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The aim of the experiment was to study the effect of curcumin on the lipid metabolism of grass goldfish and find out the appropriate amount of curcumin. 360 grass goldfish with an initial weight of (40.12±1.23) g and initial body length of (10.34±0.08) cm were selected. They were randomly divided into six groups, with three replicates in each group, and each replicate consisting of 20 individuals. Different concentrations of curcumin (0, 40, 80, 160, 320, and 640 μmol/L) were injected, and the fish were cultured for 14 days. After 7 and 14 days of curcumin injection, the concentrations of serum TC and TG in the 160-640 μmol/L curcumin group were significantly lower than those in the control group ( P<0.05). The activities of AST and ALT in the hepatopancreas of grass goldfish in the 320 μmol/L curcumin group and 640 μmol/L curcumin group were significantly lower than those in the control group ( P<0.05). The activities of CAT and T-SOD in the 320 μmol/L curcumin group were significantly higher than those in the control group ( P<0.05). The study indicates that curcumin injection can reduce the concentrations of serum TC and TG to a certain extent, and has a certain repair effect on the hepatopancreas of grass goldfish, and can improve the antioxidant capacity of the hepatopancreas. [ABSTRACT FROM AUTHOR]

The experiment aimed to study the effect of Bupleuri radix and its extract on the growth, physiology and biochemistry, liver antioxidant, and antibacterial ability of juvenile koi carp. A total of 360 juvenile koi carp with an initial body weight of (21.0±0.5) g were randomly divided into three groups with three replicates in each group and 40 juvenile koi carp in each replicate. The juvenile koi carp in the group K, group C, and group T were fed a basal diet, a basal diet + 5‰ Bupleuri radix meal, a basal diet + 5‰ Bupleuri radix extract. The experiment lasted for 14 days. The results showed that in the serum of juvenile koi carp, the activity of lysozyme (LZM) in the group C and alkaline phosphatase (AKP) in the group C and group T at 14 days were significantly higher than those at 0 day, and significantly higher than those in the group K (P<0.05). In the liver (SOD) and the total antioxidant capacity (T-AOC) in the group C at 14 days were significantly higher than those at 0 day (P<0.05), and the content of malondialdehyde (MDA) in the group C was lower than that at 0 day and 7 days. In the challenge test, the mortality rate of juvenile koi carp in the group K within 24 hours was 41.7%. The mortality rate in the group C was 18.3% and the protection rate was 56%. The mortality rate in the group T was 21.7% and the protection rate was 48%. In the serum, the interleukin-13 (IL-13) content in the group C at 48 hours was significantly higher than that at 24 hours and 96 hours (P<0.05), and the immunoglobulin M (IgM) content in the group C and group T at 48 hours was significantly higher than that at 24 hours (P<0.05). In the liver, the content of interleukin-4 (IL-4), IL-13, and IgM in the group C at 48 hours were significantly higher than those at 24 hours, and the content of IL-13 in the group C was significantly higher than that of the group K (P<0.05). The experiment indicates that adding Bupleuri radix and its extract to the diet for 14 days can effectively improve the physiological and biochemical indexes and anti-Aeromonas vickeri ability of juvenile koi carp, but the action time and effect of the two are different, and the effect of Bupleuri radix is more prominent. [ABSTRACT FROM AUTHOR]