1. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase
- Author
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Tobias Georgi, Volker F. Wendisch, and Doris Rittmann
- Subjects
glutamate production ,acetate metabolism ,Sucrose ,Lysine ,Carbohydrates ,amino-acids ,Malic enzyme ,Fructose 1,6-bisphosphatase ,Gene Expression ,Glutamic Acid ,Bioengineering ,in-vivo ,complex mixtures ,Applied Microbiology and Biotechnology ,fructose ,Corynebacterium glutamicum ,malic enzyme ,chemistry.chemical_compound ,Bacterial Proteins ,male ,Malate Dehydrogenase ,carbon-sources ,6-bisphosphatase ,biochemical-characterization ,glucose ,gene ,lysine production ,biology ,Glutamate receptor ,sucrose ,Fructose ,fbp ,Culture Media ,Fructose-Bisphosphatase ,flux ,pyruvate-carboxylase ,chemistry ,Biochemistry ,Fructolysis ,escherichia-coli ,biology.protein ,Carbohydrate Metabolism ,bacteria ,fructose-1 ,brevibacterium-flavum ,corynebacterium glutamicum ,Biotechnology - Abstract
In the biotechnological production Of L-lysine and L-glutamate by Corynebacterium glutamicum media based on glucose, fructose or sucrose are typically used. Glutamate production by C glutamicum ATCC13032 was very similar on glucose, fructose, glucose plus fructose and sucrose. In contrast, lysine production of genetically defined C glutamicum strains was significantly higher on glucose than on the other carbon sources. To test whether malic enzyme or fructose-1,6-bisphosphatase might limit growth and lysine on fructose, glucose plus fructose or sucrose, strains overexpressing either malE which encodes the NADPH-dependent malic enzyme or the fructose-1,6-bisphosphatase gene fbp were generated. Overexpression of malE did not improve lysine production on any of the tested carbon sources. Upon overexpression of fbp lysine yields on glucose and/or fructose were unchanged, but the lysine yield on sucrose increased twofold. Thus, fructose-1,6-bisphosphatase was identified as a limiting factor for lysine production by C glutamicum with sucrose as the carbon source. (c) 2005 Elsevier Inc. All rights reserved.
- Published
- 2005
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