1. Mannosylated solid lipid nanoparticles for lung-targeted delivery of Paclitaxel.
- Author
-
Sahu, Piyush Kumar, Mishra, Deepak Kumar, Jain, Nivrati, Rajoriya, Vaibhav, and Jain, Ashish Kumar
- Subjects
NANOMEDICINE ,TARGETED drug delivery ,LUNG cancer treatment ,DRUG delivery systems ,PACLITAXEL ,LIGANDS (Biochemistry) - Abstract
Objective: The present study discusses paclitaxel (PTX)-loaded mannosylated-DSPE (Distearoyl-phosphatidyl-ethanolamine) solid lipid nanoparticles (M-SLNs) using mannose as a lectin receptor ligand conjugate for lung cancer targeting and to increase the anticancer activity of PTX against A549 lung's epithelial cancer cells. Materials and methods: The PTX-SLNs were prepared by solvent injection method and mannose was conjugated to the free amine group of stearylamine. The M-SLNs obtained were characterized for their particle size, polydispersity index, zeta potential and morphology by transmission electron microscope. Results: The M-SLNs were spherical in shape with 254 ± 2.3 nm average size, positive zeta potential (3.27 mV), 79.4 ± 1.6 drug entrapment efficiency and showed the lower extent of drug release 40% over 48 h in vitro. Cytotoxicity study on A549 cell lines and biodistrubtion study of drug revealed that M-SLNs deliver a higher concentration of PTX as compared to PTX-SLNs in an alveolar cell site. Discussion and conclusion: These results suggested that mannosylated M-SLNs are safe and potential vector for lung cancer targeting. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF