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Your search keyword '"Reis-de-Oliveira G"' showing total 2 results
Start Over Author "Reis-de-Oliveira G" Journal methods in molecular biology clifton n j
2 results on '"Reis-de-Oliveira G"'

1. DIA-MS E to Study Microglial Function in Schizophrenia.

Author

Reis-de-Oliveira G, Carregari VC, and Martins-de-Souza D

Subjects
Cell Line, Chromatography, Liquid, Humans, Research Design, Microglia metabolism, Proteins analysis, Proteome, Proteomics, Schizophrenia metabolism, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
Abstract

Here, we describe a proteomic pipeline to use a human microglial cell line as a biological model to study schizophrenia. In order to maximize the proteome coverage, we apply two-dimensional liquid chromatography coupled with ultra-definition MS E mass spectrometry (LC-UDMS E ) using a data-independent acquisition (DIA) approach, with an optimization of drift time collision energy.

Published
2021
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2. A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue.

Author

Reis-de-Oliveira G, Fioramonte M, and Martins-de-Souza D

Subjects
Brain Diseases pathology, Cell Nucleus genetics, Cell Nucleus pathology, Humans, Proteome genetics, Subcellular Fractions pathology, Brain Diseases genetics, Chromatography, Liquid methods, Mass Spectrometry methods, Proteomics methods
Abstract

Here we describe a mass spectrometry-based proteomics workflow to discovery proteins differentially regulated in brains collected postmortem from mental, neurological, or substance abuse disorders (MNS) patients. One way to maximize protein detection is to carry out enrichment of cellular compartments such as the nucleus, mitochondria and cytosol. Subcellular fractionation improves proteome coverage and may shed light on the role of these organelles in the pathophysiology of MNS.

Published
2019
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