1. AIE fluorescent nanozyme-based dual-mode biosensor for analysis of the bioactive component hypoxanthine in meat products.
- Author
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Wu, Guojian, Luo, Jiaqi, Du, Chenxing, Zheng, Zhi, Zhang, Yang, Luo, Pengjie, Wu, Yongning, and Shen, Yizhong
- Subjects
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BIOACTIVE compounds , *MEAT , *HYPOXANTHINE , *BIOSENSORS , *XANTHINE oxidase - Abstract
The development of facile, low-cost reliable, and precise onsite assays for the bioactive component hypoxanthine (Hx) in meat products is significant for safeguarding food safety and public health. Herein, we proposed a smartphone-assissted aggregation-induced emission (AIE) fluorogen tetraphenylethene (TPE)-incorporated amorphous Fe-doped phosphotungstates (Fe-Phos@TPE) nanozyme-based ratiometric fluorescence-colorimetric dual-mode biosensor for achieving the onsite visual detection of Hx. When the Hx existed, xanthine oxidase (XOD) catalyzed Hx into H 2 O 2 to be further catalyzed into •OH by the prominent peroxidase activity of Fe-Phos@TPE at pH = 6.5, resulting in the oxidization of nonfluorescent o -phenylenediamine (OPD, naked-eye colorless) to be yellow fluorescent emissive 2,3-diaminophenazine (DAP, naked-eye dark yellow) at 550 nm as well as the intrinsic blue fluorescence of Fe-Phos@TPE at 440 nm to be decreased via inner-filter effect (IFE) action, thereby realizing a multi-enzyme cascade catalytic reaction at near-neutral pH to overcome the traditional acidity dependence-induced time-consuming and low sensitivity troublesome. • Synthesis of AIE fluorescent nanozyme Fe-Phos@TPE with high peroxidase-like activity at neutral pH. • Ratiometric fluorescence-colorimetric dual-mode analysis of bioactive component hypoxanthine. • The smartphone-based biosensor possessed a high sensitivity and fast kinetics. • The biosensor has been applied to the detection of hypoxanthine in meat products. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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