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1. Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus.

Author

Orlova OV, Glazkova DV, Tsyganova GM, Antoshkina IV, Mintaev RR, Tikhonov AS, Bogoslovskaya EV, and Shipulin GA

Subjects
Animals, Cell Line, Humans, Real-Time Polymerase Chain Reaction, Antigens, Viral, Genetic Vectors, SARS-CoV-2 genetics, Vaccinia virus isolation & purification
Abstract

Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Published
2021
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