1. Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples
- Author
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Rusung Tan, Peter Tilley, Ghada N. Al-Rawahi, Mohammad Rubayet Hasan, and Eva Thomas
- Subjects
Microbiology (medical) ,Time Factors ,Temperature ,RNA ,Bacteriology ,Biology ,Real-Time Polymerase Chain Reaction ,Molecular biology ,Specimen Handling ,chemistry.chemical_compound ,Cerebrospinal fluid ,Real-time polymerase chain reaction ,Molecular Diagnostic Techniques ,Biochemistry ,chemistry ,Nucleic Acids ,Short term stability ,Nucleic acid ,Humans ,Pathogen ,DNA ,RNA amplification - Abstract
The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and โ80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( P < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples.
- Published
- 2012