1. RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway
- Author
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Xuedong Sun, Chunxiao Dong, and Guanhua Chen
- Subjects
0301 basic medicine ,Lipopolysaccharides ,Cancer Research ,RHOA ,Cell ,Acute Lung Injury ,Apoptosis ,Lung injury ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,Viability assay ,RNA, Small Interfering ,Molecular Biology ,Wnt Signaling Pathway ,biology ,Wnt signaling pathway ,Cell cycle ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Catenin ,Gene Knockdown Techniques ,biology.protein ,Cancer research ,Molecular Medicine ,Reactive Oxygen Species ,rhoA GTP-Binding Protein - Abstract
Ras homolog family member A (RhoA) has been reported to be involved in numerous biological processes; however, the effects of RhoA on acute lung injury (ALI) have yet to be reported. The present study aimed to explore how RhoA affects cell viability, reactive oxygen species (ROS) activity and cell apoptosis in a cell model of lipopolysaccharide (LPS)‑induced ALI. An MTT assay, flow cytometry, reverse transcription‑quantitative polymerase chain reaction and western blotting were used to determine the effects of RhoA on cell viability, apoptosis and ROS activity. The results demonstrated that RhoA inactivation was able to promote cell viability, and decrease apoptosis and ROS activity of LPS‑treated cells. The results of western blotting indicated that RhoA activated the downstream Wnt/β‑catenin signaling pathway and inhibited the expression of apoptotic factors. These findings suggested that RhoA may be involved in ALI progression and could be a novel therapeutic target for this disease.
- Published
- 2016