1. Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues.
- Author
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He, Jie, Wu, Nan, Luo, Pengjie, Guo, Ping, Qu, Jianwen, Zhang, Shuying, Zou, Xin, Wu, Feiyao, Xie, Huijuan, Wang, Can, and Jiang, Wenxiao
- Subjects
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FOOD animals , *ENZYME-linked immunosorbent assay , *CLINDAMYCIN , *LINCOMYCIN , *MONOCLONAL antibodies - Abstract
In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC 50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra −/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC − MS/MS with a correlation coefficient > 0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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