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4 results on '"CANBIN LIN"'

1. Oncogenic miR-23a-5p is associated with cellular function in RCC

Author

Tao He, Hui Zeng, Peijie Chen, Yongqing Lai, Xiang Pan, Jing Quan, Shangqi Yang, Canbin Lin, Yulin Lai, and Lu Jin

Subjects
Adult, Male, 0301 basic medicine, Cancer Research, Cell Survival, Cell, Down-Regulation, Biology, urologic and male genital diseases, Biochemistry, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Cell Movement, Cell Line, Tumor, microRNA, Genetics, medicine, Humans, Carcinoma, Renal Cell, Molecular Biology, Aged, Cell Proliferation, medicine.diagnostic_test, Oncogene, Cell growth, Antagomirs, Middle Aged, Cell cycle, Molecular medicine, Kidney Neoplasms, Up-Regulation, MicroRNAs, HEK293 Cells, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, Molecular Medicine, Female
Abstract

In recent years, accumulating evidence has demonstrated that microRNAs (miRs, miRNAs) may serve an important role in the occurrence and development of tumors. miR‑23a‑5p has been confirmed as an oncogene in numerous diseases through gene chip analysis. However, as the most common type of renal tumor, the expression and function of miR‑23a‑5p in renal cell carcinoma (RCC) remains unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, and Cell Counting Kit‑8 (CCK‑8), wound scratch, Transwell, MTT and flow cytometry assays were performed to investigate the role of miR‑23a‑5p in RCC. The expression of miR‑23a‑5p in RCC tissue samples was significantly higher compared with that in normal tissue samples (P

Published
2017

2. Oncogenic miR-100-5p is associated with cellular viability, migration and apoptosis in renal cell carcinoma

Author

Tao He, Yong Wang, Jing Quan, Yongqing Lai, Liangchao Ni, Tao Wang, Shangqi Yang, Xueling Wu, Xiang Pan, Peijie Chen, Canbin Lin, Yulin Lai, and Liang Zhou

Subjects
0301 basic medicine, Adult, Male, Cancer Research, Cell Survival, Cell, Apoptosis, Biology, Biochemistry, Flow cytometry, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, Genetics, medicine, Humans, Viability assay, Molecular Biology, Carcinoma, Renal Cell, Cells, Cultured, Cell Proliferation, Neoplasm Staging, Oncogene, medicine.diagnostic_test, Cell growth, Oncogenes, Cell cycle, Middle Aged, Kidney Neoplasms, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Molecular Medicine, Female, Neoplasm Grading, A431 cells
Abstract

As influencing factors of genesis and progression in several types of human tumor, microRNAs (miRs) serves roles in the regulation of tumor cell viability, migration, and apoptosis. The present research aimed to investigate the association between the function of miR‑100‑5p and renal cell carcinoma (RCC). miR‑100‑5p expression was determined in RCC tissue and paired normal tissue samples using reverse transcription‑quantitative polymerase chain reaction. To assess the effects of miR‑100‑5p on cell viability, migration and apoptosis, multiple methods were used, including scratch wound assays, MTT assays, and flow cytometry. It was demonstrated that miR‑100‑5p was significantly upregulated in RCC tissue compared with in normal adjacent tissue samples. Furthermore, the viability and migration of 786‑O and, ACHN cells tranfected with miR‑100‑5p was significantly increased compared with the negative control group. In addition, miR‑100‑5p‑transfected 786‑O and ACHN cells demonstrated significantly reduced cellular apoptotic rates compared with the negative control group. To the best of our knowledge, the present study is the first to report an association between miR‑100‑5p and RCC. The results of the current study suggest that tumor oncogene miR‑100‑5p could be used as a diagnostic biomarker for RCC.

Published
2017

3. Oncogenic miR‑100‑5p is associated with cellular viability, migration and apoptosis in renal cell carcinoma.

Author

PEIJIE CHEN, CANBIN LIN, JING QUAN, YULIN LAI, TAO HE, LIANG ZHOU, XIANG PAN, XUELING WU, LIANGCHAO NI, SHANGQI YANG, TAO WANG, YONGQING LAI, and YONG WANG

Subjects
*RENAL cell carcinoma, *TUMORS, *CELL proliferation, *APOPTOSIS, *CANCER invasiveness
Abstract

As influencing factors of genesis and progression in several types of human tumor, microRNAs (miRs) serves roles in the regulation of tumor cell viability, migration, and apoptosis. The present research aimed to investigate the association between the function of miR‑100‑5p and renal cell carcinoma (RCC). miR‑100‑5p expression was determined in RCC tissue and paired normal tissue samples using reverse transcription‑quantitative polymerase chain reaction. To assess the effects of miR‑100‑5p on cell viability, migration and apoptosis, multiple methods were used, including scratch wound assays, MTT assays, and flow cytometry. It was demonstrated that miR‑100‑5p was significantly upregulated in RCC tissue compared with in normal adjacent tissue samples. Furthermore, the viability and migration of 786‑O and, ACHN cells tranfected with miR‑100‑5p was significantly increased compared with the negative control group. In addition, miR‑100‑5p‑transfected 786‑O and ACHN cells demonstrated significantly reduced cellular apoptotic rates compared with the negative control group. To the best of our knowledge, the present study is the first to report an association between miR‑100‑5p and RCC. The results of the current study suggest that tumor oncogene miR‑100‑5p could be used as a diagnostic biomarker for RCC. [ABSTRACT FROM AUTHOR]

Published
2017
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4. Oncogenic miR-23a-5p is associated with cellular function in RCC.

Author

JING QUAN, LU JIN, XIANG PAN, TAO HE, YULIN LAI, PEIJIE CHEN, CANBIN LIN, SHANGQI YANG, HUI ZENG, and YONGQING LAI

Subjects
ONCOGENIC viruses, MICRORNA, TUMORS, ONCOGENES, GENETIC transcription
Abstract

In recent years, accumulating evidence has demonstrated that microRNAs (miRs, miRNAs) may serve an important role in the occurrence and development of tumors. miR-23a-5p has been confirmed as an oncogene in numerous diseases through gene chip analysis. However, as the most common type of renal tumor, the expression and function of miR-23a-5p in renal cell carcinoma (RCC) remains unclear. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, and Cell Counting Kit-8 (CCK-8), wound scratch, Transwell, MTT and flow cytometry assays were performed to investigate the role of miR-23a-5p in RCC. The expression of miR-23a-5p in RCC tissue samples was significantly higher compared with that in normal tissue samples (P<0.01). Furthermore, the expression of miR-23a-5p in RCC cell lines (786O, ACHN and Caki-1) was significantly higher compared with that in the human embryo kidney 293T cell line, as determined using RT-qPCR (P<0.001). In addition, the results revealed that the upregulation of miR-23a-5p promoted the proliferation, migration and invasion of RCC cells, and inhibited RCC cell apoptosis. The downregulation of miR-23a-5p resulted in the reversal of the results described above. Additionally, it was observed that the downregulation of miR-23a-5p significantly promoted ACHN and 786O cell viability (P<0.001). The results of the present study suggest that miR-23a-5p is an oncogene in the occurrence and development of RCC and may be a novel therapeutic target for RCC. [ABSTRACT FROM AUTHOR]

Published
2017
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