17 results on '"Kennelly, Edward J."'
Search Results
2. Anti-inflammatory Constituents from Caulis Trachelospermi#.
- Author
-
Song, Hongzhi, Tan, Jinni, Ma, Ruijing, Kennelly, Edward J, and Tan, Qingang
- Subjects
LIPOPOLYSACCHARIDES ,INTERLEUKINS ,ANTI-inflammatory agents ,ANIMAL experimentation ,WESTERN immunoblotting ,MASS spectrometry ,TUMOR necrosis factors ,PLANT extracts ,NITRIC oxide ,MOLECULAR structure ,ANALYTICAL chemistry ,PHARMACODYNAMICS - Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1 – 3) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22 , and 23 inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23 inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach.
- Author
-
Dake Zhao, Yana Shi, Xinyan Zhu, Li Liu, Pengzhang Ji, Chunlin Long, Yong Shen, and Kennelly, Edward J.
- Subjects
ACONITE ,ANALGESICS ,BIOMARKERS ,DRUG toxicity ,FACTOR analysis ,HERBAL medicine ,CHINESE medicine ,MOLECULAR structure ,QUALITY control ,RESEARCH funding ,IN vitro studies ,METABOLOMICS - Abstract
Despite their well-known toxicity, Aconitum species are important traditional medicines worldwide. Aconitum carmichaelii, known in Chinese as 附子 (fuzi), is an officially recognized traditional Chinese medicine with characteristic analgesic and anti-inflammatory activities, whose principal pharmacological ingredients are considered as aconitine-type diterpene alkaloids. Notwithstanding the long-recorded use of A. carmichaelii in traditional Chinese medicine, no single-entity aconitum alkaloid drug has been developed for clinical use. UPLC-Q-TOF-MS was used to investigate the marker compounds that can be used to differentiate A. carmichaelii from seven other Aconitum species collected in Yunnan Province. Nontargeted principle component analysis scores plots found that all the tested Aconitum species clustered into three distinct groups, and A. carmichaelii was significantly different chemically than the other seven species. Furthermore, the primary and lateral roots of A. carmichaelii also showed significant differences. Using orthogonal partial least squares discriminate analysis analysis, eight marker compounds were identified, including 14-acetylkarakoline, aconitine, carmichaeline, fuziline, hypaconitine, mesaconitine, neoline, and talatisamine. Four of these aconitum alkaloids, fuziline, hypaconitine, mesaconitine, and neoline, showed significant analgesic activity in a dose-dependent manner compared to the negative and positive controls. However, hypaconitine, mesaconitine, and neoline exhibited significant acute toxicity activity, while fuziline showed no acute toxicity in mice, suggesting the relative safety of this alkaloid. This study provides a good example of how to differentiate an authentic medicinal plant from common adulterants using a metabolomics approach, and to identify compounds that may be developed into new drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
4. Antioxidant and Cytotoxic Isoprenylated Coumarins fromMammea americana
- Author
-
Yang, Hui, primary, Protiva, Petr, additional, Gil, Roberto R., additional, Jiang, Bei, additional, Baggett, Scott, additional, Basile, Margaret J., additional, Reynertson, Kurt A., additional, Weinstein, I. Bernard, additional, and Kennelly, Edward J., additional
- Published
- 2005
- Full Text
- View/download PDF
5. Transport in Caco-2 Cell Monolayers of Antidiabetic Cucurbitane Triterpenoids from Momordica charantia Fruits.
- Author
-
Shi-Biao Wu, Yue, Grace G. L., Ming-Ho To, Keller, Amy C., Lau, Clara B. S., and Kennelly, Edward J.
- Subjects
DIET therapy for diabetes ,CELL culture ,FRUIT ,RESEARCH methodology ,MEDICINAL plants - Abstract
Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditionalmedicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cellmonolayers, andwere identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the baso-lateral sidewith apparent permeability coefficient (P
app ) values for 3-β-7-β,25-trihydroxycucurbita 5,23(E)-dien-19-al and momordicines I and II at 9.02 x 10-6 ,8.12 x 10-6 , and 1.68 x 10-6 cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
6. In Vitro Antiplasmodial Activity of Benzophenones and Xanthones from Edible Fruits of Garcinia Species.
- Author
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Lyles, James T., Negrin, Adam, Khan, Shabana I., Kan He, and Kennelly, Edward J.
- Subjects
BIOLOGICAL assay ,FRUIT ,HIGH performance liquid chromatography ,MASS spectrometry ,MEDICINAL plants ,MOLECULAR structure ,PARASITES ,TOXICITY testing ,PHYTOCHEMICALS ,IN vitro studies - Abstract
Species of Garcinia have been used to combat malaria in traditional African and Asian medicines, including Ayurveda. In the current study, we have identified antiplasmodial benzophenone and xanthone compounds from edible Garcinia species by testing for in vitro inhibitory activity against Plasmodium falciparum. Whole fruits of Garcinia xanthochymus, G. mangostana, G. spicata, and G. livingstonei were extracted and tested for antiplasmodial activity. Garcinia xanthochymus was subjected to bioactivity-guided fractionation to identify active partitions. Purified benzophenones (1-9) and xanthones (10-18) were then screened in the plasmodial lactate dehydrogenase assay and tested for cytotoxicity against mammalian (Vero) cells. The benzophenones guttiferone E (4), isoxanthochymol (5), and guttiferone H (6), isolated from G. xanthochymus, and the xanthones α-mangostin (15), β-mangostin (16), and 3-isomangostin (17), known from G. mangostana, showed antiplasmodial activity with IC
50 values in the range of 4.71-11.40 µM. Artemisinin and chloroquine were used as positive controls and exhibited IC50 values in the range of 0.01-0.24 µM. The identification of antiplasmodial benzophenone and xanthone compounds from G. xanthochymus and G. mangostana provides evidence for the antiplasmodial activity of Garcinia species and warrants further investigation of these fruits as dietary sources of chemopreventive compounds. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
7. New Monoterpene Lactones from Actaea cimicifuga.
- Author
-
Li-Juan Ma, Yue-Hu Wang, Gui-Hua Tang, Ye-Ling Wang, Chunhui Ma, Dastmalchi, Keyvan, Kennelly, Edward J., and Chun-Lin Long
- Subjects
MASS spectrometry methodology ,ORGANIC compound analysis ,INFRARED spectroscopy ,LIPASES ,MOLECULAR structure ,NUCLEAR magnetic resonance spectroscopy ,ORGANIC compounds ,PLANT stems ,TERPENES ,THIN layer chromatography ,PLANT extracts - Abstract
Three new monoterpene lactones, cimicifugolides A-C (1-3), along with a known one (4), were identified from the dried rhizome of Actaea cimicifuga L. that was used as traditional Chinese medicine for thousands of years with the Chinese common name of shengma. The structures of the new isolates were established using spectroscopic methods, including NMR, mass, UV, and IR spectra. The inhibition activity of compounds 1, 2, and 4 against pancreatic lipase was evaluated. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
8. Cucurbitane-Type Triterpenoids from Momordica charantia.
- Author
-
Ma, Jun, Whittaker, Paul, Keller, Amy C., Mazzola, Eugene P., Pawar, Rahul S., White, Kevin D., Callahan, John H., Kennelly, Edward J., Krynitsky, Alexander J., and Rader, Jeanne I.
- Abstract
One new cucurbitane-type triterpenoid glycoside, momordicoside U (
1 ), together with five known cucurbitane-type triterpenoids and related glycosides, 3β ,7β ,25-trihydroxycucurbita-5,23(E )-dien-19-al (2 ), momordicine I (3 ), momordicine II (4 ), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O -β -glucopyranoside (5 ), and kuguaglycoside G (6 ), were isolated from the whole plant ofMomordica charantia . Their structures were determined by chemical and spectroscopic methods. Momordicoside U (1 ) was evaluated for insulin secretion activity in anin vitro insulin secretion assay and displayed moderate activity. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
9. Actein and a Fraction of Black Cohosh Potentiate Antiproliferative Effects of Chemotherapy Agents on Human Breast Cancer Cells.
- Author
-
Einbond, Linda Saxe, Shimizu, Masahito, Nuntanakorn, Paiboon, Seter, Colette, Cheng, Rong, Jiang, Bei, Kronenberg, Fredi, Kennelly, Edward J., and Weinstein, I. Bernard
- Published
- 2006
- Full Text
- View/download PDF
10. Antioxidant and Cytotoxic Isoprenylated Coumarins from Mammea americana
- Author
-
Yang, Hui, Protiva, Petr, Gil, Roberto R., Jiang, Bei, Baggett, Scott, Basile, Margaret J., Reynertson, Kurt A., Weinstein, I. Bernard, and Kennelly, Edward J.
- Published
- 2005
- Full Text
- View/download PDF
11. Steroidal Saponins from Asparagus officinalis and Their Cytotoxic Activity.
- Author
-
Shao, Yu, Poobrasert, Onoomar, Kennelly, Edward J., Chin, Chee-Kok, Ho, Chi-Tang, Huang, Mou-Tuan, Garrison, Stephen A., and Cordell, Geoffrey A.
- Published
- 1997
- Full Text
- View/download PDF
12. Anti-inflammatory Constituents from Caulis Trachelospermi#.
- Author
-
Song, Hongzhi, Tan, Jinni, Ma, Ruijing, Kennelly, Edward J, and Tan, Qingang
- Subjects
- *
LIPOPOLYSACCHARIDES , *INTERLEUKINS , *ANTI-inflammatory agents , *ANIMAL experimentation , *WESTERN immunoblotting , *MASS spectrometry , *TUMOR necrosis factors , *PLANT extracts , *NITRIC oxide , *MOLECULAR structure , *ANALYTICAL chemistry , *PHARMACODYNAMICS - Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1 – 3) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22 , and 23 inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23 inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
13. Anti-inflammatory Constituents from Caulis Trachelospermi.
- Author
-
Song H, Tan J, Ma R, Kennelly EJ, and Tan Q
- Subjects
- Animals, Inflammation drug therapy, Lipopolysaccharides, Macrophages metabolism, Mice, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, RAW 264.7 Cells, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Apocynaceae chemistry, Plant Extracts chemistry, Plant Extracts pharmacology
- Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1: -3: ) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22: , and 23: inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23: inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides ., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
14. Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach.
- Author
-
Zhao D, Shi Y, Zhu X, Liu L, Ji P, Long C, Shen Y, and Kennelly EJ
- Subjects
- Aconitum toxicity, Analgesics pharmacology, Animals, Biomarkers analysis, Chromatography, High Pressure Liquid methods, Dose-Response Relationship, Drug, Female, Male, Metabolomics methods, Mice, Tandem Mass Spectrometry methods, Aconitum chemistry, Medicine, Chinese Traditional methods
- Abstract
Despite their well-known toxicity, Aconitum species are important traditional medicines worldwide. Aconitum carmichaelii , known in Chinese as (fuzi), is an officially recognized traditional Chinese medicine with characteristic analgesic and anti-inflammatory activities, whose principal pharmacological ingredients are considered as aconitine-type diterpene alkaloids. Notwithstanding the long-recorded use of A. carmichaelii in traditional Chinese medicine, no single-entity aconitum alkaloid drug has been developed for clinical use. UPLC-Q-TOF-MS was used to investigate the marker compounds that can be used to differentiate A. carmichaelii from seven other Aconitum species collected in Yunnan Province. Nontargeted principle component analysis scores plots found that all the tested Aconitum species clustered into three distinct groups, and A. carmichaelii was significantly different chemically than the other seven species. Furthermore, the primary and lateral roots of A. carmichaelii also showed significant differences. Using orthogonal partial least squares discriminate analysis analysis, eight marker compounds were identified, including 14-acetylkarakoline, aconitine, carmichaeline, fuziline, hypaconitine, mesaconitine, neoline, and talatisamine. Four of these aconitum alkaloids, fuziline, hypaconitine, mesaconitine, and neoline, showed significant analgesic activity in a dose-dependent manner compared to the negative and positive controls. However, hypaconitine, mesaconitine, and neoline exhibited significant acute toxicity activity, while fuziline showed no acute toxicity in mice, suggesting the relative safety of this alkaloid. This study provides a good example of how to differentiate an authentic medicinal plant from common adulterants using a metabolomics approach, and to identify compounds that may be developed into new drugs., Competing Interests: The authors declare no conflict of interest., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2018
- Full Text
- View/download PDF
15. Transport in Caco-2 cell monolayers of antidiabetic cucurbitane triterpenoids from Momordica charantia fruits.
- Author
-
Wu SB, Yue GG, To MH, Keller AC, Lau CB, and Kennelly EJ
- Subjects
- Biological Transport, Caco-2 Cells, Chromatography, High Pressure Liquid, Fruit chemistry, Glycosides chemistry, Glycosides isolation & purification, Humans, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Mass Spectrometry, Plants, Medicinal, Sterols chemistry, Sterols isolation & purification, Sterols metabolism, Triterpenes chemistry, Triterpenes isolation & purification, Diabetes Mellitus drug therapy, Glycosides metabolism, Hypoglycemic Agents metabolism, Momordica charantia chemistry, Triterpenes metabolism
- Abstract
Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditional medicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cell monolayers, and were identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the basolateral side with apparent permeability coefficient (P app) values for 3-β-7-β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicines I and II at 9.02 × 10(-6), 8.12 × 10(-6), and 1.68 × 10(-6)cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2014
- Full Text
- View/download PDF
16. New monoterpene lactones from Actaea cimicifuga.
- Author
-
Ma LJ, Wang YH, Tang GH, Wang YL, Ma C, Dastmalchi K, Kennelly EJ, and Long CL
- Subjects
- Drug Evaluation, Preclinical methods, Enzyme Inhibitors chemistry, Lactones isolation & purification, Magnetic Resonance Spectroscopy, Medicine, Chinese Traditional, Molecular Structure, Monoterpenes isolation & purification, Plant Roots chemistry, Rhizome chemistry, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Actaea chemistry, Enzyme Inhibitors pharmacology, Lactones chemistry, Lactones pharmacology, Lipase antagonists & inhibitors, Monoterpenes chemistry, Monoterpenes pharmacology
- Abstract
Three new monoterpene lactones, cimicifugolides A-C (1-3), along with a known one (4), were identified from the dried rhizome of Actaea cimicifuga L. that was used as traditional Chinese medicine for thousands of years with the Chinese common name of shengma. The structures of the new isolates were established using spectroscopic methods, including NMR, mass, UV, and IR spectra. The inhibition activity of compounds 1, 2, and 4 against pancreatic lipase was evaluated., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2013
- Full Text
- View/download PDF
17. Bioactive isoquinoline alkaloids from Corydalis saxicola.
- Author
-
Huang QQ, Bi JL, Sun QY, Yang FM, Wang YH, Tang GH, Zhao FW, Wang H, Xu JJ, Kennelly EJ, Long CL, and Yin GF
- Subjects
- Acetylcholinesterase metabolism, Animals, Antiviral Agents chemistry, Antiviral Agents isolation & purification, Antiviral Agents pharmacology, Berberine Alkaloids chemistry, Berberine Alkaloids isolation & purification, Cholinesterase Inhibitors chemistry, Cholinesterase Inhibitors isolation & purification, Dogs, Molecular Structure, Plant Extracts chemistry, Structure-Activity Relationship, Berberine Alkaloids pharmacology, Cholinesterase Inhibitors pharmacology, Corydalis chemistry, Parvovirus drug effects, Plant Extracts pharmacology
- Abstract
Twelve isoquinoline alkaloids including two new nitro-containing tetrahydroprotoberberines, (-)-2,9-dihydroxyl-3,11-dimethoxy-1,10-dinitrotetrahydroprotoberberine (1) and (+)-4-nitroisoapocavidine (2), were isolated from the whole plant of Corydalis saxicola Bunting. The structures of the new compounds were established by spectroscopic analysis and chemical evidence. The inhibitory activity of these isolates against cholinesterase and canine parvovirus were evaluated. Compounds 1 and 1A, (+)-1-nitroapocavidine (5), berberine (8), palmatine (9), dehydrocavidine (10), and sanguinarine (11) showed potent inhibitory activity against acetylcholinesterase with IC(50) values of less than 10 µM, while only compound 1 possessed weak activity against canine parvovirus. Structure-activity studies demonstrated that the nitro substituents at ring A in the tetrahydroprotoberberines led to an increase in the anti-acetylcholinesterase activity., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2012
- Full Text
- View/download PDF
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