Search

A patent has been issued for a method of detecting protein-protein interactions in formalin-fixed paraffin-embedded tissue using caged haptens. This method involves forming enzyme-antibody conjugate complexes and detecting targets in the sample. The technology aims to enhance the ability to detect cellular markers for diagnostic and therapeutic purposes, particularly in cancer biology. The patent was filed by inventors from Tucson, Arizona, and assigned to Ventana Medical Systems Inc. [Extracted from the article]

A recent study conducted at the Medical University of Plovdiv in Bulgaria focused on using immunohistochemistry for Immunoglobulin G4 (IgG4) on paraffin sections as a diagnostic test for pemphigus, an autoimmune bullous disease. The study found that 98% of specimens from patients with pemphigus were immunoreactive for IgG4, indicating a high sensitivity of 98% and specificity of 90% for this diagnostic method. This method can be a valuable alternative when direct immunofluorescence examination is unavailable, as it does not require special equipment and provides permanent histology slides. [Extracted from the article]

A study conducted at Sun Yat-sen University in Guangzhou, China, examined the value of different scoring systems for predicting prognosis in paraffin-embedded donor kidney core biopsies. The research found that modified scoring systems, particularly focusing on glomerulosclerosis, interstitial fibrosis, tubular atrophy, and arteriolar hyalinosis, had better predictive capacities for estimated glomerular filtration rate (eGFR) compared to original scoring systems. The study suggests that a simple and pragmatic scoring system should be used to predict allograft prognosis in donor kidney biopsies with more than ten glomeruli, with further validation recommended in frozen sections. [Extracted from the article]

A patent application titled "Deparaffinization Of Tissue Utilizing Electric Field" was published online by inventors Lim and McCampbell. The application describes a method for preparing tissue embedded with paraffin by applying an electric field to remove the paraffin. This innovative approach aims to improve tissue processing in histology and related fields, potentially enhancing the efficiency and accuracy of tissue examination using analytical instruments like microscopes. [Extracted from the article]

A patent has been issued for a method and device for high-quality imaging of embedded tissue sections. The invention aims to improve the imaging of paraffin-embedded histopathology slides without removing the paraffin. The method involves using a layered structure to reduce optical back reflection and artifacts caused by reflecting light from the tissue section surfaces. The device includes a layered structure beneath the tissue section embedded in a solid material, which reflects light differently at different illumination angles to image the tissue section. The patent was assigned to the Trustees of Boston University. [Extracted from the article]

A patent application has been submitted for a new system and method for preparing and processing pathology slides. The invention aims to improve the accuracy and efficiency of histopathology procedures, which involve examining tissue samples for the diagnosis of diseases. The new system includes pathology slides with visibly perceptible separation marks, allowing for multiple tissue sample sections to be stained and analyzed on a single slide. This innovation has the potential to streamline the process and reduce errors in pathology diagnostics. The patent application was filed by inventors from Leavitt Medical Inc. and is currently awaiting approval. [Extracted from the article]

A patent application titled "Deparaffinization Of Tissue By Electric Field Generation And Ionization" has been published online. The application explains the process of preparing biological tissue for examination using a microscope, detailing the steps involved in fixing, dehydrating, infiltrating, embedding, and cutting the tissue. It emphasizes the importance of ensuring the tissue adheres to the microscope slide during staining. The application also describes a method and device for deparaffinizing tissue samples in a laboratory setting, involving the application of heat energy and an electric field to remove the paraffin. This invention aims to enhance the preparation of tissue samples for further analysis. [Extracted from the article]

Researchers at the University of Montpellier in France have conducted a study on monoclonal antibodies for protein detection in formalin-fixed and paraffin-embedded tissues. The study aimed to explore the use of naive antibody repertoires displayed on filamentous phages as a source of full-length antibodies for immunohistochemistry. The researchers isolated efficient antibody clones using in vitro panning techniques and identified a clone specific to HER2-positive breast cancer. However, the specificity was limited to the tissue used for selection and screening, highlighting the need for protocols that consider tissue diversity early in the selection process. The research has been peer-reviewed and published in the Journal of Immunological Methods. [Extracted from the article]

Agilent Technologies Inc. has been issued a patent for a method and apparatus for deparaffinization of tissue. The patent describes a process used in histology and histopathology to prepare tissue samples for examination under a microscope. The method involves generating an electric field that removes paraffin from the tissue by electrostatic force. The invention aims to improve the efficiency and accuracy of tissue preparation for analysis. [Extracted from the article]

Researchers at the University of Jinan in Jinan, People's Republic of China, have developed a pressure-responsive self-lubricating ice-phobic organogel with high mechanical strength. The organogels, made from fluorinated polynorbornene networks, exhibited excellent stability and durability, even under harsh conditions. They also demonstrated a reversible pressure-responsive effect, where the paraffin in the gels could migrate to form a self-lubricating layer on the surface under external force. The mechanical, anti-icing, and antifrosting properties of the organogels could be adjusted by varying the paraffin content. This study offers a convenient approach to developing smart anti-icing materials with high mechanical durability. [Extracted from the article]

A recent study conducted at the North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences in Meghalaya, India, explored the diagnostic value of immunofluorescence techniques on formalin-fixed, paraffin-embedded skin biopsy samples. The researchers compared the results of paraffin immunofluorescence with fresh frozen immunofluorescence and found that the two methods had a strong correlation. The study concluded that immunofluorescence on formalin-fixed paraffin-embedded tissue can be used as a salvage technique when immunofluorescence on the frozen section is insufficient or unavailable. However, it is important to note that the study had a small sample size and employed only one method of antigen retrieval. [Extracted from the article]

A patent has been issued to Leavitt Medical Inc. for a system and method related to pathological slides. The invention involves a substantially transparent substrate with multiple regions for receiving biopsy tissue sample sections. The substrate also includes visibly perceptible separation marks to distinguish between the regions. This technology aims to improve the preparation and processing of pathology slides, which are used in the study and diagnosis of diseases. [Extracted from the article]

Agilent Technologies Inc. has been issued a patent for a new method and device for preparing tissue samples for analysis. The traditional process involves multiple steps, but the new method aims to improve efficiency and accuracy by removing paraffin from the tissue without adding any liquid. This is done using heat energy and an electric field to melt and ionize the paraffin, and then removing it from the tissue. The patent, titled "Deparaffinization of tissue by electric field generation and ionization," was filed in February 2020 and published online in May 2024. Agilent Technologies Inc. is a healthcare technology company associated with this patent. [Extracted from the article]

A recent study conducted by researchers at the Evandro Chagas National Institute of Infectious Diseases in Brazil compared two diagnostic techniques, colorimetric in situ hybridization (CISH) and quantitative real-time polymerase chain reaction (qPCR), for detecting Leishmania infantum infection in dogs. The study found that both CISH and qPCR showed satisfactory sensitivities for diagnosing L. infantum in dogs, even in those without clinical signs. The combination of these two techniques increased the overall sensitivity for diagnosis. This research provides valuable insights into the diagnosis of zoonotic visceral leishmaniasis, which is caused by L. infantum and dogs serve as reservoirs for this parasite. [Extracted from the article]

This document discusses the development of a patent for a mold used to make alkali metal wax packets for atomic clocks. The advancement of micro-machining technologies has allowed for the miniaturization of atomic clocks, and the use of paraffin as a coating material enables further miniaturization and integration. The patent describes a method for making the wax packets using a silicon substrate and a wax layer, but acknowledges that this method lacks precise control. Subsequent technologies have improved the preparation of the wax packets by utilizing micromachining etching technology. The document highlights the need for a low-cost and practical mold for producing micro alkali metal wax packets and presents a mold assembly that addresses the inefficiency of current separation techniques. The mold assembly consists of packaging molds made of a silicon substrate, featuring wax packet receiving cavities and corrosion release holes. The document also provides a method for preparing the mold assembly and utilizing it to manufacture alkali metal wax packets. [Extracted from the article]

Agilent Technologies Inc. has been issued a patent for the preparation of tissue sections using fluorescence-based detection. The patent describes a method for determining the location of tissue in an embedded sample by irradiating the sample with light and detecting the fluorescence emission. The invention also includes an apparatus for slicing tissue sections from an embedded sample, which includes a microtome, light sources, and an optical system. This technology has applications in preserving tissue morphology, immunohistochemical staining, gene expression profiling, and disease studies. [Extracted from the article]

A patent application titled "Variable Power Cold Microwave Tissue Processor" has been published by the US Patent and Trademark Office. The application describes a method and apparatus for embedding cells in tissue samples more efficiently and quickly, while eliminating the use of hazardous chemicals like formalin. The proposed tissue processor utilizes a flow-through processing and embedding technique, along with a variable power microwave and a cooling system. The article also discusses a research report on the potential health risks associated with the use of paraffin as a fixative in pathology laboratories. The report suggests the need for further research in this area. [Extracted from the article]

A recent study conducted by researchers from the University of Toledo College of Medicine and Life Sciences focused on the extraction of high-quality DNA and RNA from archived formalin-fixed paraffin embedded (FFPE) tissue specimens. The study aimed to establish a protocol for this extraction process, using human FFPE biopsy tissues from various organs. The researchers modified existing kits and successfully obtained high-quality DNA and RNA, which were suitable for downstream genetic analysis. These findings suggest that silica-based membrane technology can be effective in yielding high-quality genetic material from FFPE tissues. [Extracted from the article]

Larger tissue samples are placed into cassettes having somewhat larger slotted openings which are nevertheless smaller than the tissue sample inside the cassette. "In accordance with another embodiment, an apparatus for holding a histologic tissue sample while sectioning the tissue sample in a microtome includes a tissue cassette having a recess including at least one side wall and a bottom wall. The tissue cassette is configured to hold the tissue sample, and is formed of a first material that can be successfully sectioned in a microtome, and resistant to degradation from solvents and chemicals used to fix and process the tissue sample during a histologic procedure. [Extracted from the article]

Chemicals, Endocrine Research, Health and Medicine, Hydrocarbons, Paraffin Keywords: Chemicals; Endocrine Research; Health and Medicine; Hydrocarbons; Paraffin EN Chemicals Endocrine Research Health and Medicine Hydrocarbons Paraffin 6208 6208 1 10/24/23 20231027 NES 231027 2023 OCT 27 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on endocrine research is now available. [Extracted from the article]

The printer as claimed in claim 11, wherein the printer comprises a transport apparatus (3) which transports the histology-laboratory consumable articles (16). The printer as claimed in claim 3, wherein the control apparatus (8) compares the image-pattern image signals received from the optical reading apparatus (13) with the image signals of the reference image pattern by image analysis and/or pattern recognition. The printer as claimed in claim 16, wherein the transport apparatus (3) transports the histology-laboratory consumable articles (16) printed on by the printing device (4) further, in particular to a removal device (7). The printer as claimed in claim 1, wherein the control apparatus (8) determines where a printing field is located on the histology-laboratory consumable article (16) on the basis of the image-pattern image signals. [Extracted from the article]

The incident light passes through the tissue section embedded in the solid material, and the layered structure with reduced reflection at each of the interfaces such one or more artifacts from reflecting the incident light from surfaces of the tissue section embedded in the solid material is reduced. Embedding tissue into paraffin blocks supports the tissue structure and enables very thin sections to be cut and mounted onto microscope slides for analysis. The layered structure is configured to reduce optical back reflections at an interface between the tissue section embedded in the solid material and the layered structure when an incident light is applied to the tissue section embedded in the solid material. The tissue section embedded in the solid material is disposed on a layered structure configured to reduce optical back reflection at an interface between the tissue section embedded in the solid material and the layered structure. [Extracted from the article]

Keywords: Health and Medicine; Hydrocarbons; Mineral Oil; Paraffin; Petrolatum EN Health and Medicine Hydrocarbons Mineral Oil Paraffin Petrolatum 6908 6908 1 08/21/23 20230825 NES 230825 2023 AUG 25 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on agriculture is now available. Health and Medicine, Hydrocarbons, Mineral Oil, Paraffin, Petrolatum. [Extracted from the article]

The system according to claim 1, wherein the RFID tag of each support is an NFC tag; the RFID tag of each tray is an NFC tag; said at least one RFID interrogator is an NFC interrogator; and said RFID antennas of said plurality of RFID antennas are NFC antennas. The system according to claim 1, wherein said RFID antenna for reading the RFID tag of the tray is distinct from said RFID antennas for reading the RFID tags of the supports. This is made possible, in particular, by said one or more demultiplexers, capable of sequentially activating a unique transmission line for each RFID antenna, in particular, so that only one antenna at a time is electrically connected to the RFID interrogator, while the other RFID antennas are electrically disconnected from the RFID interrogator. "Advantageously, the RFID reading occurs sequentially on different RFID antennas, allowing the system, in addition to perform normal identification, to know the position of an RFID tag, and therefore of a biological sample, in particular an histology sample, on the tray. [Extracted from the article]

The stack of histology cassettes of claim 1 wherein the length of tape holds the histology cassettes rigidly together by applying pressure to the histology cassettes. The stack of histology cassettes of claim 1 wherein the length of tape is configured so that the release region projects outwardly from the stack of histology cassettes. "In most modern devices, histology cassettes are marked with cassette data by a printer, which may be referred to as a histology cassette printer, but is herein referred to simply as a "printer". "Preferably, the length of tape holds the histology cassettes together, preferably rigidly together, by applying pressure to the histology cassettes (e.g. by tightly wrapping around the stack of histology cassettes), more preferably without the length of tape adhering to the histology cassettes. [Extracted from the article]

"Preferably, the mold assembly further includes a silicon pin mold, the silicon pin mold including a silicon substrate forming a main body of the silicon pin mold; "the silicon substrate including: "a substrate; and "silicon pins protruding outward from a surface of the substrate and configured to correspond to the wax packet receiving cavities; and "the silicon pins configured to form cavities for receiving the alkali metal on a side of the paraffin layer away from the release sacrificial layer. Direct physical peeling may lead to a decrease in the yield of the wax packets, and the separation by corroding the silicon substrate completely dissolves the wax packet mold, making the wax packet mold non-reusable. The mold assembly according to claim 1, wherein the corrosion release holes are through holes connecting the upper and lower side surfaces of the silicon substrate to directly reach the release sacrificial layer. [Extracted from the article]

"It would be desirable to combine the water bath, slide heater, tissue orienter and/or slide basket heater into one unit to improve the throughput of the staining process by pre-warming the slides while they are being prepared and organized in the slide basket." "A horizontal heating plate (also referred to as a slide heater), which can be used along with a water bath in tissue processing, may be a metal plate that is typically heated to about 40 C. for drying a tissue mounted slide. The tissue processing station of claim 1, further comprising a slide basket heater positioned within an opening defined on the second horizontally oriented surface, the slide basket heater containing a cavity for receiving a plurality of vertically oriented slides, and one or more heating elements for heating the cavity. [Extracted from the article]

The last step of processing is to infiltrate the tissue with melted paraffin (usually at about 60° C.) and then cool the tissue and paraffin to room temperature. Orientation of the tissue matters at this point, as the tissue closest to the bottom of the mold will be the tissue that is first cut by a microtome. The tissue portion(s) are placed loosely into a tissue cassette that is labeled with a barcode containing information associated with the tissue, and the tissue cassette is closed to secure the tissue therein. "A typical tissue cassette has outer dimensions of about 28.5 x 41 x 6.7 millimeters (mm), a hinged lid, and flow-through slots or holes to allow the tissue to be immersed in liquid while remaining securely contained inside the tissue cassette. [Extracted from the article]

The support device of claim 11, wherein moving the lid and the tissue cassette from the first position to the second position elastically deforms the lid-frame connector. Once the tissue sample or samples are removed and sent to a pathology laboratory, the tissue will go through a series of procedures performed by a histotechnician and, ultimately, a pathologist, in order to diagnose one or more conditions associated with the tissue. "In additional or alternative aspects, the tissue cassette may include a cassette closure element and the lid may include a lid closure element, the cassette closure element and the lid closure element being configured, when engaged, to secure the lid to the tissue cassette. [Extracted from the article]

In other situations involving very small tissue samples, the samples are placed into a bag that resembles a tea bag that prevents the smallest tissue samples from escaping. Once the fluids have been removed from the tissue samples, the processing machine immerses the tissues samples in a bath of a hardenable material such as molten paraffin (i.e., a form of wax) so that the interstices in the tissue are replaced with paraffin. "In additional or alternative aspects, the tissue cassette may include a cassette closure element and the lid may include a lid closure element, the cassette closure element and the lid closure element being configured, when engaged, to secure the lid to the tissue cassette. [Extracted from the article]

The tissue will undergo a process known as "grossing-in" in the pathology lab during which a histotechnician will retrieve the tissue sample from the container, typically cut the tissue into appropriate sizes for tissue processing, place individual samples into the appropriate sized small plastic tissue cassettes, and assign tracking numbers to each cassette. In other situations involving very small tissue samples, the samples are placed into a bag that resembles a tea bag that prevents the smallest tissue samples from escaping. "In additional or alternative aspects, the tissue cassette may include a cassette closure element and the lid may include a lid closure element, the cassette closure element and the lid closure element being configured, when engaged, to secure the lid to the tissue cassette. [Extracted from the article]

Rochester, State:New York, United States, North and Central America, Chemicals and Chemistry, Hydrocarbons, Paraffin Keywords: Rochester; State:New York; United States; North and Central America; Chemicals and Chemistry; Hydrocarbons; Paraffin EN Rochester State:New York United States North and Central America Chemicals and Chemistry Hydrocarbons Paraffin 2441 2441 1 03/23/23 20230303 NES 230303 2023 MAR 3 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Chemicals and Chemistry. [Extracted from the article]

The support device of claim 28, wherein the frame further comprises at least one stop, wherein when the lid and the tissue cassette are moved from the first position to the second position, the stop prevents the lid and the tissue cassette from moving beyond the second position. "In additional or alternative aspects, the tissue cassette may include a cassette closure element and the lid may include a lid closure element, the cassette closure element and the lid closure element being configured, when engaged, to secure the lid to the tissue cassette. Once the tissue sample or samples are removed and sent to a pathology laboratory, the tissue will go through a series of procedures performed by a histotechnician and, ultimately, a pathologist, in order to diagnose one or more conditions associated with the tissue. [Extracted from the article]

After Ag nanoparticles were modified, the t1/2 and F(T) and the activation energy of AEP were increased which were higher than that of 0-AEP but still lower than that of pure PW, revealing that the crystal growth and nucleation of PW were restricted by the surface interaction between Ag and PW." In this work, the non-isothermal crystallization behavior of Ag nanoparticles modified expanded graphite/paraffin (AEP) composited PCMs was studied to analyze the effect of expanded graphite (EG) and Ag nanoparticles on the crystallization process of paraffin wax (PW).". [Extracted from the article]

Biomarkers, Diagnostics and Screening, Health and Medicine, Hydrocarbons, Proteomics, Paraffin Keywords: Biomarkers; Diagnostics and Screening; Health and Medicine; Hydrocarbons; Paraffin; Proteomics EN Biomarkers Diagnostics and Screening Health and Medicine Hydrocarbons Paraffin Proteomics 2023 FEB 10 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in biomarkers. [Extracted from the article]