inflammation Mediators metabolism, Intestinal Mucosa immunology, Lymph Nodes immunology, Macrophages immunology, Mice, Mice, Inbred BALB C, Peroxidase metabolism, Respiratory Burst drug effects, Weight Loss drug effects, Antioxidants therapeutic use, Colitis drug therapy, Glucosides therapeutic use, Phenols therapeutic use
Abstract

Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 microg/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1beta, IL-6, IL-12 tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma levels in supernatants were analysed by the beadlyte cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3.2 with acteoside versus 5.2 with phosphate-buffered saline (PBS) (P < 0.02). In chronic colitis both 120 microg (3.3 versus 5.2) or 600 microg acteoside (3.0 versus 5.2) significantly ameliorated colitis (both P < 0.02). Stimulated MLN from mice with chronic DSS-induced colitis treated with acteoside showed a significant down-regulation of IFN-gamma secretion (195 pg/ml with 600 microg acteoside versus 612 pg/ml with PBS, P < 0.02). Inhibition of oxidative burst activity with acteoside reduced mucosal tissue damage in DSS colitis and could be a therapeutic alternative for IBD treatment. Further studies of this agent are warranted.

Published
2007
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2. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis

Author

D. H. Paper, Juergen Schoelmerich, Florian Obermeier, Nadja Dunger, K. Menzel, Werner Falk, Gerhard Rogler, Martin Hausmann, K. Balan, and Hans H Herfarth

Subjects
Necrosis, Colon, medicine.medical_treatment, Immunology, Enzyme-Linked Immunosorbent Assay, Inflammatory bowel disease, Antioxidants, Mice, Glucosides, Phenols, Weight Loss, medicine, Animals, Immunology and Allergy, Mesenteric lymph nodes, Intestinal Mucosa, Colitis, Acute colitis, Peroxidase, Respiratory Burst, Mice, Inbred BALB C, business.industry, Macrophages, Dextran Sulfate, Interleukin, medicine.disease, Respiratory burst, Cytokine, medicine.anatomical_structure, Acute Disease, Chronic Disease, Animal Studies, Cytokines, Female, Lymph Nodes, Inflammation Mediators, medicine.symptom, business
Abstract

Summary Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 µg/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1β, IL-6, IL-12 tumour necrosis factor (TNF)-α and interferon (IFN)-γ levels in supernatants were analysed by the beadlyte® cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3·2 with acteoside versus 5·2 with phosphate-buffered saline (PBS) (P

Published
2007