1. miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a
- Author
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Xing Yanjiang, Han Zhengbin, He Hongjuan, Wu Qiong, Gu Ning, Liu Qi, Huang Zhijun, and Zeng Tiebo
- Subjects
Time Factors ,Molecular Sequence Data ,Biophysics ,Morphogenesis ,Spatiotemporal expression ,Dnmt3a ,Biology ,Biochemistry ,DNA Methyltransferase 3A ,Mice ,miR-370 ,Western blot ,Structural Biology ,Genes, Reporter ,microRNA ,Adrenal Glands ,Genetics ,medicine ,Animals ,DNA (Cytosine-5-)-Methyltransferases ,Luciferases ,Molecular Biology ,Gene ,Regulation of gene expression ,Mice, Inbred ICR ,medicine.diagnostic_test ,Base Sequence ,Embryogenesis ,Brain ,Gene Expression Regulation, Developmental ,Embryo ,Cell Biology ,Embryo, Mammalian ,Molecular biology ,MicroRNAs ,Organ Specificity ,Embryonic development ,Female ,Signal transduction ,Signal Transduction - Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that participate in a large variety of biological processes. In this paper, the spatiotemporal expression pattern of miR-370 was characterized during mouse embryonic development, and was found to be stage- and tissue-specifically expressed. In addition, through luciferase reporter assays and western blot analyses, DNA methyltransferase 3A (Dnmt3a) was identified as a directly regulated target of miR-370. Altogether, our results indicate that miR-370 may play important roles in the morphogenesis of diverse organs, especially brain and adrenal glands, by mediating Dnmt3a expression during mouse development.
- Published
- 2012