1. Arabidopsis thaliana E3 ligase AIRP4 is involved in GA synthesis.
- Author
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Wang, Tao, Zhou, Qin, Wu, Xiaobo, Wang, Duo, Yang, Liang, Luo, Wenmin, Wang, Jianmei, Yang, Yi, and Liu, Zhibin
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UBIQUITIN ligases , *ARABIDOPSIS thaliana , *GIBBERELLINS , *SYNTHETIC genes , *GIBBERELLIC acid , *ABSCISIC acid , *GERMINATION , *ROOT growth - Abstract
Arabidopsis abscisic acid ABA-Insensitive RING Proteins (AtAIRP1–4) are RING E3s that play significant roles in ABA-signaling pathways. However, it is still unclear whether they have other functions. Here, AtAIRP4 was determined to play a role in response to gibberellin A3 (GA 3) in Arabidopsis thaliana. After proAtAIRP4::GUS transgenic lines were treated with GA 3 , the GUS activity decreased in hypocotyls. Increased hypocotyl elongation in response to GA 3 seen in WT was not observed in the AtAIRP4 -overexpression lines, whereas AtAIRP4 -overexpression lines were hypersensitive to Paclobutrazol (PAC, an inhibitor of GA biosynthesis) during the seed germination stage. Additionally, AtAIRP4 -overexpressing lines showed the lowest level of primary root elongation in the presence of GA 3. The levels of endogenous GA 3 in 35S::AtAIRP4 lines were lower than those in wild-type. In addition, among the plants, the mRNA levels of the GA synthetic gene GIBBERELLIN 20-OXIDASE1 (GA20ox1) was the lowest in overexpressing line. However, the expression of the response gene DELLA RGA-LIKE3 (RGL3) was the highest in overexpressing lines after treatment with GA 3. Thus, AtAIRP4 plays a negative role in GA-mediated hypocotyl elongation and root growth, and it inhibits the synthesis of endogenous biologically active GA 3 to some extent. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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