1. Visualizing ClO− fluxes during homocysteine stress based on a nanoprobe.
- Author
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Tang, Jun, Li, Ziyi, Li, Sheng, Yang, Lifang, Zhu, Li, Dang, Tan, Chen, Gairong, and Ye, Yong
- Subjects
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CYSTEINE , *REACTIVE oxygen species , *HOMOCYSTEINE , *HYPOCHLORITES - Abstract
As one of important indicators of atherosclerosis (AS), excess homocysteine (Hcy), could cause oxidation stress, thus leading to inflammation and tissue damage. Hypochlorous acid/hypochlorite (HOCl/OCl−), as an important reactive oxygen species, is speculated to be an indicator of AS caused by Hcy-inducated. Here, a fluorescence nanoprobe LR NPs based on the assembly of pluronic F-127 and probe TR was developed for ClO− detection and imaging during Hcy stress. As expected, LR NPs showed good selectivity and sensitivity for ClO− detection. LR NPs can visualize the fluxes of HClO/ClO− during the stimulation of Hcy induce AS in real time, which demonstrated HClO/ClO− could be performed as an indicator of AS. [Display omitted] • A nano-fluorescent probe (LR NPs) with high sensitivity and selectivity to ClO− was designed and synthesized. • LR NPs was applied to detect endogenous and exogenous ClO− in living cells. • The fluorescent imaging of ClO− caused by Hcy-induced was reported for the first time. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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