1. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
- Author
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Viladomiu, Monica, Bassaganya-Riera, Josep, Tubau-Juni, Nuria, Kronsteiner, Barbara, Leber, Andrew, Philipson, Casandra W., Zoccoli-Rodriguez, Victoria, and Hontecillas, Raquel
- Subjects
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MACROPHAGES , *HELICOBACTER pylori , *COLONIZATION , *LIPOSOMES , *T cells - Abstract
Helicobacter pylori, the dominant member of the human gastric microbiota, elicits immunoregulatory responses implicated in protective versus pathological outcomes. To evaluate the role of macrophages during infection, we employed a system with a shifted proinflammatory macrophage phenotype by deleting PPARγ in myeloid cells and found a 5- to 10-fold decrease in gastric bacterial loads. Higher levels of colonization in wild-type mice were associated with increased presence of mononuclear phagocytes and in particular with the accumulation of CD11b+F4/80hiCD64+CX3CR1+ macrophages in the gastric lamina propria. Depletion of phagocytic cells by clodronate liposomes in wild-type mice resulted in a reduction of gastric H. pylori colonization compared with nontreated mice. PPARγ-deficient and macrophage-depleted mice presented decreased IL-10-mediated myeloid and T cell regulatory responses soon after infection. IL-10 neutralization during H. pylori infection led to increased IL-17-mediated responses and increased neutrophil accumulation at the gastric mucosa. In conclusion, we report the induction of IL-10-driven regulatory responses mediated by CD11b+F4/80hiCD64+CX3CR1+ mononuclear phagocytes that contribute to maintaining high levels of H. pylori loads in the stomach by modulating effector T cell responses at the gastric mucosa. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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