1. Selenoprotein Expression in Macrophages Is Critical for Optimal Clearance of Parasitic Helminth Nippostrongylus brasiliensis
- Author
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Joseph F. Urban, Shakira M. Nelson, K. Sandeep Prabhu, Ashley E. Shay, Bradley A. Carlson, and Jamaal James
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Immunology ,Macrophage polarization ,Prostaglandin ,Biochemistry ,Mice ,Selenium ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,parasitic diseases ,medicine ,Animals ,Nippostrongylus ,Nippostrongylus brasiliensis ,Selenoproteins ,Molecular Biology ,Strongylida Infections ,chemistry.chemical_classification ,biology ,Prostaglandin D2 ,Macrophages ,Cell Biology ,Macrophage Activation ,biology.organism_classification ,Small intestine ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,chemistry ,Dietary Supplements ,biology.protein ,Cyclooxygenase ,Selenoprotein - Abstract
The plasticity of macrophages is evident in helminthic parasite infections, providing protection from inflammation. Previously we demonstrated that the micronutrient selenium induces a phenotypic switch in macrophage activation from a classically activated (pro-inflammatory; M1/CAM) toward an alternatively activated (anti-inflammatory; M2/AAM) phenotype, where cyclooxygenase (COX)-dependent cyclopentenone prostaglandin J2 (15d-PGJ2) plays a key role. Here, we hypothesize that dietary selenium modulates macrophage polarization toward an AAM phenotype to assist in the increasing clearance of adult Nippostrongylus brasiliensis, a gastrointestinal nematode parasite. Mice on a selenium-adequate (0.08 ppm) diet significantly augmented intestinal AAM presence while decreasing adult worms and fecal egg production when compared with infection of mice on selenium-deficient (
- Published
- 2016