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Research has been conducted on the RNA oligomer mimicking hammerhead ribozyme metal ion-binding motif. Results indicate that this oligomer folds into a confirmation which possess metal ion binding property identical to that of the intact ribozyme.

Tandem G.A pairs and flanking C.G pair in hammerhead ribozymes were studied to determine the capture of structurally and catalytically significant metal ions.

The chemical structure of the T-[Hg.sup.II]-T pair is determined based on the [super 15]N NMR studies in which [super 15]N-[super 15]N J-coupling across [Hg.sup.II] ([super 2][J.sub.NN]) is seen for this T-[Hg.sup.II]-T pair. The results have shown a novel metal ion-binding mode for DNA molecules that includes the imino proton-metal exchange process.

A study reports an alternative method for generating metal-mediated base pairs in deoxyribonucleic acid (DNA) duplexes using only naturally occurring pyrimidine bases. Thymine-thymine (T--T) mispairs in DNA duplexes selectively capture Hg(super II) ions, and the metal-meidated thymine-Hg(super II)-thymine base pairs form in DNA duplexes.

Determines the metal ion binding site of an RNA oligomer. Use of nuclear magnetic resonance spectroscopy in determining the binding site; Perturbation of the metal ion-binding motif; Changes of the electron density distribution of the guanine.

The article discusses the mismatch in a DNA duplex between the natural base thymine-thymine (T-T) that bind to mercury (Hg). In this study, the authors determined the chemical structure of the T-Hg-T pair based from the nitrogen (N) Nuclear magnetic resonance (NMR) studies where N-N J-coupling over Hg was observed. It was reported that the chemical shift changes in N NMR are useful to the detection of hydrogen-bond formations and N-N J-coupling across hydrogen bond.

Determines whether or not a metal ion would bind with the phosphoryl oxygen at the cleavage site of a hammerhead ribozyme with the use of nuclear magnetic resonance (NMR) spectroscopy. Extent of the perturbations of NMR signals from phosphorothioates; Absence of any change in NMR signals at the cleavage site upon the addition of excess metal ions.

The article provides information on a study that describes the stabilization of DNA duplexes via the formation of a Hg2mediated base pair. The study basically investigated the interactions of metal ions with nucleic acids, nucleosides and nucleobases. The article also presents the figure of thermally induced transition profiles of DNA duplex. According to the study, the transition profiles shifted to higher temperatures in the presence of Hg2 ions. The study also informed about some of the metals that are known to bind to nucleic acids.