1. Assay of the antiangiogenic compound TNP-470, and one of its metabolites, AGM-1883, by reversed-phase high-performance liquid chromatography in plasma.
- Author
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Figg WD, Yeh HJ, Thibault A, Pluda JM, Itoh F, Yarchoan R, and Cooper MR
- Subjects
- Chromatography, High Pressure Liquid statistics & numerical data, Cyclohexanes, Epoxy Compounds blood, Humans, Magnetic Resonance Spectroscopy, O-(Chloroacetylcarbamoyl)fumagillol, Quinolines, Sensitivity and Specificity, Sesquiterpenes chemistry, Acetamides blood, Antibiotics, Antineoplastic blood, Chromatography, High Pressure Liquid methods, Sesquiterpenes blood, Spiro Compounds blood
- Abstract
This paper describes a reversed-phase, high-performance liquid chromatographic (HPLC) method for the isolation, detection, and quantification of TNP-470 (I) and one of its active metabolites, AGM-1883 (II), from plasma. These compounds are initially extracted from plasma with an organic solvent and then separated from one another on a C18 column. Those fractions eluting from the C18 column and containing either I or II are then derivatized through their epoxide moieties with sodium 8-quinolinethiolate (SQT). This derivatization produces fluorescent species that are isolated and quantified by a second reversed-phase HPLC analysis. The assay yields a lower limit of reliable quantification of 2.5 ng/ml and is linear to a concentration at least as high as 160 ng/ml. The inter-assay percent coefficient of variation is less than 18%.
- Published
- 1994
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