30 results on '"Zhu, Chen"'
Search Results
2. Association between skull bone mineral density and periodontitis: Using the National Health and Nutrition Examination Survey (2011-2014).
- Author
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Fuqian Jin, Jukun Song, Yi Luo, Beichuan Wang, Ming Ding, Jiaxin Hu, and Zhu Chen
- Subjects
Medicine ,Science - Abstract
Background and objectiveBone mineral density (BMD) and periodontitis have been the subject of many studies. However, the relationship between skull (including mandible) BMD and periodontitis has not been extensively studied. An objective of this cross-sectional study was to examine the relationship between skull BMD and periodontitis using data from the National Health and Nutrition Examination Surveys (NHANES) for 2011-2012 and 2013-2014.Materials and methodsFrom NHANES 2011-2014, 3802 participants aged 30-59 were selected. We divided the skull BMD level into quartiles to check the distribution of variables. Periodontitis was defined by the Centers for Disease Control and Prevention (CDC) and the American Association of Periodontology (AAP) in 2012. Multivariate logical regression analysis was used to explore the independent relationship between skull BMD and periodontitis. The generalized additive model (GAM), smooth curve fitting (penalty spline) and threshold effect analysis was used to evaluate dose-response relationship between skull BMD and periodontitis and the potential nonlinear relationship between skull BMD and periodontitis. Finally, subgroup analysis and interaction test were conducted to determine the role of covariates between skull BMD and periodontitis.ResultsThe overall average skull BMD of 3802 participants was 2.24g/cm2, the average age was 43.94 years, and the prevalence of periodontitis was 41.03%. In the fully adjusted logistic regression model, skull BMD and periodontitis showed an independent negative correlation (OR 0.73, 95% CI 0.59-0.90, P = 0.0032) and a linear relationship. Compared with the lowest quartile array (Q1:1.22-1.98) of skull BMD, the highest quartile array(Q4: 2.47-3.79) had a significantly lower risk of periodontitis (OR 0.70,95% CI 0.56-0.87, P = 0.0014). Subgroup analysis showed a highly consistent negative correlation between skull BMD and periodontitis. In the interaction test, people with moderate poverty income ratio (1.57-3.62) and those who had more than 12 alcohol drinks in the past year had a lower risk of periodontitis.ConclusionsThis result suggested that periodontal disease can be related to low skull BMD, for those people, oral hygiene and health care should be more closely monitored. Validation of our findings will require further research.
- Published
- 2022
- Full Text
- View/download PDF
3. Grey relational analysis method for typhoon vulnerability assessment of civil engineering structures based on the 2-tuple linguistic neutrosophic number
- Author
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Qi, Yong, primary, Zhu, Chen, additional, Wang, Fang, additional, and Xia, Yu, additional
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- 2022
- Full Text
- View/download PDF
4. Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy.
- Author
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Yuhan Ma, Qianqian Zhang, Qifu Zhang, Huaqi He, Zhu Chen, Yan Zhao, Da Wei, Mingguang Kong, and Qing Huang
- Subjects
Medicine ,Science - Abstract
As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.
- Published
- 2018
- Full Text
- View/download PDF
5. Xuezhikang contributes to greater triglyceride reduction than simvastatin in hypertriglyceridemia rats by up-regulating apolipoprotein A5 via the PPARα signaling pathway.
- Author
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Shui-Ping Zhao, Rong Li, Wen Dai, Bi-Lian Yu, Lu-Zhu Chen, and Xian-Sheng Huang
- Subjects
Medicine ,Science - Abstract
Xuezhikang (XZK), an extract of Chinese red yeast rice, is recommended as an optimal choice for patients with coronary heart disease (CHD) with markedly elevated triglyceride (TG) levels. This study was designed to compare the hypotriglyceridemic effects between XZK and simvastatin. The role of apolipoprotein A5 (apoA5), a key regulator of TG metabolism and a target gene of peroxisome proliferator-activated receptor α (PPARα), was to be identified in XZK-related hypotriglyceridemic actions. For these goals, hypertriglyceridemia of rats was induced by a high-fructose diet. In order to investigate the hypotriglyceridemic effects of XZK and simvastatin on these animals based on an equivalent low-density lipoprotein cholesterol (LDL-C) lowering power, we titrated their doses (XZK 80 mg/kg/d versus simvastatin 1 mg/kg/d) according to plasma LDL-C reduction of rats. Similarly, we titrated the target doses of the two agents (XZK 500 μg/ml versus simvastatin 10 μM) according to hepatocyte LDL receptor expressions, and then compared the effects of the two agents on TG and apoA5 of hepatocytes in vitro. Our results showed that XZK (80 mg/kg/d) had higher hypotriglyceridemic performance than simvastatin (1 mg/kg/d) on these animals albeit their equivalent LDL-C lowering power. Higher plasma apoA5 levels and hepatic apoA5 expressions were observed in rats treated with XZK (80 mg/kg/d) than simvastatin (1 mg/kg/d). Further, XZK (80 mg/kg/d) contributed to higher hepatic PPARα expressions of rats than simvastatin (1 mg/kg/d). Although the two agents led to an equivalent up-regulation of LDL receptors of hepatocytes, more TG reduction and apoA5 elevation were detected in hepatocytes treated with XZK (500 μg/ml) than simvastatin (10 μM). However, PPARα knockdown eliminated the above effects of XZK on hepatocytes. Therefore, our study indicates that XZK has greater hypotriglyceridemic performance than simvastatin in the setting of an equivalent LDL-C lowering power, which is attributed to more apoA5 up-regulation by this agent via the PPARα signaling pathway.
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- 2017
- Full Text
- View/download PDF
6. Increased yield of AP-3 by inactivation of asm25 in Actinosynnema pretiosum ssp. auranticum ATCC 31565
- Author
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Cheng, Hong, primary, Xiong, Guoqing, additional, Li, Yi, additional, Zhu, Jiaqi, additional, Xiong, Xianghua, additional, Wang, Qingyang, additional, Zhang, Liancheng, additional, Dong, Haolong, additional, Zhu, Chen, additional, Liu, Gang, additional, and Chen, Huipeng, additional
- Published
- 2022
- Full Text
- View/download PDF
7. Inflammation Activation Contributes to Adipokine Imbalance in Patients with Acute Coronary Syndrome.
- Author
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Rong Li, Lu-zhu Chen, Shui-ping Zhao, and Xian-sheng Huang
- Subjects
Medicine ,Science - Abstract
Inflammation can be activated as a defensive response by the attack of acute coronary syndrome (ACS) for ischemic tissue injury. The aim of the present study was to investigate the impact of ACS-activated inflammation on adipokine imbalance and the effects of statins on the crosstalk between inflammation and adipokine imbalance during ACS. In this study, 586 subjects were categorized into: (1) control group; (2) SA (stable angina) group; and (3) ACS group. Circulating levels of hs-CRP, adiponectin and resistin were measured by ELISA. Furthermore, forty C57BL/6 mice were randomized into: sham, AMI, low-statin (atorvastatin, 2 mg/kg/day) and high-statin (atorvastatin, 20 mg/kg/day) group. After 3 weeks, AMI models were established by surgical coronary artery ligation. Circulating levels and adipose expressions of adiponectin and resistin were assessed in animals. Besides, we investigate the effects of atorvastatin on ox-LDL-induced adipokine imbalance in vitro. As a result, we found that ACS patients had higher hs-CRP and resistin levels and lower adiponectin levels. Our correlation analysis demonstrated hs-CRP concentrations were positively correlated with resistin but negatively with adiponectin levels in humans. Our animal findings indicated higher circulating hs-CRP and resistin levels and lower adiponectin levels in AMI mice. Atorvastatin pre-treatment dose-dependently decreased hs-CRP and resistin levels but increased adiponectin levels in mice. The consistent findings were observed about the adipose expressions of resistin and adiponectin in mice. In study in vitro, ox-LDL increased cellular resistin expressions and otherwise for adiponectin expressions, which dose-dependently reversed by the addition of atorvastatin. Therefore, our study indicates that the ACS attack activates inflammation leading to adipokine imbalance that can be ameliorated by anti-inflammation of atorvastatin.
- Published
- 2016
- Full Text
- View/download PDF
8. The Lipoxygenase Gene Family in Poplar: Identification, Classification, and Expression in Response to MeJA Treatment.
- Author
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Zhu Chen, Xue Chen, Hanwei Yan, Weiwei Li, Yuan Li, Ronghao Cai, and Yan Xiang
- Subjects
Medicine ,Science - Abstract
Lipoxygenases (LOXs) are important dioxygenases in cellular organisms. LOXs contribute to plant developmental processes and environmental responses. However, a systematic and comprehensive analysis has not been focused on the LOX gene family in poplar. Therefore, in the present study, we performed a comprehensive analysis of the LOX gene family in poplar.Using bioinformatics methods, we identified a total of 20 LOX genes. These LOX genes were clustered into two subfamilies. The gene structure and motif composition of each subfamily were relatively conserved. These genes are distributed unevenly across nine chromosomes. The PtLOX gene family appears to have expanded due to high tandem and low segmental duplication events. Microarray analysis showed that a number of PtLOX genes have different expression pattern across disparate tissues and under various stress treatments. Quantitative real-time PCR (qRT-PCR) analysis was further performed to confirm the responses to MeJA treatment of the 20 poplar LOX genes. The results show that the PtLOX genes are regulated by MeJA (Methyl jasmonate) treatment.This study provides a systematic analysis of LOX genes in poplar. The gene family analysis reported here will be useful for conducting future functional genomics studies to uncover the roles of LOX genes in poplar growth and development.
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- 2015
- Full Text
- View/download PDF
9. Shifts in Abundance and Diversity of Soil Ammonia-Oxidizing Bacteria and Archaea Associated with Land Restoration in a Semi-Arid Ecosystem.
- Author
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Zhu Chen, Wenliang Wu, Xiaoming Shao, Li Li, Yanbin Guo, and Guochun Ding
- Subjects
Medicine ,Science - Abstract
The Grain to Green Project (GGP) is an unprecedented land restoration action in China. The project converted large areas (ca 10 million ha) of steep-sloped/degraded farmland and barren land into forest and grassland resulting in ecological benefits such as a reduction in severe soil erosion. It may also affect soil microorganisms involved in ammonia oxidization, which is a key step in the global nitrogen cycle. The methods for restoration that are typically adopted in semi-arid regions include abandoning farmland and growing drought tolerant grass (Lolium perenne L.) or shrubs (Caragana korshinskii Kom.). In the present study, the effects of these methods on the abundance and diversity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were evaluated via quantitative real-time PCR, terminal restriction fragment length polymorphism and clone library analysis of amoA genes. Comparisons were made between soil samples from three restored lands and the adjacent farmland in Inner Mongolia. Both the abundance and community composition of AOB were significantly different between the restored lands and the adjacent control. Significantly lower nitrification activity was observed for the restored land. Clone library analysis revealed that all AOB amoA gene sequences were affiliated with Nitrosospira. Abundance of the populations that were associated with Nitrosospira sp. Nv6 which had possibly adapted to high concentrations of inorganic nitrogen, decreased on the restored land. Only a slight difference in the AOB communities was observed between the restored land with and without the shrub (Caragana korshinskii Kom.). A minor effect of land restoration on AOA was observed. In summary, land restoration negatively affected the abundance of AOB and soil nitrification activities, suggesting the potential role of GGP in the leaching of nitrates, and in the emission of N2O in related terrestrial ecosystems.
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- 2015
- Full Text
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10. Risk factors for 3-month mortality in bedridden patients with hospital-acquired pneumonia: A multicentre prospective study
- Author
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Jiao, Jing, primary, Li, Zhen, additional, Wu, Xinjuan, additional, Cao, Jing, additional, Liu, Ge, additional, Liu, Ying, additional, Li, Fangfang, additional, Zhu, Chen, additional, Song, Baoyun, additional, Jin, Jingfen, additional, Liu, Yilan, additional, Wen, Xianxiu, additional, Cheng, Shouzhen, additional, and Wan, Xia, additional
- Published
- 2021
- Full Text
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11. Evaluation and diagnosis of obstacles to land-based ecological security in resource-based cities: A case study of Xingtai city
- Author
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Zhu, Chen, primary, Li, Yuping, additional, Zhang, Luxuan, additional, and Wang, Yanchao, additional
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- 2020
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12. Development and validation a nomogram for predicting the risk of severe COVID-19: A multi-center study in Sichuan, China
- Author
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Rong Yao, Zhu Chen, Yiwu Zhou, Yanqi He, Huan Yang, Zongan Liang, Ting Wang, and He Yu
- Subjects
Male ,Pulmonology ,Physiology ,Fevers ,Blood Pressure ,030204 cardiovascular system & hematology ,Cardiovascular Medicine ,Logistic regression ,Pathology and Laboratory Medicine ,Vascular Medicine ,Severity of Illness Index ,Chronic Liver Disease ,0302 clinical medicine ,Risk Factors ,Chronic Kidney Disease ,Medicine and Health Sciences ,Coughing ,Medicine ,030212 general & internal medicine ,Multidisciplinary ,Liver Diseases ,Regression analysis ,Middle Aged ,Cardiovascular Diseases ,Nephrology ,Cohort ,Hypertension ,Female ,Coronavirus Infections ,Research Article ,Adult ,medicine.medical_specialty ,China ,Adolescent ,Science ,Pneumonia, Viral ,Gastroenterology and Hepatology ,03 medical and health sciences ,Betacoronavirus ,Young Adult ,Signs and Symptoms ,Diagnostic Medicine ,Severity of illness ,Humans ,Pandemics ,Aged ,Retrospective Studies ,business.industry ,SARS-CoV-2 ,Biology and Life Sciences ,COVID-19 ,Retrospective cohort study ,Nomogram ,medicine.disease ,Confidence interval ,Nomograms ,Dyspnea ,Logistic Models ,Emergency medicine ,Respiratory Infections ,Chronic liver disease ,Respiratory infections ,Cardiovascular diseases ,Chronic kidney disease ,business ,Physiological Processes ,Kidney disease - Abstract
BackgroundSince December 2019, coronavirus disease 2019 (COVID-19) emerged in Wuhan and spread across the globe. The objective of this study is to build and validate a practical nomogram for estimating the risk of severe COVID-19.MethodsA cohort of 366 patients with laboratory-confirmed COVID-19 was used to develop a prediction model using data collected from 47 locations in Sichuan province from January 2020 to February 2020. The primary outcome was the development of severe COVID-19 during hospitalization. The least absolute shrinkage and selection operator (LASSO) regression model was used to reduce data size and select relevant features. Multivariable logistic regression analysis was applied to build a prediction model incorporating the selected features. The performance of the nomogram regarding the C-index, calibration, discrimination, and clinical usefulness was assessed. Internal validation was assessed by bootstrapping.ResultsThe median age of the cohort was 43 years. Severe patients were older than mild patients by a median of 6 years. Fever, cough, and dyspnea were more common in severe patients. The individualized prediction nomogram included seven predictors: body temperature at admission, cough, dyspnea, hypertension, cardiovascular disease, chronic liver disease, and chronic kidney disease. The model had good discrimination with an area under the curve of 0.862, C-index of 0.863 (95% confidence interval, 0.801-0.925), and good calibration. A high C-index value of 0.839 was reached in the interval validation. Decision curve analysis showed that the prediction nomogram was clinically useful.ConclusionWe established an early warning model incorporating clinical characteristics that could be quickly obtained on admission. This model can be used to help predict severe COVID-19 and identify patients at risk of developing severe disease.
- Published
- 2020
13. What is creating the height premium? New evidence from a Mendelian randomization analysis in China
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Wang, Jun, primary, Chen, Qihui, additional, Chen, Gang, additional, Li, Yingxiang, additional, Kong, Guoshu, additional, and Zhu, Chen, additional
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- 2020
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14. Factors associated with death in bedridden patients in China: A longitudinal study
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Cao, Jing, primary, Wang, Tiantian, additional, Li, Zhen, additional, Liu, Ge, additional, Liu, Ying, additional, Zhu, Chen, additional, Jiao, Jing, additional, Li, Jiaqian, additional, Li, Fangfang, additional, Liu, Hongpeng, additional, Liu, Huaping, additional, Song, Baoyun, additional, Jin, Jingfen, additional, Liu, Yilan, additional, Wen, Xianxiu, additional, Cheng, Shouzhen, additional, Wan, Xia, additional, and Wu, Xinjuan, additional
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- 2020
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15. Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)
- Author
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Zhou, Chengzhe, primary, Zhu, Chen, additional, Fu, Haifeng, additional, Li, Xiaozhen, additional, Chen, Lan, additional, Lin, Yuling, additional, Lai, Zhongxiong, additional, and Guo, Yuqiong, additional
- Published
- 2019
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16. Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy
- Author
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Qing Huang, Qianqian Zhang, Da Wei, Ma Yuhan, Zhu Chen, He Huaqi, Mingguang Kong, Zhao Yan, and Qifu Zhang
- Subjects
0106 biological sciences ,0301 basic medicine ,Fungal Structure ,Plasma Gases ,Spectrophotometry, Infrared ,Ganoderma ,Molecular biology ,Glycobiology ,Mutagenesis (molecular biology technique) ,lcsh:Medicine ,Infrared Spectroscopy ,Mycology ,Polysaccharide ,Research and Analysis Methods ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,Spectrum Analysis Techniques ,Polysaccharides ,010608 biotechnology ,Genetics ,Food science ,Biomass ,lcsh:Science ,Mycelium ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Strain (chemistry) ,lcsh:R ,Biology and Life Sciences ,near-Infrared Spectroscopy ,Absorption Spectroscopy ,Protoplast ,biology.organism_classification ,RAPD ,Random Amplified Polymorphic DNA Technique ,Mutant Strains ,Artificial gene amplification and extension ,030104 developmental biology ,Molecular biology techniques ,chemistry ,Mutagenesis ,Mutation ,Fermentation ,lcsh:Q ,Research Article - Abstract
As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.
- Published
- 2018
17. Heated humidification did not improve compliance of positive airway pressure and subjective daytime sleepiness in obstructive sleep apnea syndrome: A meta-analysis
- Author
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Zhu, Ding, primary, Wu, Mindan, additional, Cao, Yuan, additional, Lin, Shihua, additional, Xuan, Nanxia, additional, Zhu, Chen, additional, Li, Wen, additional, and Shen, Huahao, additional
- Published
- 2018
- Full Text
- View/download PDF
18. Inflammation Activation Contributes to Adipokine Imbalance in Patients with Acute Coronary Syndrome
- Author
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Shui-ping Zhao, Xiansheng Huang, Rong Li, and Lu-zhu Chen
- Subjects
Male ,0301 basic medicine ,Physiology ,Peptide Hormones ,Atorvastatin ,Myocardial Infarction ,Adipose tissue ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Pathology and Laboratory Medicine ,Biochemistry ,White Blood Cells ,Mice ,0302 clinical medicine ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Adipocytes ,Medicine ,Resistin ,lcsh:Science ,Immune Response ,Connective Tissue Cells ,Innate Immune System ,Multidisciplinary ,Drugs ,Middle Aged ,C-Reactive Protein ,Connective Tissue ,Cytokines ,Female ,Adiponectin ,Cellular Types ,Anatomy ,medicine.symptom ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,medicine.drug ,Acute coronary syndrome ,medicine.medical_specialty ,Inflammatory Diseases ,Immune Cells ,Immunology ,Blotting, Western ,Adipokine ,Inflammation ,03 medical and health sciences ,Signs and Symptoms ,Adipokines ,Internal medicine ,Animals ,Humans ,In patient ,cardiovascular diseases ,Acute Coronary Syndrome ,Pharmacology ,Blood Cells ,business.industry ,Macrophages ,lcsh:R ,Statins ,Biology and Life Sciences ,nutritional and metabolic diseases ,Cell Biology ,Molecular Development ,medicine.disease ,Hormones ,Mice, Inbred C57BL ,Biological Tissue ,030104 developmental biology ,Endocrinology ,Immune System ,lcsh:Q ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,business ,Developmental Biology - Abstract
Inflammation can be activated as a defensive response by the attack of acute coronary syndrome (ACS) for ischemic tissue injury. The aim of the present study was to investigate the impact of ACS-activated inflammation on adipokine imbalance and the effects of statins on the crosstalk between inflammation and adipokine imbalance during ACS. In this study, 586 subjects were categorized into: (1) control group; (2) SA (stable angina) group; and (3) ACS group. Circulating levels of hs-CRP, adiponectin and resistin were measured by ELISA. Furthermore, forty C57BL/6 mice were randomized into: sham, AMI, low-statin (atorvastatin, 2 mg/kg/day) and high-statin (atorvastatin, 20 mg/kg/day) group. After 3 weeks, AMI models were established by surgical coronary artery ligation. Circulating levels and adipose expressions of adiponectin and resistin were assessed in animals. Besides, we investigate the effects of atorvastatin on ox-LDL-induced adipokine imbalance in vitro. As a result, we found that ACS patients had higher hs-CRP and resistin levels and lower adiponectin levels. Our correlation analysis demonstrated hs-CRP concentrations were positively correlated with resistin but negatively with adiponectin levels in humans. Our animal findings indicated higher circulating hs-CRP and resistin levels and lower adiponectin levels in AMI mice. Atorvastatin pre-treatment dose-dependently decreased hs-CRP and resistin levels but increased adiponectin levels in mice. The consistent findings were observed about the adipose expressions of resistin and adiponectin in mice. In study in vitro, ox-LDL increased cellular resistin expressions and otherwise for adiponectin expressions, which dose-dependently reversed by the addition of atorvastatin. Therefore, our study indicates that the ACS attack activates inflammation leading to adipokine imbalance that can be ameliorated by anti-inflammation of atorvastatin.
- Published
- 2016
19. Xuezhikang contributes to greater triglyceride reduction than simvastatin in hypertriglyceridemia rats by up-regulating apolipoprotein A5 via the PPARα signaling pathway
- Author
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Rong Li, Bi-lian Yu, Wen Dai, Lu-zhu Chen, Xiansheng Huang, and Shui-ping Zhao
- Subjects
Male ,0301 basic medicine ,Simvastatin ,Physiology ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Fructoses ,Biochemistry ,Vascular Medicine ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Animal Cells ,Blood plasma ,Medicine and Health Sciences ,Coronary Heart Disease ,lcsh:Science ,Receptor ,Cells, Cultured ,Hypertriglyceridemia ,Multidisciplinary ,Organic Compounds ,Anticholesteremic Agents ,Monosaccharides ,Drugs ,Lipids ,Blood proteins ,Body Fluids ,Chemistry ,Blood ,Liver ,Physical Sciences ,lipids (amino acids, peptides, and proteins) ,Cellular Types ,Anatomy ,Research Article ,medicine.drug ,medicine.medical_specialty ,Lipoproteins ,Carbohydrates ,Cardiology ,Blood Plasma ,03 medical and health sciences ,Internal medicine ,medicine ,Red yeast rice ,Animals ,PPAR alpha ,Triglycerides ,Pharmacology ,Plasma Proteins ,Triglyceride ,Organic Chemistry ,lcsh:R ,Statins ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Cell Biology ,Lipid Metabolism ,medicine.disease ,Rats ,Apolipoproteins ,030104 developmental biology ,Endocrinology ,Gene Expression Regulation ,chemistry ,Apolipoprotein A-V ,LDL receptor ,Hepatocytes ,lcsh:Q ,Drugs, Chinese Herbal - Abstract
Xuezhikang (XZK), an extract of Chinese red yeast rice, is recommended as an optimal choice for patients with coronary heart disease (CHD) with markedly elevated triglyceride (TG) levels. This study was designed to compare the hypotriglyceridemic effects between XZK and simvastatin. The role of apolipoprotein A5 (apoA5), a key regulator of TG metabolism and a target gene of peroxisome proliferator-activated receptor α (PPARα), was to be identified in XZK-related hypotriglyceridemic actions. For these goals, hypertriglyceridemia of rats was induced by a high-fructose diet. In order to investigate the hypotriglyceridemic effects of XZK and simvastatin on these animals based on an equivalent low-density lipoprotein cholesterol (LDL-C) lowering power, we titrated their doses (XZK 80 mg/kg/d versus simvastatin 1 mg/kg/d) according to plasma LDL-C reduction of rats. Similarly, we titrated the target doses of the two agents (XZK 500 μg/ml versus simvastatin 10 μM) according to hepatocyte LDL receptor expressions, and then compared the effects of the two agents on TG and apoA5 of hepatocytes in vitro. Our results showed that XZK (80 mg/kg/d) had higher hypotriglyceridemic performance than simvastatin (1 mg/kg/d) on these animals albeit their equivalent LDL-C lowering power. Higher plasma apoA5 levels and hepatic apoA5 expressions were observed in rats treated with XZK (80 mg/kg/d) than simvastatin (1 mg/kg/d). Further, XZK (80 mg/kg/d) contributed to higher hepatic PPARα expressions of rats than simvastatin (1 mg/kg/d). Although the two agents led to an equivalent up-regulation of LDL receptors of hepatocytes, more TG reduction and apoA5 elevation were detected in hepatocytes treated with XZK (500 μg/ml) than simvastatin (10 μM). However, PPARα knockdown eliminated the above effects of XZK on hepatocytes. Therefore, our study indicates that XZK has greater hypotriglyceridemic performance than simvastatin in the setting of an equivalent LDL-C lowering power, which is attributed to more apoA5 up-regulation by this agent via the PPARα signaling pathway.
- Published
- 2017
20. The Lipoxygenase Gene Family in Poplar: Identification, Classification, and Expression in Response to MeJA Treatment
- Author
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Zhu Chen, Ronghao Cai, Yan Xiang, Xue Chen, Yuan Li, Wei-Wei Li, and Hanwei Yan
- Subjects
medicine.medical_specialty ,Lipoxygenase ,lcsh:Medicine ,Cyclopentanes ,Biology ,Acetates ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Molecular genetics ,Gene expression ,medicine ,Gene family ,Oxylipins ,lcsh:Science ,Gene ,Phylogeny ,Plant Proteins ,Regulation of gene expression ,Genetics ,Multidisciplinary ,Methyl jasmonate ,integumentary system ,Microarray analysis techniques ,Reverse Transcriptase Polymerase Chain Reaction ,lcsh:R ,fungi ,food and beverages ,Populus ,chemistry ,lcsh:Q ,Functional genomics ,Research Article - Abstract
Background Lipoxygenases (LOXs) are important dioxygenases in cellular organisms. LOXs contribute to plant developmental processes and environmental responses. However, a systematic and comprehensive analysis has not been focused on the LOX gene family in poplar. Therefore, in the present study, we performed a comprehensive analysis of the LOX gene family in poplar. Results Using bioinformatics methods, we identified a total of 20 LOX genes. These LOX genes were clustered into two subfamilies. The gene structure and motif composition of each subfamily were relatively conserved. These genes are distributed unevenly across nine chromosomes. The PtLOX gene family appears to have expanded due to high tandem and low segmental duplication events. Microarray analysis showed that a number of PtLOX genes have different expression pattern across disparate tissues and under various stress treatments. Quantitative real-time PCR (qRT-PCR) analysis was further performed to confirm the responses to MeJA treatment of the 20 poplar LOX genes. The results show that the PtLOX genes are regulated by MeJA (Methyl jasmonate) treatment. Conclusions This study provides a systematic analysis of LOX genes in poplar. The gene family analysis reported here will be useful for conducting future functional genomics studies to uncover the roles of LOX genes in poplar growth and development.
- Published
- 2014
21. The IQD Gene Family in Soybean: Structure, Phylogeny, Evolution and Expression
- Author
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Yiyi Wang, Lin Feng, Zhu Chen, Xue Chen, Yan Xiang, Yuan Li, and Hui Ma
- Subjects
Plant Phylogenetics ,Plant Evolution ,Arabidopsis ,lcsh:Medicine ,Plant Genomes ,Plant Science ,Plant Genetics ,Genome ,Segmental Duplications, Genomic ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,Gene Duplication ,Plant Genomics ,Cluster Analysis ,lcsh:Science ,Conserved Sequence ,Phylogeny ,Segmental duplication ,Plant Proteins ,Genetics ,Multidisciplinary ,biology ,food and beverages ,Phylogenetics ,Protein Transport ,Multigene Family ,Brachypodium distachyon ,Brachypodium ,Subcellular Fractions ,Research Article ,Biotechnology ,Protein family ,Sequence analysis ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Synteny ,Chromosomes, Plant ,Evolution, Molecular ,Calmodulin ,Gene family ,Evolutionary Systematics ,Gene ,Crop Genetics ,Evolutionary Biology ,Binding Sites ,Gene Expression Profiling ,lcsh:R ,fungi ,Correction ,Biology and Life Sciences ,Molecular Sequence Annotation ,Oryza ,Sequence Analysis, DNA ,biology.organism_classification ,Organismal Evolution ,Gene expression profiling ,lcsh:Q ,Plant Biotechnology ,Soybeans - Abstract
Members of the plant-specific IQ67-domain (IQD) protein family are involved in plant development and the basal defense response. Although systematic characterization of this family has been carried out in Arabidopsis, tomato (Solanum lycopersicum), Brachypodium distachyon and rice (Oryza sativa), systematic analysis and expression profiling of this gene family in soybean (Glycine max) have not previously been reported. In this study, we identified and structurally characterized IQD genes in the soybean genome. A complete set of 67 soybean IQD genes (GmIQD1–67) was identified using Blast search tools, and the genes were clustered into four subfamilies (IQD I–IV) based on phylogeny. These soybean IQD genes are distributed unevenly across all 20 chromosomes, with 30 segmental duplication events, suggesting that segmental duplication has played a major role in the expansion of the soybean IQD gene family. Analysis of the Ka/Ks ratios showed that the duplicated genes of the GmIQD family primarily underwent purifying selection. Microsynteny was detected in most pairs: genes in clade 1–3 might be present in genome regions that were inverted, expanded or contracted after the divergence; most gene pairs in clade 4 showed high conservation with little rearrangement among these gene-residing regions. Of the soybean IQD genes examined, six were most highly expressed in young leaves, six in flowers, one in roots and two in nodules. Our qRT-PCR analysis of 24 soybean IQD III genes confirmed that these genes are regulated by MeJA stress. Our findings present a comprehensive overview of the soybean IQD gene family and provide insights into the evolution of this family. In addition, this work lays a solid foundation for further experiments aimed at determining the biological functions of soybean IQD genes in growth and development.
- Published
- 2014
22. Atg3 Overexpression Enhances Bortezomib-Induced Cell Death in SKM-1 Cell
- Author
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Zhuang, Lin, primary, Ma, Yan, additional, Wang, Qian, additional, Zhang, Jing, additional, Zhu, Chen, additional, Zhang, Lu, additional, and Xu, Xiaoping, additional
- Published
- 2016
- Full Text
- View/download PDF
23. Synergistic Effect and Molecular Mechanism of Homoharringtonine and Bortezomib on SKM-1 Cell Apoptosis
- Author
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Zhang, Jing, primary, Chen, Bobin, additional, Wu, Ting, additional, Wang, Qian, additional, Zhuang, Lin, additional, Zhu, Chen, additional, Fan, Ni, additional, Qing, Wenjiao, additional, Ma, Yan, additional, and Xu, Xiaoping, additional
- Published
- 2015
- Full Text
- View/download PDF
24. TNF-α-308G/A Polymorphism Contributes to Obstructive Sleep Apnea Syndrome Risk: Evidence Based on 10 Case-Control Studies
- Author
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Wu, Yanping, primary, Cao, Chao, additional, Wu, Yinfang, additional, Zhang, Chao, additional, Zhu, Chen, additional, Ying, Songmin, additional, Chen, Zhihua, additional, Shen, Huahao, additional, and Li, Wen, additional
- Published
- 2014
- Full Text
- View/download PDF
25. Left Ventricular Function during Acute High-Altitude Exposure in a Large Group of Healthy Young Chinese Men
- Author
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Ming-Yue Rao, Jiabei Li, Rongsheng Rao, Baida Xu, Xubin Gao, Huijie Li, Jihang Zhang, Lan Huang, Jun Jin, Guo-Zhu Chen, Shiyong Yu, Jie Yu, and Jun Qin
- Subjects
Adult ,Male ,medicine.medical_specialty ,Cardiac output ,Heart Ventricles ,lcsh:Medicine ,Altitude Sickness ,Doppler echocardiography ,Ventricular Function, Left ,Young Adult ,Internal medicine ,Heart rate ,medicine ,Humans ,Ventricular Function ,Cardiac Output ,lcsh:Science ,Altitude sickness ,Echocardiography, Doppler, Pulsed ,Multidisciplinary ,Ejection fraction ,medicine.diagnostic_test ,business.industry ,Altitude ,lcsh:R ,Organ Size ,Stroke volume ,Effects of high altitude on humans ,medicine.disease ,Myocardial Contraction ,Healthy Volunteers ,Blood pressure ,Cardiology ,lcsh:Q ,business ,Research Article - Abstract
Objective The purpose of this study was to observe left ventricular function during acute high-altitude exposure in a large group of healthy young males. Methods A prospective trial was conducted in Szechwan and Tibet from June to August, 2012. By Doppler echocardiography, left ventricular function was examined in 139 healthy young Chinese men at sea level; within 24 hours after arrival in Lhasa, Tibet, at 3700 m; and on day 7 following an ascent to Yangbajing at 4400 m after 7 days of acclimatization at 3700 m. The resting oxygen saturation (SaO2), heart rate (HR) and blood pressure (BP) were also measured at the above mentioned three time points. Results Within 24 hours of arrival at 3700 m, the HR, ejection fraction (EF), fractional shortening (FS), stroke volume (SV), cardiac output (CO), and left ventricular (LV) Tei index were significantly increased, but the LV end-systolic dimension (ESD), end-systolic volume (ESV), SaO2, E/A ratio, and ejection time (ET) were significantly decreased compared to the baseline levels in all subjects. On day 7 at 4400 m, the SV and CO were significantly decreased; the EF and FS Tei were not decreased compared with the values at 3700 m; the HR was further elevated; and the SaO2, ESV, ESD, and ET were further reduced. Additionally, the E/A ratio was significantly increased on day 7 but was still lower than it was at low altitude. Conclusion Upon acute high-altitude exposure, left ventricular systolic function was elevated with increased stroke volume, but diastolic function was decreased in healthy young males. With higher altitude exposure and prolonged acclimatization, the left ventricular systolic function was preserved with reduced stroke volume and improved diastolic function.
- Published
- 2015
26. The DNA Binding Property of PML/RARA but Not the Integrity of PML Nuclear Bodies Is Indispensable for Leukemic Transformation
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Xi Liu, Hao Yuan, Laurent Peres, Saijuan Chen, Zhu Chen, Hugues de The, Jun Zhou, and Jun Zhu
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Acute promyelocytic leukemia ,Oncogene Proteins, Fusion ,Receptors, Retinoic Acid ,viruses ,Cellular differentiation ,Retinoic acid ,lcsh:Medicine ,Tretinoin ,Promyelocytic Leukemia Protein ,Biology ,DNA-binding protein ,Cell Line ,Malignant transformation ,Mice ,chemistry.chemical_compound ,Leukemia, Promyelocytic, Acute ,Medicine and Health Sciences ,medicine ,Animals ,lcsh:Science ,Molecular Biology ,Cells, Cultured ,Genetics ,Multidisciplinary ,Gene Expression Regulation, Leukemic ,Retinoic Acid Receptor alpha ,Tumor Suppressor Proteins ,lcsh:R ,Biology and Life Sciences ,Nuclear Proteins ,Cell Biology ,Hematology ,DNA ,Transfection ,Hematopoietic Stem Cells ,medicine.disease ,Cell biology ,Mice, Inbred C57BL ,Haematopoiesis ,Cell Transformation, Neoplastic ,Oncology ,chemistry ,Retinoic acid receptor alpha ,Mutation ,lcsh:Q ,Research Article ,Transcription Factors - Abstract
PML/RARA is the oncoprotein driving acute promyelocytic leukemia (APL). It suppresses genes expression by recruitment of a number of transcriptional repressors, resulting in differentiation block and malignant transformation of hematopoietic cells. Here, we found that mice primary hematopoietic progenitor cells (HPCs), transduced by DNA-binding-defective PML/RARA mutants, were deficient in colony formation. Further experiments showed that DNA-binding-defective PML/RARA mutants could not repress the transcription of retinoic acid regulated genes. Intriguingly, there were no significant differences of the micro-speckled intracellular distribution between the mutants and wild-type PML/RARA. Some retinoic acid target genes regulated by PML/RARA are involved in not only differentiation block but also hematopoietic cell self-renewal. Altogether, our data demonstrate that direct DNA-binding is essential for PML/RARA to immortalize hematopoietic cells, while disruption of PML-nuclear body does not seem to be a prerequisite for hematopoietic cell transformation.
- Published
- 2014
27. Characteristics and Expression Patterns of the Aldehyde Dehydrogenase (ALDH) Gene Superfamily of Foxtail Millet (Setaria italica L.)
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Zhu, Chen, primary, Ming, Chen, additional, Zhao-shi, Xu, additional, Lian-cheng, Li, additional, Xue-ping, Chen, additional, and You-zhi, Ma, additional
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- 2014
- Full Text
- View/download PDF
28. Characteristics and Expression Patterns of the Aldehyde Dehydrogenase (ALDH) Gene Superfamily of Foxtail Millet (Setaria italica L.).
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Zhu, Chen, Ming, Chen, Zhao-shi, Xu, Lian-cheng, Li, Xue-ping, Chen, and You-zhi, Ma
- Subjects
- *
ALDEHYDE dehydrogenase genetics , *FOXTAIL millet , *ABIOTIC stress , *EFFECT of stress on crops , *LIPID peroxidation (Biology) , *GENETIC code , *ENVIRONMENTAL toxicology - Abstract
Recent genomic sequencing of the foxtail millet, an abiotic, stress-tolerant crop, has provided a great opportunity for novel gene discovery and functional analysis of this popularly-grown grass. However, few stress-mediated gene families have been studied. Aldehyde dehydrogenases (ALDHs) comprise a gene superfamily encoding NAD (P) +-dependent enzymes that play the role of “aldehyde scavengers”, which indirectly detoxify cellular ROS and reduce the effect of lipid peroxidation meditated cellular toxicity under various environmental stresses. In the current paper, we identified a total of 20 ALDH genes in the foxtail millet genome using a homology search and a phylogenetic analysis and grouped them into ten distinct families based on their amino acid sequence identity. Furthermore, evolutionary analysis of foxtail millet reveals that both tandem and segmental duplication contributed significantly to the expansion of its ALDH genes. The exon-intron structures of members of the same family in foxtail millet or the orthologous genes in rice display highly diverse distributions of their exonic and intronic regions. Also, synteny analysis shows that the majority of foxtail millet and rice ALDH gene homologs exist in the syntenic blocks between the two, implying that these ALDH genes arose before the divergence of cereals. Semi-quantitative and real-time quantitative PCR data reveals that a few SiALDH genes are expressed in an organ-specific manner and that the expression of a number of foxtail millet ALDH genes, such as, SiALDH7B1, SiALDH12A1 and SiALDH18B2 are up-regulated by osmotic stress, cold, H2O2, and phytohormone abscisic acid (ABA). Furthermore, the transformation of SiALDH2B2, SiALDH10A2, SiALDH5F1, SiALDH22A1, and SiALDH3E2 into Escherichia coli (E.coli) was able to improve their salt tolerance. Taken together, our results show that genome-wide identification characteristics and expression analyses provide unique opportunities for assessing the functional roles of foxtail millet ALDH genes in stress responses. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
29. Characteristics and Expression Patterns of the Aldehyde Dehydrogenase (ALDH) Gene Superfamily of Foxtail Millet (Setaria italica L.).
- Author
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Zhu, Chen, Ming, Chen, Zhao-shi, Xu, Lian-cheng, Li, Xue-ping, Chen, and You-zhi, Ma
- Subjects
ALDEHYDE dehydrogenase genetics ,FOXTAIL millet ,ABIOTIC stress ,EFFECT of stress on crops ,LIPID peroxidation (Biology) ,GENETIC code ,ENVIRONMENTAL toxicology - Abstract
Recent genomic sequencing of the foxtail millet, an abiotic, stress-tolerant crop, has provided a great opportunity for novel gene discovery and functional analysis of this popularly-grown grass. However, few stress-mediated gene families have been studied. Aldehyde dehydrogenases (ALDHs) comprise a gene superfamily encoding NAD (P)
+ -dependent enzymes that play the role of “aldehyde scavengers”, which indirectly detoxify cellular ROS and reduce the effect of lipid peroxidation meditated cellular toxicity under various environmental stresses. In the current paper, we identified a total of 20 ALDH genes in the foxtail millet genome using a homology search and a phylogenetic analysis and grouped them into ten distinct families based on their amino acid sequence identity. Furthermore, evolutionary analysis of foxtail millet reveals that both tandem and segmental duplication contributed significantly to the expansion of its ALDH genes. The exon-intron structures of members of the same family in foxtail millet or the orthologous genes in rice display highly diverse distributions of their exonic and intronic regions. Also, synteny analysis shows that the majority of foxtail millet and rice ALDH gene homologs exist in the syntenic blocks between the two, implying that these ALDH genes arose before the divergence of cereals. Semi-quantitative and real-time quantitative PCR data reveals that a few SiALDH genes are expressed in an organ-specific manner and that the expression of a number of foxtail millet ALDH genes, such as, SiALDH7B1, SiALDH12A1 and SiALDH18B2 are up-regulated by osmotic stress, cold, H2 O2 , and phytohormone abscisic acid (ABA). Furthermore, the transformation of SiALDH2B2, SiALDH10A2, SiALDH5F1, SiALDH22A1, and SiALDH3E2 into Escherichia coli (E.coli) was able to improve their salt tolerance. Taken together, our results show that genome-wide identification characteristics and expression analyses provide unique opportunities for assessing the functional roles of foxtail millet ALDH genes in stress responses. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
30. Development and validation a nomogram for predicting the risk of severe COVID-19: A multi-center study in Sichuan, China.
- Author
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Yiwu Zhou, Yanqi He, Huan Yang, He Yu, Ting Wang, Zhu Chen, Rong Yao, and Zongan Liang
- Subjects
Medicine ,Science - Abstract
BackgroundSince December 2019, coronavirus disease 2019 (COVID-19) emerged in Wuhan and spread across the globe. The objective of this study is to build and validate a practical nomogram for estimating the risk of severe COVID-19.MethodsA cohort of 366 patients with laboratory-confirmed COVID-19 was used to develop a prediction model using data collected from 47 locations in Sichuan province from January 2020 to February 2020. The primary outcome was the development of severe COVID-19 during hospitalization. The least absolute shrinkage and selection operator (LASSO) regression model was used to reduce data size and select relevant features. Multivariable logistic regression analysis was applied to build a prediction model incorporating the selected features. The performance of the nomogram regarding the C-index, calibration, discrimination, and clinical usefulness was assessed. Internal validation was assessed by bootstrapping.ResultsThe median age of the cohort was 43 years. Severe patients were older than mild patients by a median of 6 years. Fever, cough, and dyspnea were more common in severe patients. The individualized prediction nomogram included seven predictors: body temperature at admission, cough, dyspnea, hypertension, cardiovascular disease, chronic liver disease, and chronic kidney disease. The model had good discrimination with an area under the curve of 0.862, C-index of 0.863 (95% confidence interval, 0.801-0.925), and good calibration. A high C-index value of 0.839 was reached in the interval validation. Decision curve analysis showed that the prediction nomogram was clinically useful.ConclusionWe established an early warning model incorporating clinical characteristics that could be quickly obtained on admission. This model can be used to help predict severe COVID-19 and identify patients at risk of developing severe disease.
- Published
- 2020
- Full Text
- View/download PDF
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