1. Halorubrum glutamatedens sp. nov., a Halophilic Archaeon Isolated from a Rock Salt.
- Author
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Xu, Yao, Lv, Jinting, Xie, Chizhen, Sun, Siqi, Ke, Lixia, and Chen, Shaoxing
- Subjects
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ROCK salt , *SALT mining , *RIBOSOMAL RNA , *GENES , *PHOSPHOLIPIDS , *GLYCOLIPIDS - Abstract
An extremely halophilic archaeon, strain ZY8T, was isolated from a rock salt of Yunnan salt mine. It was able to grow at 12-30% (w/v) NaCl (optimum, 15-20%), pH 7.0-9.0 (optimum, pH 8.5), and 20-45 °C (optimum, 42 °C). Sequence similarity search of its 16S rRNA gene showed that strain ZY8T belonged to the genus Halorubrum, and it is closely related to species of H. aethiopicum SAH-A6T (98.6%), H. aquaticum EN-2T (98.6%), and H. halodurans Cb34T (98.5%), respectively. Strain ZY8T contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as its major phospholipids, and a sulfated diglycosyl diether as its major glycolipid. The DNA G+C content was 66.7 mol%. DNA-DNA relatedness between strains ZY8T and closely related species were far below 70%. Based on the phenotypic and phylogenetic analyses, it is proposed that strain ZY8T represents a novel species of the genus Halorubrum, for which the name Halorubrum glutamatedens sp. nov. is proposed. The type strain is ZY8T (=CGMCC 1.16026T=NBRC 112866T). [ABSTRACT FROM AUTHOR]
- Published
- 2019
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