1. Capsaicin-mediated apoptosis of human bladder cancer cells activates dendritic cells via CD91.
- Author
-
Montani, Maria Saveria Gilardini, D'Eliseo, Donatella, Cirone, Mara, Di Renzo, Livia, Faggioni, Alberto, Santoni, Angela, and Velotti, Francesca
- Subjects
- *
THERAPEUTIC use of capsaicin , *THERAPEUTIC use of proteins , *APOPTOSIS , *CELL physiology , *CELL receptors , *DENDRITIC cells , *NUTRITION , *SERIAL publications , *WESTERN immunoblotting , *PREVENTION , *TUMOR treatment ,BLADDER tumors - Abstract
Objectives: Immunostimulation by anticancer cytotoxic drugs is needed for long-term therapeutic success. Activation of dendritic cells (DCs) is crucial to obtain effective and long-lasting anticancer T-cell mediated immunity. The aim of this study was to explore the effect of capsaicin-mediated cell death of bladder cancer cells on the activation of human monocyte-derived CD1a+ immature DCs. Methods: Immature DCs (generated from human peripheral blood-derived CD14+ monocytes cultured with granulocyte-macrophage colony stimulating factor and interleukin-4) were cocultured with capsaicin (CPS)-induced apoptotic bladder cancer cells. DC activation was investigated using immunofluorescence and flow cytometric analysis for key surface molecules. In some experiments, CD91 was silenced in immature DCs. Results: We found that capsaicin-mediated cancer cell apoptosis upregulates CD86 and CD83 expression on DCs, indicating the induction of DC activation. Moreover, silencing of CD91 (a common receptor for damage-associated molecular patterns, such as calreticulin and heat-shock protein-90/70) in immature DCs led to the inhibition of DC activation. Conclusions: Our data show that CPS-mediated cancer cell apoptosis activates DCs via CD91, suggesting CPS as an attractive candidate for cancer therapy [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF