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Start Over Topic antigens Publication Year Range More than 50 years ago Journal journal of immunological methods
2 results

1. Rapid quantitation of the immunopreciptin reaction in agarose gel by electroimmunodiffusion with paper discs

Author

Rene J. Duquesnoy

Subjects
Paper, Immunodiffusion, Chromatography, biology, medicine.diagnostic_test, Immune Sera, Immunology, Serum albumin, Immunoelectrophoresis, Immune sera, chemistry.chemical_compound, chemistry, Antigen, biology.protein, medicine, Methods, Immunology and Allergy, Agarose, Animals, Rabbits, Antigens, Serum Albumin
Abstract

Paper disc-electroimmunodiffusion in agarose gel provides a rapid and sensitive quantitation of the immunoprecipitin reaction.

Published
1973

2. Immunoephelometric determination of fibrinogen and its derivatives in plasma

Author

P. Wolf and G.W. Farrell

Subjects
Immunodiffusion, Nitrogen, Immunology, Antigen-Antibody Complex, Fibrinogen, Fibrin, Antibodies, chemistry.chemical_compound, Iodine Isotopes, medicine, Methods, Immunology and Allergy, Animals, Humans, Streptokinase, Fibrinolysin, Antigens, Trisodium citrate, Reproducibility, Chromatography, Sheep, biology, Immune Sera, Immunochemistry, Gamma globulin, Plasma, chemistry, Solubility, biology.protein, Blood Coagulation Tests, gamma-Globulins, medicine.drug, Densitometry
Abstract

An immunonephelometric method for determination of fibrinogen and fibrin degradation products in plasma is described. Test plasms are diluted 1/600 in a solution containing 17 g NaCl and 3.8 g trisodium citrate/100 ml. To 3 cm 3 of this mixture 0.2 cm 3 of a concentrated antifibrin gamma globulin preparation is added. The reading of this sample minus the γ-globulin blank in a Thorp Nephelometer is directly proportional to the fibrinogen content of the plasma or to its FDP concentration in terms of intact fibrinogen. This paper examines the mechanism, accuracy and reproducibility of this method.

Published
1972