1. Chemometric analysis of porcine, bovine and ovine heparins.
- Author
-
Ouyang, Yilan, Han, Xiaorui, Yu, Yanlei, Chen, Jianle, Fu, Li, Zhang, Fuming, Linhardt, Robert J., Fareed, Jawed, Hoppensteadt, Debra, Jeske, Walter, Kouta, Ahmed, Zhang, Zhenqing, and Xia, Ke
- Subjects
- *
CHEMOMETRICS , *HEPARIN , *DISACCHARIDES , *ANTICOAGULANTS , *POLYSACCHARIDES - Abstract
Highlights • 1H-NMR of heparins from different animals show distinct clusters in PCA and k means analysis. • Disaccharides components of heparins from different animals show distinct clusters in PCA and k means. • Tetrasaccharides components of heparins from different animals show distinct clusters in PCA and k means. • Chemometric analysis could help predict the heparin animal source and detect blending. Abstract Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF