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Exploring the connection between blood viscosity and liver in it have great significance for the detection and treatment of hyperlipidemic. Therefore, we designed a new near-infrared viscosity sensitive fluorescent probe Vis-LW based on the twisted intramolecular charge transfer mechanism. The probe exhibited a strong dependence on viscosity as well as good anti-interference performance. With the aid of Vis-LW , we successfully detected the viscosity changes in chloroquine induced cells and realized the diagnosis of diseases in hyperlipidemic mice by utilizing blood viscosity as a diagnostic indicator for hyperlipidemia. Not only that, the increased liver viscosity of hyperlipidemic mice was found for the first time, the reduced blood and liver viscosity after treatment with lipid-lowering drug fenofibrate was also tracked. This work provides a new method for the diagnosis of hyperlipidemia and offered a new idea on the research and treatment of hyperlipidemia in the future. [Display omitted] • A near infrared emission fluorescent probe for hyperlipidemia diagnosis. • The advantageous features of the probe Vis-LW include high sensitivity, good selectivity and large stokes shift. • The viscosity changes in liver organs from the hyperlipidemia and normal mice have visualized for the first time. • The change trend of the liver viscosity in the therapeutic effects of fenofibrate was explored. • This probe Vis-LW will be a powerful tool to be potentially applied in hyperlipidemia diagnosis in the case of treatment. [ABSTRACT FROM AUTHOR]

Monitoring of the viscosity levels in cells and tissues is of great significance for the preliminary diagnosis related to abnormal viscosity. However, tracking of the viscosity level of tumors in different growth stages at the tissue level has not been reported. Therefore, a new viscosity-sensitive fluorescent probe (TA-I) with large Stokes shift and high viscosity dependence is in this work designed to visualize the viscosity level of tumors at different growth stages. And this probe exhibited a 20-fold fluorescence enhancement in viscous medium. With the aid of TA-I , a rapidly increasing fluorescent signal (16 times) was captured in the cells stimulated by exogenous stimuli, and the probe was also successfully applied to monitor viscosity changes in inflammatory mice. In addition, the viscosity levels in tumor tissues of mice at different growth stages (3 days, 6 days and 10 days) were also visualized in tissue level and revealed that the intratumoral viscosity level is highly dependent on tumor growth period. Importantly, the decreased viscosity of drugs-treated tumor tissue was obtained by the probe. It is worth noting that this is the first time to visualize the viscosity change rule of tumor at different growth stages at the tissue level. Image 1 • A new fluorescent sensor for potential tumor diagnosis. • Large stokes shift, high sensitivity, excellent photo-stability and desirable biocompatibility. • Visualizing at tissues level the viscosity changes of tumors in different growth stages. • Firstly tracking the viscosity changes from drugs-treated tumor mice at tissues level. [ABSTRACT FROM AUTHOR]

Fluorescent labeling technology has been prevailing in the field of biotechnology, but in the more complex pathology, such as diabetes, tracking lipid droplets (LDs) viscosity remains a vacancy. To fill this gap, a new fluorescent probe (ABI-V), was herein synthesized for detecting the viscosity change of LDs in complicated biological systems. This probe displayed a larger stokes shift (195 nm), near-infrared emission and high sensitivity and selectivity to viscosity, exhibiting a 95-fold fluorescence enhancement from methanol to glycerol. In light of its distinguishable optical signals toward viscosity, the blood viscosity of diabetic mice was measured and a stronger fluorescence was obtained in the blood of diabetic mice with 2.5 times higher than normal mice. Importantly, with the assistance of ABI-V , the LDs viscosity changes in diabetic liver injury was successfully monitored at the organ and tissue levels and the results indicate that the viscosity of LDs in the liver increased under diabetic pathological conditions. Moreover, the LDs viscosity in metformin-treated diabetic liver injury was also detected accompanied by decreased fluorescence intensity comparing with the pure diabetic model, suggesting that the proposed probe ABI-V can achieve tracking the efficacy of diabetes treatment by monitoring the viscosity changes. This work provides a rewarding perspective to elucidate the role of LDs viscosity in the pathology of diabetes. Image 1 [ABSTRACT FROM AUTHOR]