1. Development and optimization of an immunoassay for the detection of Hg(II) in lake water
- Author
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Xingrong Ju, Jie Zhang, Mingjie Wang, Changrui Xing, Rong He, Jin Xuexue, and Jian Yuan
- Subjects
food.ingredient ,Metal ions in aqueous solution ,lcsh:TX341-641 ,immunoassay ,Hg(II) ,tap water ,antibody ,lake water ,010501 environmental sciences ,01 natural sciences ,Polyvinyl alcohol ,Gelatin ,chemistry.chemical_compound ,food ,Tap water ,Pulmonary surfactant ,medicine ,Bovine serum albumin ,Original Research ,0105 earth and related environmental sciences ,Detection limit ,Chromatography ,biology ,medicine.diagnostic_test ,010401 analytical chemistry ,0104 chemical sciences ,chemistry ,Immunoassay ,biology.protein ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC50) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH3Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.
- Published
- 2019