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Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues.
- Source :
-
Meat Science . Mar2017, Vol. 125, p137-142. 6p. - Publication Year :
- 2017
-
Abstract
- In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC 50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra −/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC − MS/MS with a correlation coefficient > 0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03091740
- Volume :
- 125
- Database :
- Academic Search Index
- Journal :
- Meat Science
- Publication Type :
- Academic Journal
- Accession number :
- 120337656
- Full Text :
- https://doi.org/10.1016/j.meatsci.2016.11.024