8 results on '"Kennelly, Edward J."'
Search Results
2. Anti-inflammatory Constituents from Caulis Trachelospermi#.
- Author
-
Song, Hongzhi, Tan, Jinni, Ma, Ruijing, Kennelly, Edward J, and Tan, Qingang
- Subjects
LIPOPOLYSACCHARIDES ,INTERLEUKINS ,ANTI-inflammatory agents ,ANIMAL experimentation ,WESTERN immunoblotting ,MASS spectrometry ,TUMOR necrosis factors ,PLANT extracts ,NITRIC oxide ,MOLECULAR structure ,ANALYTICAL chemistry ,PHARMACODYNAMICS - Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1 – 3) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22 , and 23 inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23 inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach.
- Author
-
Dake Zhao, Yana Shi, Xinyan Zhu, Li Liu, Pengzhang Ji, Chunlin Long, Yong Shen, and Kennelly, Edward J.
- Subjects
ACONITE ,ANALGESICS ,BIOMARKERS ,DRUG toxicity ,FACTOR analysis ,HERBAL medicine ,CHINESE medicine ,MOLECULAR structure ,QUALITY control ,RESEARCH funding ,IN vitro studies ,METABOLOMICS - Abstract
Despite their well-known toxicity, Aconitum species are important traditional medicines worldwide. Aconitum carmichaelii, known in Chinese as 附子 (fuzi), is an officially recognized traditional Chinese medicine with characteristic analgesic and anti-inflammatory activities, whose principal pharmacological ingredients are considered as aconitine-type diterpene alkaloids. Notwithstanding the long-recorded use of A. carmichaelii in traditional Chinese medicine, no single-entity aconitum alkaloid drug has been developed for clinical use. UPLC-Q-TOF-MS was used to investigate the marker compounds that can be used to differentiate A. carmichaelii from seven other Aconitum species collected in Yunnan Province. Nontargeted principle component analysis scores plots found that all the tested Aconitum species clustered into three distinct groups, and A. carmichaelii was significantly different chemically than the other seven species. Furthermore, the primary and lateral roots of A. carmichaelii also showed significant differences. Using orthogonal partial least squares discriminate analysis analysis, eight marker compounds were identified, including 14-acetylkarakoline, aconitine, carmichaeline, fuziline, hypaconitine, mesaconitine, neoline, and talatisamine. Four of these aconitum alkaloids, fuziline, hypaconitine, mesaconitine, and neoline, showed significant analgesic activity in a dose-dependent manner compared to the negative and positive controls. However, hypaconitine, mesaconitine, and neoline exhibited significant acute toxicity activity, while fuziline showed no acute toxicity in mice, suggesting the relative safety of this alkaloid. This study provides a good example of how to differentiate an authentic medicinal plant from common adulterants using a metabolomics approach, and to identify compounds that may be developed into new drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
4. Transport in Caco-2 Cell Monolayers of Antidiabetic Cucurbitane Triterpenoids from Momordica charantia Fruits.
- Author
-
Shi-Biao Wu, Yue, Grace G. L., Ming-Ho To, Keller, Amy C., Lau, Clara B. S., and Kennelly, Edward J.
- Subjects
DIET therapy for diabetes ,CELL culture ,FRUIT ,RESEARCH methodology ,MEDICINAL plants - Abstract
Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditionalmedicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cellmonolayers, andwere identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the baso-lateral sidewith apparent permeability coefficient (P
app ) values for 3-β-7-β,25-trihydroxycucurbita 5,23(E)-dien-19-al and momordicines I and II at 9.02 x 10-6 ,8.12 x 10-6 , and 1.68 x 10-6 cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
5. Anti-inflammatory Constituents from Caulis Trachelospermi#.
- Author
-
Song, Hongzhi, Tan, Jinni, Ma, Ruijing, Kennelly, Edward J, and Tan, Qingang
- Subjects
- *
LIPOPOLYSACCHARIDES , *INTERLEUKINS , *ANTI-inflammatory agents , *ANIMAL experimentation , *WESTERN immunoblotting , *MASS spectrometry , *TUMOR necrosis factors , *PLANT extracts , *NITRIC oxide , *MOLECULAR structure , *ANALYTICAL chemistry , *PHARMACODYNAMICS - Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1 – 3) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22 , and 23 inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23 inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
6. Anti-inflammatory Constituents from Caulis Trachelospermi.
- Author
-
Song H, Tan J, Ma R, Kennelly EJ, and Tan Q
- Subjects
- Animals, Inflammation drug therapy, Lipopolysaccharides, Macrophages metabolism, Mice, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, RAW 264.7 Cells, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Apocynaceae chemistry, Plant Extracts chemistry, Plant Extracts pharmacology
- Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1: -3: ) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22: , and 23: inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23: inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides ., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
7. Identification of Potential Biomarkers from Aconitum carmichaelii, a Traditional Chinese Medicine, Using a Metabolomic Approach.
- Author
-
Zhao D, Shi Y, Zhu X, Liu L, Ji P, Long C, Shen Y, and Kennelly EJ
- Subjects
- Aconitum toxicity, Analgesics pharmacology, Animals, Biomarkers analysis, Chromatography, High Pressure Liquid methods, Dose-Response Relationship, Drug, Female, Male, Metabolomics methods, Mice, Tandem Mass Spectrometry methods, Aconitum chemistry, Medicine, Chinese Traditional methods
- Abstract
Despite their well-known toxicity, Aconitum species are important traditional medicines worldwide. Aconitum carmichaelii , known in Chinese as (fuzi), is an officially recognized traditional Chinese medicine with characteristic analgesic and anti-inflammatory activities, whose principal pharmacological ingredients are considered as aconitine-type diterpene alkaloids. Notwithstanding the long-recorded use of A. carmichaelii in traditional Chinese medicine, no single-entity aconitum alkaloid drug has been developed for clinical use. UPLC-Q-TOF-MS was used to investigate the marker compounds that can be used to differentiate A. carmichaelii from seven other Aconitum species collected in Yunnan Province. Nontargeted principle component analysis scores plots found that all the tested Aconitum species clustered into three distinct groups, and A. carmichaelii was significantly different chemically than the other seven species. Furthermore, the primary and lateral roots of A. carmichaelii also showed significant differences. Using orthogonal partial least squares discriminate analysis analysis, eight marker compounds were identified, including 14-acetylkarakoline, aconitine, carmichaeline, fuziline, hypaconitine, mesaconitine, neoline, and talatisamine. Four of these aconitum alkaloids, fuziline, hypaconitine, mesaconitine, and neoline, showed significant analgesic activity in a dose-dependent manner compared to the negative and positive controls. However, hypaconitine, mesaconitine, and neoline exhibited significant acute toxicity activity, while fuziline showed no acute toxicity in mice, suggesting the relative safety of this alkaloid. This study provides a good example of how to differentiate an authentic medicinal plant from common adulterants using a metabolomics approach, and to identify compounds that may be developed into new drugs., Competing Interests: The authors declare no conflict of interest., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2018
- Full Text
- View/download PDF
8. Transport in Caco-2 cell monolayers of antidiabetic cucurbitane triterpenoids from Momordica charantia fruits.
- Author
-
Wu SB, Yue GG, To MH, Keller AC, Lau CB, and Kennelly EJ
- Subjects
- Biological Transport, Caco-2 Cells, Chromatography, High Pressure Liquid, Fruit chemistry, Glycosides chemistry, Glycosides isolation & purification, Humans, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Mass Spectrometry, Plants, Medicinal, Sterols chemistry, Sterols isolation & purification, Sterols metabolism, Triterpenes chemistry, Triterpenes isolation & purification, Diabetes Mellitus drug therapy, Glycosides metabolism, Hypoglycemic Agents metabolism, Momordica charantia chemistry, Triterpenes metabolism
- Abstract
Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditional medicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cell monolayers, and were identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the basolateral side with apparent permeability coefficient (P app) values for 3-β-7-β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicines I and II at 9.02 × 10(-6), 8.12 × 10(-6), and 1.68 × 10(-6)cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.