1. BM61 of Bombyx mori nucleopolyhedrovirus: its involvement in the egress of nucleocapsids from the nucleus.
- Author
-
Shen H and Chen K
- Subjects
- Animals, Cell Line, Cell Nucleus metabolism, Cell Nucleus ultrastructure, Cytoplasm metabolism, Cytoplasm ultrastructure, Gene Deletion, Gene Knockout Techniques, Genes, Reporter, Microscopy, Electron, Transmission, Nuclear Envelope metabolism, Nuclear Envelope ultrastructure, Nuclear Envelope virology, Nucleocapsid ultrastructure, Nucleopolyhedroviruses genetics, Nucleopolyhedroviruses growth & development, Nucleopolyhedroviruses ultrastructure, Occlusion Body Matrix Proteins, Promoter Regions, Genetic, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction, Recombinant Fusion Proteins metabolism, Recombinant Proteins metabolism, Salivary Glands metabolism, Salivary Glands ultrastructure, Salivary Glands virology, Viral Proteins genetics, Viral Structural Proteins genetics, Viral Structural Proteins metabolism, Bombyx virology, Cell Nucleus virology, Cytoplasm virology, Nucleocapsid metabolism, Nucleopolyhedroviruses metabolism, Viral Proteins metabolism
- Abstract
All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm., (Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2012
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