1. Transcription factor Nrf1 is negatively regulated by its O-GlcNAcylation status.
- Author
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Chen, Jiayu, Liu, Xiping, Lü, Fenglin, Liu, Xinping, Ru, Yi, Ren, Yonggang, Yao, Libo, and Zhang, Yiguo
- Subjects
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TRANSCRIPTION factors , *GLUCOSAMINE , *PROTEIN expression , *IMMUNOPRECIPITATION , *SMALL interfering RNA - Abstract
O -Linked N -acetylglucosamine transferase (OGT) was identified as an Nrf1-interacting protein. Herein, we show that Nrf1 enables interaction with OGT and their co-immunoprecipitates are O -GlcNAcylated by the enzyme. The putative O -GlcNAcylation negatively regulates Nrf1/TCF11 to reduce both its protein stability and transactivation activity of target gene expression. The turnover of Nrf1 is enhanced upon overexpression of OGT, which promotes ubiquitination of the CNC-bZIP protein. Furthermore, the serine/theorine-rich sequence of PEST2 degron within Nrf1 is identified to be involved in the protein O -GlcNAcylation by OGT. Overall, Nrf1 is negatively regulated by its O -GlcNAcylation status that depends on the glucose concentrations. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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