Search

A taxonomic identification using polyphasic approach was performed on strain TH16-21T, which was isolated from the interfacial sediment of Taihu Lake, PR China. Strain TH16-21T was Gram-stain-negative, aerobic, rod-shaped and catalase-positive. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that strain TH16-21T was classified within the genus of Flavobacterium . The 16S rRNA gene sequence of strain TH16-21T showed the highest similarity to Flavobacterium cheniae NJ-26T (98.9 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain TH16-21T and F. cheniae NJ-26T were 91.2 and 45.9 %, respectively. The respiratory quinone was menaquinone 6. The major cellular fatty acids (>10 %) comprised iso-C15 : 0, iso-C16 : 0, iso-C15 : 1 G and iso-C16 : 0 3-OH. The genomic DNA G+C content was 32.2 mol%. Phosphatidylethanolamine, six amino lipids and three phospholipids were the main polar lipids. Based on the phenotypic features and phylogenetic position, a novel species with the name Flavobacterium lacisediminis sp. nov. is proposed. The type strain is TH16-21T (=MCCC 1K04592T=KACC 22896T).

A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137

A novel actinobacterial strain, designated X17T, was isolated from the sediment of Taihu Lake in China and its taxonomic position was investigated by using a polyphasic approach. The isolate formed milky-white colonies comprising aerobic, Gram-stain-positive, rod-shaped cells. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism belonged to the genus Nocardioides and consistently formed a distinct cluster with Nocardioides agariphilus JCM 16020T and Nocardioides islandensis MSL 26T, sharing 95.5 and 94.8 % 16S rRNA gene sequence similarities, respectively. The genomic DNA G+C content was 69.9 mol%. Chemotaxonomically, the isolate contained ll-diaminopimelic acid in the cell-wall peptidoglycan, MK-8 (H4) as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids found in the cell wall. The major fatty acids were 18 : 1 ω9c (38.6 %), 16 : 0 iso (20.3 %), 15 : 0 iso (6.8 %) and 18 : 0 (5.8 %). Based on its physiological, biochemical and chemotaxonomic characteristics, the strain represents a novel species of the genus Nocardioides , for which the name Nocardioides taihuensis sp. nov. (type strain X17T=CGMCC 4.7318T=NBRC 112321T) is proposed.

A Gram-negative, short ovoid- to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain KS-11(T)) was isolated from sediment of the eutrophic Guanting reservoir in Beijing, China. Colonies grown on R2A agar plates were circular, convex and colourless to orange. The strain grew in the presence of up to 1 % NaCl (optimum, 0 % NaCl). Growth occurred at 25-40 degrees C (optimum, 28-37 degrees C) and at pH 6.0-9.5 (optimum, pH 7.5-9.0). On the basis of 16S rRNA gene sequence similarity, strain KS-11(T) was shown to belong to the class Alphaproteobacteria, being closely related to Paracoccus marinus (96.9 % 16S rRNA gene sequence similarity), followed by Paracoccus koreensis (96.8 %), Paracoccus solventivorans (96.8 %), Paracoccus alkenifer (96.2 %) and Paracoccus kocurii (95.8 %). The major fatty acids of strain KS-11(T) were summed feature 7 (C(18 : 1)omega7c/omega9t/omega12t) (83.8 %) and C(18 : 0) (6.5 %) and the G+C content of the genomic DNA was 69.0 mol%. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain KS-11(T) represents a novel species of the genus Paracoccus, named Paracoccus chinensis sp. nov. The type strain is KS-11(T) (=CGMCC 1.7655(T)=NBRC 104937(T)).

A Gram-negative, rod-shaped, yellow-pigmented bacterium, designated strain R2A-7(T), was isolated from sediment of the eutrophic Taihu Lake in Jiangsu Province, China. The isolate was subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequences placed strain R2A-7(T) within the genus Flavobacterium in the family Flavobacteriaceae. The highest sequence similarity was found with Flavobacterium saliperosum (98.3 %), followed by other Flavobacterium species with similarities96.0 %. The major fatty acids (5 %) were 15 : 0 iso, 17 : 1 iso omega9c, 17 : 0 iso 3-OH, 15 : 1 iso G and 15 : 0 iso 3-OH. The G+C content of the genomic DNA of strain R2A-7(T) was 37.7 mol%. The DNA-DNA relatedness value with F. saliperosum CGMCC 1.3801(T) was 40.6 %. Molecular and phenotypic data suggest that strain R2A-7(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium cauense is proposed. The type strain is R2A-7(T) (=CGMCC 1.7270(T)=NBRC 104929(T)).

A novel actinobacterial strain, 3-wff-81T, was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain 3-wff-81T belonged to the genus Geodermatophilus , with Geodermatophilus soli PB34T (99.1 % similarity) and Geodermatophilus terrae PB261T (98.3 % similarity) as closest relatives. The major fatty acids were 16 : 0 iso, 15 : 0 iso, 17 : 1ω8c and 14 : 0 iso. The predominant menaquinones were MK-9(H4) and MK-9. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 73.2 mol%. DNA–DNA relatedness values with G. soli PB34T and G. terrae PB261T were 42.8 % and 39.6 %, respectively. Based on the physiological, biochemical and chemotaxonomic data, it is proposed that strain 3-wff-81T represents a novel species named Geodermatophilus taihuensis sp. nov. with 3-wff-81T ( = CGMCC 1.12303T = NBRC 109416T) as the type strain.

An orange-pigmented, Gram-reaction-positive, non-spore-forming, halophilic, alkali-tolerant rod, designated strain halo-2T, was isolated from sediment of Xiarinaoer soda lake, in China’s Inner Mongolia Autonomous Region. Strain halo-2T grew in a complex medium with 3–30 % (w/v) NaCl and at pH 5–10. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids were anteiso-C15 : 0 (43.6 %), anteiso-C17 : 0 (14.8 %) and iso-C15 : 0 (6.8 %) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of the novel strain was 48.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-2T was most closely related to Bacillus agaradhaerens DSM 8721T (93.9 % sequence similarity). However, strain halo-2T could be clearly differentiated from its closest phylogenetic relatives on the basis of several phenotypic, genotypic and chemotaxonomic characteristics. Strain halo-2T therefore represents a novel species in a new genus for which the name Salisediminibacterium halotolerans gen. nov., sp. nov. is proposed. The type strain of the type species is halo-2T ( = CGMCC 1.7654T = NBRC 104935T).

A novel strain, NJ-8(T), was isolated from sediment of the eutrophic Taihu Lake (China) and was subjected to a taxonomic study by using a polyphasic approach. The novel strain was aerobic and formed orange-pigmented colonies on R2A agar. Cells were Gram-negative single rods that were motile by means of gliding. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NJ-8(T) belonged to the phylum Bacteroidetes, with Niastella yeongjuensis DSM 17621(T) (92.3 % gene sequence similarity) and Niastella koreensis DSM 17620(T) (92.0 % similarity) as its closest relatives. The major fatty acids (5 % of the total) were iso-C(15 : 0) (27.8 %), iso-C(15 : 1) G (15.4 %), iso-C(17 : 0) 3-OH (14.5 %) and summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 13.9 %). The genomic DNA G+C content was 46.6 mol%. Based on molecular and phenotypic data, strain NJ-8(T) is considered to represent a novel species of a new genus, for which the name Lacibacter cauensis gen. nov., sp. nov. is proposed. The type strain of the type species of the genus, Lacibacter cauensis, is NJ-8(T) (=CGMCC 1.7271(T)=NBRC 104930(T)).

A Gram-negative, rod-shaped, yellow pigmented bacterium, strain NJ-26(T), was isolated from sediment of the eutrophicated Guanting Reservoir in Beijing, China. A phylogenetic analysis based on 16S rRNA gene sequences placed strain NJ-26(T) within the genus Flavobacterium in the family Flavobacteriaceae. The highest sequence similarity was found with Flavobacterium cucumis R2A45-3(T) (97.7%). The major fatty acids (5%) of the isolate were iso-C(15:0), iso-C(17:1)omega9c, C(15:0), iso-C(17:0) 3-OH, iso-C(15:1) G and iso-C(15:0) 3-OH. The G+C content of the genomic DNA was 40.6 mol%. The DNA-DNA relatedness value with F. cucumis R2A45-3(T) was 5.4%. Molecular and phenotypic data suggest that strain NJ-26(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium cheniae sp. nov. is proposed. The type strain is NJ-26(T) (=CGMCC 1.6844(T) =NBRC 103934(T)).