1. Characterization of the promoter and 5'-UTR intron of oleic acid desaturase (FAD2) gene in Brassica napus
- Author
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Chang Ming Lu, Zhen Qian Zhang, Xian Meng Wu, Gang Xiao, Chang Fa Yin, She Yuan Chen, Chun Yun Guan, Rui Yang Liu, and Tai Long Tan
- Subjects
Fatty Acid Desaturases ,Five prime untranslated region ,Molecular Sequence Data ,Arabidopsis ,Gene Dosage ,Biology ,Genome ,chemistry.chemical_compound ,Transcription (biology) ,Gene Expression Regulation, Plant ,Genetics ,Promoter Regions, Genetic ,Gene ,Abscisic acid ,Phylogeny ,Regulation of gene expression ,Base Sequence ,Brassica napus ,Intron ,Temperature ,food and beverages ,Promoter ,General Medicine ,Plants, Genetically Modified ,Molecular biology ,chemistry ,5' Untranslated Regions ,Abscisic Acid - Abstract
In the present study, we characterized the transcriptional regulatory region (KF038144) controlling the expression of a constitutive FAD2 in Brassica napus . There are multiple FAD2 gene copies in B. napus genome. The FAD2 gene characterized and analyzed in the study is located on chromosome A5 and was designated as BnFAD 2A5-1. BnFAD 2A5-1 harbors an intron (1192 bp) within its 5′-untranslated region (5′-UTR). This intron demonstrated promoter activity. Deletion analysis of the BnFAD 2A5-1 promoter and intron through the β-glucuronidase (GUS) reporter system revealed that the − 220 to − 1 bp is the minimum promoter region, while − 220 to − 110 bp and + 34 to + 285 bp are two important regions conferring high-levels of transcription. BnFAD 2 transcripts were induced by light, low temperature, and abscisic acid (ABA). These observations demonstrated that not only the promoter but also the intron are involved in controlling the expression of the BnFAD 2A5-1 gene. The intron-mediated regulation is an essential aspect of the gene expression regulation.
- Published
- 2014