1. The β-adrenoceptor agonist isoproterenol promotes the activity of respiratory chain complex I and lowers cellular reactive oxygen species in fibroblasts and heart myoblasts.
- Author
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De Rasmo D, Gattoni G, Papa F, Santeramo A, Pacelli C, Cocco T, Micelli L, Sardaro N, Larizza M, Scivetti M, Milano S, and Signorile A
- Subjects
- Cells, Cultured, Cyclic AMP metabolism, Fibroblasts cytology, Fibroblasts metabolism, Humans, Infant, Newborn, Mitochondria metabolism, Myoblasts, Cardiac cytology, Myoblasts, Cardiac metabolism, Phosphorylation, Adrenergic beta-Agonists pharmacology, Electron Transport Complex I metabolism, Fibroblasts drug effects, Isoproterenol pharmacology, Myoblasts, Cardiac drug effects, Reactive Oxygen Species metabolism
- Abstract
A study is presented on the effect of the β-adrenoceptor agonist isoproterenol on mitochondrial oxygen metabolism in fibroblast and heart myoblast cultures. Isoproterenol treatment of serum-limited fibroblasts and proliferating myoblasts results in the promotion of mitochondrial complex I activity and decrease of the cellular level of reactive oxygen species. These effects of isoproterenol are associated with cAMP-dependent phosphorylation of complex I subunit(s). Addition of okadaic acid, inhibitor of protein phosphatase(s), reverses the decline of complex I activity in serum-limited fibroblast cultures and activates the complex in proliferating myoblast cultures. The effects of isoproterenol on complex I activity and reactive oxygen species balance can contribute to the therapeutic effect of the drug., (2010 Elsevier B.V. All rights reserved.)
- Published
- 2011
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