1. H19 Overexpression Induces Resistance to 1,25(OH)2D3 by Targeting VDR Through miR-675-5p in Colon Cancer Cells
- Author
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Shuai Zuo, Tengyu Li, Pengyuan Wang, Shanwen Chen, Xin Wang, Yuanyuan Ma, Guowei Chen, Dingfang Bu, Yisheng Pan, Lie Sun, Jing Zhu, and Yucun Liu
- Subjects
0301 basic medicine ,Original article ,Cancer Research ,medicine.medical_specialty ,Colorectal cancer ,Drug Resistance ,Gene Expression ,Cell Cycle Proteins ,Biology ,medicine.disease_cause ,lcsh:RC254-282 ,Calcitriol receptor ,Proto-Oncogene Proteins c-myc ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Calcitriol ,Cell Movement ,RNA interference ,Cell Line, Tumor ,Internal medicine ,microRNA ,medicine ,Animals ,Humans ,Gene Regulatory Networks ,Receptor ,Cell Proliferation ,Regulation of gene expression ,Nuclear Proteins ,Cancer ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Xenograft Model Antitumor Assays ,female genital diseases and pregnancy complications ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,Endocrinology ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,embryonic structures ,Cancer research ,Receptors, Calcitriol ,RNA Interference ,RNA, Long Noncoding ,Carcinogenesis ,Signal Transduction - Abstract
The long noncoding (lnc) RNA H19 was involved in the tumorigenesis of many types of cancer. However, the role of H19 in the tumorigenesis of colon cancer has not been fully illustrated. Recent studies suggested a potential relationship between H19 and vitamin D receptor (VDR) signaling. Considering the pivotal role of VDR signaling in the colon epithelium both physiologically and pathologically, the correlation between H19 and VDR signaling may have an important role in the development of colon cancer. In this study, the correlation between H19 and vitamin D receptor (VDR) signaling and the underlying mechanisms in colon cancer were investigated both in vitro and in vivo. The results suggested that VDR signaling was able to inhibit the expression of H19 through regulating C-Myc/Mad-1 network. H19, on the other hand, was able to inhibit the expression of VDR through micro RNA 675-5p (miR-675-5p). Furthermore, H19 overexpression induced resistance to the treatment with 1,25(OH)2D3 both in vitro and in vivo. Together, these results suggested that H19 overexpression might be one of the mechanisms underlying the development of resistance to the treatment with 1,25(OH)2D3 in the advanced stage of colon cancer.
- Published
- 2017