220 results on '"Wu, Chen"'
Search Results
2. Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac.
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Yingying Li, Wu Chen, Yunsheng Wang, Kun Luo, Yue Li, Lianyang Bai, and Feng Luo
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Medicine ,Science - Abstract
Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path.
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- 2017
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3. Liver Lobe Based Multi-Echo Gradient Recalled Echo T2*-Weighted Imaging in Chronic Hepatitis B-Related Cirrhosis: Association with the Presence and Child-Pugh Class of Cirrhosis.
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Dan Wang, Tian-Wu Chen, Xiao-Ming Zhang, Jie Li, Nan-Lin Zeng, Li Li, Yu-Lian Tang, Yu-Cheng Huang, Rui Li, Fan Chen, and Yan-Li Chen
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Medicine ,Science - Abstract
PURPOSE:To investigate whether liver lobe based T2* values measured on gradient recalled echo T2*-weighted imaging are associated with the presence and Child-Pugh class of hepatitis B-related cirrhosis. METHODS:Fifty-six patients with hepatitis B-related cirrhosis and 23 healthy control individuals were enrolled in this study and underwent upper abdominal T2*-weighted magnetic resonance imaging. T2* values of the left lateral lobe (LLL), left medial lobe (LML), right lobe (RL) and caudate lobe (CL) were measured on T2*-weighted imaging. Statistical analyses were performed to determine the association between liver lobe based T2* values and the presence and Child-Pugh class of cirrhosis. RESULTS:The T2* values of the LLL, LML and RL decreased with the progression of cirrhosis from Child-Pugh class A to C (r = -0.231, -0.223, and -0.395, respectively; all P < 0.05), except that of the CL (r = -0.181, P > 0.05). To a certain extent, Mann-Whitney U tests with Bonferroni correction for multigroup comparisons showed that the T2* values of the LLL, LML and RL could distinguish cirrhotic liver from healthy liver (all P < 0.05), whereas the T2* values of the CL could not (P > 0.05). Receiver operating characteristic analysis demonstrated that the T2* value of the RL could best distinguish cirrhosis from healthy liver, with an area under the receiver operating characteristic curve (AUC) of 0.713 among T2* values of the liver lobes, and that only the T2* value of the RL could distinguish Child-Pugh class C from A-B, with an AUC of 0.697 (all P < 0.05). CONCLUSION:The T2* value of the RL can be associated with the presence and Child-Pugh class of hepatitis B-related cirrhosis.
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- 2016
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4. Transporting Cells in Semi-Solid Gel Condition and at Ambient Temperature.
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Junjian Wang, Peng Chen, Jianzhen Xu, June X Zou, Haibin Wang, and Hong-Wu Chen
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Medicine ,Science - Abstract
Mammalian cells including human cancer cells are usually transported in cryovials on dry ice or in a liquid nitrogen vapor shipping vessel between different places at long distance. The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use. In this study, we tested the viability and properties of cells after being preserved or shipped over long distance in Matrigel mixture for different days. Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen. We also sent cells in the Matrigel mixture via FedEx service to different places at ambient temperature. Upon arrival, it was found that over 90% of the cells were viable and grew well after replating. These data collectively suggested that our Matrigel-based method was highly convenient for shipping live cells for long distances in semi-solid gel condition and at ambient temperature.
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- 2015
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5. High Prevalence of the BIM Deletion Polymorphism in Young Female Breast Cancer in an East Asian Country.
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Ching-Hung Lin, Chen-Yang Shen, Jih-Hsiang Lee, Chiun-Sheng Huang, Chih-Hsin Yang, Wen-Hung Kuo, Dwan-Ying Chang, Chia-Ni Hsiung, Kuan-Ting Kuo, Wei-Wu Chen, I-Chun Chen, Pei-Fang Wu, Sung-Hsin Kuo, Chien-Jen Chen, Yen-Shen Lu, and Ann-Lii Cheng
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Medicine ,Science - Abstract
A rapid surge of female breast cancer has been observed in young women in several East Asian countries. The BIM deletion polymorphism, which confers cell resistance to apoptosis, was recently found exclusively in East Asian people with prevalence rate of 12%. We aimed to evaluate the possible role of this genetic alteration in carcinogenesis of breast cancer in East Asians.Female healthy volunteers (n = 307), patients in one consecutive stage I-III breast cancer cohort (n = 692) and one metastatic breast cancer cohort (n = 189) were evaluated. BIM wild-type and deletion alleles were separately genotyped in genomic DNAs.Both cancer cohorts consistently showed inverse associations between the BIM deletion polymorphism and patient age (≤35 y vs. 36-50 y vs. >50 y: 29% vs. 22% vs. 15%, P = 0.006 in the consecutive cohort, and 40% vs. 23% vs. 13%, P = 0.023 in the metastatic cohort). In healthy volunteers, the frequencies of the BIM deletion polymorphism were similar (13%-14%) in all age groups. Further analyses indicated that the BIM deletion polymorphism was not associated with specific clinicopathologic features, but it was associated with poor overall survival (adjusted hazard ratio 1.71) in the consecutive cohort.BIM deletion polymorphism may be involved in the tumorigenesis of the early-onset breast cancer among East Asians.
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- 2015
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6. Spatiotemporal analysis and the characteristics of the case transmission network of 2019 novel coronavirus disease (COVID-19) in Zhejiang Province, China
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Wu, Haocheng, primary, Wu, Chen, additional, Lu, Qinbao, additional, Ding, Zheyuan, additional, Xue, Ming, additional, and Lin, Junfen, additional
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- 2021
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7. Abdominal regional fat distribution on MRI correlates with cholecystolithiasis.
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Yang Zhang, Tian Wu Chen, Xiao Ming Zhang, Yi-Xiang Wang, Xiao Xiao Chi, Xing Hui Li, Xiao Feng Gao, and Yi Fan Ji
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Medicine ,Science - Abstract
AIMS: To determine whether abdominal regional fat distribution pattern on MRI is correlated with cholecystolithiasis. METHODS: Magnetic resonance imaging (MRI) of 163 patients with cholecystolithiasis and 163 non-cholecystolithiasis control subjects admitted to our institution between March 2011 and September 2013 were included in this cross-sectional evaluation. There were 98 women and 65 men in cholecystolithiasis group with an average age of 57±16 years (range 25-86 years). There were 87 women and 76 men in the control group with an average age of 41±16 years (range 14-77 years). Visceral adipose tissue (VAT), abdominal subcutaneous adipose tissue (SAT) and total abdominal adipose tissue (TAT) of all the subjects at navel level were measured on abdominal MRI. According to the visceral adipose area (cut-off point VAT = 100 cm2), study subjects were divided into 1) increased accumulation of intra-abdominal fat and 2) normal distribution of intra-abdominal fat. Logistic regression was used to assess the association of fat with the presence of cholecystolithiasis, adjusted for age and sex. RESULTS: The incidence of increased intra-abdominal fat accumulation in the cholecystolithiasis group was significantly higher than that of the control group (P = 0.000). After adjusting for age and sex, cholecystolithiasis was associated with a one standard deviation increment in the waist circumference (WC) (OR = 1.44; 95%CI: 1.01,1.93; p = 0.00), VAT (OR = 4.26; 95%CI: 1.85,5.29; p = 0.00), VAT/SAT (OR = 8.66; 95%CI: 1.60,12.63; p = 0.00), and VAT/TAT (OR = 6.73; 95%CI: 4.24,12.18; p = 0.00), but not with fat content in the abdominal subcutaneous fat (p = 0.19). CONCLUSIONS: The visceral adipose tissue and distribution proportion of abdominal adipose tissue are correlates of cholecystolithiasis.
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- 2014
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8. Prognostic and clinicopathological significance of downregulated E-cadherin expression in patients with non-small cell lung cancer (NSCLC): a meta-analysis.
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Yan-Long Yang, Ming-Wu Chen, and Lei Xian
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Medicine ,Science - Abstract
Many studies have investigated the prognostic role of E-cadherin in patients with NSCLC; however, the result still remains inconclusive. An up-to data system review and meta-analysis was necessary to give a comprehensive evaluation of prognostic role of E-cadherin in NSCLC.Eligible studies were searched in Pubmed, Embase and Web of Science databases. The inclusion criteria were studies that assessed the relationship between E-cadherin expression detected by immunohistochemistry (IHC) and the prognosis or clinicopathological features in patients with NSCLC. Subgroup analysis according to race, percentage of reduced/negative E-cadherin expression, histological type, and sample size were also conducted. Odds ratio (OR) or hazard ratio (HR) with 95% confidence interval (CI) were calculated to examine the risk or hazard association.A total of 29 studies including 4010 patients were qualified for analysis. The analysis suggested that downregulated E-cadherin expression was significant associated with unfavorable overall survival (OS) and disease-free survival/progression-free survival (DFS/PFS) in patients with NSCLC. Subgroup analysis by race, percentage of reduced/negative E-cadherin expression, sample size also found the significant association in OS. When only the stage I NSCLC were considered, downregulated E-cadherin expression still had an unfavorable impact on OS. Additionally, downregulated E-cadherin expression was significantly associated with differentiation grade, lymphnode metastasis, vascular invasion, and TNM stage.Downregulated E-cadherin expression detected by IHC seems to correlate with tumour progression and could serve as an important prognostic factor in patients with NSCLC.
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- 2014
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9. The normal transverse mesocolon and involvement of the mesocolon in acute pancreatitis: an MRI study.
- Author
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Xiao Xiao Chi, Xiao Ming Zhang, Tian Wu Chen, Xiao Hua Huang, Lin Yang, Wei Tang, and Bo Xiao
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Medicine ,Science - Abstract
OBJECTIVE: To study the MRI findings of the normal transverse mesocolon and the involvement of the mesocolon in acute pancreatitis (AP) as well as the relationship between the involvement of the mesocolon and the severity of AP. MATERIALS AND METHODS: Forty patients without pancreatic disorders were retrospectively analyzed to observe the normal transverse mesocolon using MRI; 210 patients with AP confirmed by clinical and laboratory tests were retrospectively analyzed using MRI to observe transverse-mesocolon involvement (TMI). The severity of TMI was recorded as zero points (no abnormalities and transverse-mesocolon vessel involvement), one point (linear and patchy signal in the transverse mesocolon) or two points (transverse-mesocolon effusion). The AP severity was graded by the MRI severity index (MRSI) and the Acute Physiology And Chronic Healthy Evaluation II (APACHE II) scoring system. The correlations of TMI with MRSI and APACHE-II were analyzed. RESULTS: In a normal transverse mesocolon, the display rates of the middle colic artery, the middle colic vein and the gastrocolic trunk on MRI were 95.0%, 82.5% and 100.0%, respectively. Of the 210 patients with AP, 130 patients (61.9%) had TMI. According to the TMI grading, 40%, 39% and 20% of the patients were graded at zero, one and two points, respectively. TMI was strongly correlated with the MRSI score (r = 0.759, P = 0.000) and the APACHE-II score (r = 0.384, P = 0.000). CONCLUSION: MRI could be used to visualize transverse-mesocolon involvement. The severity of TMI could reflect that of AP in the clinical setting and imaging. TMI might be a supplementary indicator of the severity of AP.
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- 2014
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10. Quantitative assessment of the presence and severity of cirrhosis in patients with hepatitis B using right liver lobe volume and spleen size measured at magnetic resonance imaging.
- Author
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Xiao-Li Chen, Tian-Wu Chen, Xiao-Ming Zhang, Zhen-Lin Li, Nan-Lin Zeng, Ting Li, Dan Wang, Jie Li, Zhi-Jia Fang, Hang Li, Jia Chen, Jun Liu, Guo-Hui Xu, Jing Ren, Jian-Lin Wu, and Chun-Ping Li
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Medicine ,Science - Abstract
OBJECTIVE: To determine whether right liver lobe volume (RV) and spleen size measured utilizing magnetic resonance (MR) imaging could identify the presence and severity of cirrhosis in patients with hepatitis B. METHODS: Two hundred and five consecutive patients with clinically confirmed diagnosis of cirrhosis due to hepatitis B and 40 healthy control individuals were enrolled in this study and underwent abdominal triphasic enhanced scans using MR imaging. Spleen maximal width (W), thickness (T) and length (L), together with RV and spleen volume (SV), were measured utilizing MR imaging. Spleen multidimensional index (SI) was obtained by multiplying previously acquired parameters W×T×L. Then statistical assessment was performed to evaluate the ability of these parameters, including RV, SV, RV/SV and SI, to identify the presence of cirrhosis and define Child-Pugh class of this disease. RESULTS: SV and SI tended to increase (r = 0.557 and 0.622, respectively; all P
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- 2014
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11. Pancreatic duct patterns in acute pancreatitis: a MRI study.
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Rong Peng, Xiao Ming Zhang, Yi Fan Ji, Tian Wu Chen, Lin Yang, Xiao Hua Huang, and Xiao Xiao Chi
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Medicine ,Science - Abstract
OBJECTIVES: To study the MRI findings of the pancreatic duct in patients with acute pancreatitis. MATERIALS AND METHODS: A total of 239 patients with acute pancreatitis and 125 controls were analyzed in this study. The severity of acute pancreatitis was graded using the MR severity index (MRSI) and the Acute Physiology And Chronic Healthy Evaluation II(APACHE II) scoring systems. The number of main pancreatic duct (MPD) segments visualized, and both MPD diameter and pancreatic duct disruption were noted and compared with the severity of acute pancreatitis. RESULTS: The frequency of MPD segment visualization in the control group was higher than that in the acute pancreatitis group (p0.05). The prevalence of pancreatic duct disruption was 7.9% in the acute pancreatitis group. The prevalences of pancreatic duct disruption were 4.8% and 15.3% in the mild and severe acute pancreatitis groups based on the APACHE II score, respectively, and were 0%, 5.7% and 43.5% in the mild, moderate and severe acute pancreatitis groups according the MRSI score, respectively. The prevalence of pancreatic duct disruption was correlated with the severity of acute pancreatitis based on the APACHE II score (p
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- 2013
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12. Spleen dynamic contrast-enhanced magnetic resonance imaging as a new method for staging liver fibrosis in a piglet model.
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Li Zhou, Tian-wu Chen, Xiao-ming Zhang, Cheng-jun Li, Zhen-feng Yang, Nan-lin Zeng, Li-ying Wang, Ting Li, Dan Wang, Jie Li, Chun-ping Li, Li Li, and Xian-yong Xie
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Medicine ,Science - Abstract
OBJECTIVE: To explore spleen hemodynamic alteration in liver fibrosis with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), and to determine how to stage liver fibrosis with spleen DCE-MRI parameters. MATERIALS AND METHODS: Sixteen piglets were prospectively used to model liver fibrosis staged by liver biopsy, and underwent spleen DCE-MRI on 0, 5th, 9th, 16th and 21st weekend after modeling this disease. DCE-MRI parameters including time to peak (TTP), positive enhancement integral (PEI), maximum slope of increase (MSI) and maximum slope of decrease (MSD) of spleen were measured, and statistically analyzed to stage this disease. RESULTS: Spearman's rank correlation tests showed that TTP tended to increase with increasing stages of liver fibrosis (r = 0.647, P0.05), and decreased from stage 2 to 4 (P0.05). Mann-Whitney tests demonstrated that TTP and PEI could classify fibrosis between stage 0 and 1-4, between 0-1 and 2-4, between 0-2 and 3-4, or between 0-3 and 4 (all P
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- 2013
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13. Liver lobe volumes and the ratios of liver lobe volumes to spleen volume on magnetic resonance imaging for staging liver fibrosis in a minipig model.
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Hang Li, Tian-wu Chen, Xiao-ming Zhang, Zhen-lin Li, Jin-ling Zhang, Dan Wang, Ting Li, Jian-lin Wu, Xing Guo, Xiao-li Chen, Li Li, Xian-yong Xie, and Zi-shu Zhang
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Medicine ,Science - Abstract
OBJECTIVE: To investigate liver lobe volumes and the ratios of liver lobe volumes to spleen volume measured with magnetic resonance imaging (MRI) for quantitatively monitoring and staging liver fibrosis. METHODS: Animal study was approved by Institutional Animal Care and Use Committee. Sixteen minipigs were prospectively used to model liver fibrosis, and underwent abdominal gadolinium-enhanced MRI on 0, 5(th), 9(th), 16(th) and 21(st) weekend after modeling this disease staged by biopsy according to METAVIR classification system. On MRI, volume parameters including left lateral liver lobe volume (LLV), left medial liver lobe volume (LMV), right liver lobe volume (RV), caudate lobe volume (CV), and spleen volume (SV) were measured; and LLV/SV, LMV/SV, RV/SV and CV/SV were calculated. Statistical analyses were performed for staging this fibrosis. RESULTS: LLV and CV increased with increasing stage of fibrosis (r = 0.711, 0.526, respectively; all P < 0.05). RV and LMV increased from stage 0 to 2 and decreased from 2 to 4; and RV/SV decreased from 0 to 1, increased from 1 to 2, and decreased from 3 to 4 (all P > 0.05). LLV/SV, LMV/SV and CV/SV decreased from stage 0 to 4 (r = -0.566, -0.748 and -0.620, respectively; all P < 0.05). LLV, CV, LLV/SV, LMV/SV, RV/SV, and CV/SV could distinguish stage 0-1 from 2-4 and 0-2 from 3-4 (all P < 0.05). Among these parameters, LLV and LMV/SV could best classify stage ≥2 and ≥3, respectively (area under receiver operating characteristic curve = 0.893 and 0.946, respectively). CONCLUSION: LLV and LMV/SV complement each other in staging liver fibrosis, and both parameters should be used to stage this disease.
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- 2013
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14. The prevalence and demographic features of congenital cytomegalovirus infection in an urban area of East Asia: A population-based study
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Yang, Tzong-Hann, primary, Huang, Hung-Meng, additional, Hsu, Wei-Chung, additional, Tsao, Po-Nien, additional, Liu, Tien-Chen, additional, Hsu, Chuan-Jen, additional, Huang, Li-Min, additional, Wu, Chuan-Song, additional, Weng, Shih-Ming, additional, Lu, Chun-Yi, additional, and Wu, Chen-Chi, additional
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- 2021
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15. Altered expression of genes regulating inflammation and synaptogenesis during regrowth of afferent neurons to cochlear hair cells
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Wu, Chen-Chi, primary, Brugeaud, Aurore, additional, Seist, Richard, additional, Lin, Hsiao-Chun, additional, Yeh, Wei-Hsi, additional, Petrillo, Marco, additional, Coppola, Giovanni, additional, Edge, Albert S. B., additional, and Stankovic, Konstantina M., additional
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- 2020
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16. A combination of 7-ketocholesterol, lysosphingomyelin and bile acid-408 to diagnose Niemann-Pick disease type C using LC-MS/MS
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Wu, Chen, primary, Iwamoto, Takeo, additional, Hossain, Mohammad Arif, additional, Akiyama, Keiko, additional, Igarashi, Junko, additional, Miyajima, Takashi, additional, and Eto, Yoshikatsu, additional
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- 2020
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17. Field-based screening of selected oral antibiotics in Belize
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Husaini, Danladi Chiroma, primary, Kishan, Uppala Jyana Venkata, additional, Wu, Chen-Yi, additional, Guerra, Elsbeth Nerissa, additional, Bush, Cindy J., additional, Perez, Ayleen Walewska, additional, and Coc, Israel, additional
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- 2020
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18. The seroconversion rate of QuantiFERON-TB Gold In-Tube test in psoriatic patients receiving secukinumab and ixekizumab, the anti-interleukin-17A monoclonal antibodies
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Wu, Chen-Yu, primary, Chiu, Hsien-Yi, additional, and Tsai, Tsen-Fang, additional
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- 2019
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19. Comparison of the accuracy of diagnoses of oral potentially malignant disorders with dysplasia by a general dental clinician and a specialist using the Taiwanese Nationwide Oral Mucosal Screening Program
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Cheng-Yu Yang, Yuan-Wu Chen, Tien-En Chiang, Yu-Chun Lin, Chi-Tsung Wu, and Sheng-Tang Wu
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Male ,Carcinogenesis ,Dentists ,Oral Mucosal Cancers ,Risk Factors ,Medicine and Health Sciences ,Mass Screening ,Medical diagnosis ,Oral Leukoplakia ,Early Detection of Cancer ,Leukoplakia ,Multidisciplinary ,Histopathological analysis ,Middle Aged ,Head and Neck Tumors ,Predictive value ,Oncology ,Homogeneous ,Medicine ,Female ,Mouth Neoplasms ,Neoplastic Transformation ,Research Article ,Adult ,Dysplasia ,medicine.medical_specialty ,Science ,Taiwan ,Young Adult ,Signs and Symptoms ,Malignant Tumors ,Diagnostic Medicine ,Cancer Detection and Diagnosis ,medicine ,Humans ,Neoplastic transformation ,Aged ,business.industry ,Mouth Mucosa ,Cancers and Neoplasms ,medicine.disease ,Dermatology ,Head and Neck Cancers ,Lesions ,Histopathology ,Clinical Medicine ,Mouth Diseases ,business - Abstract
Screening for oral potentially malignant disorders (OPMDs) with dysplasia in high-risk groups is suggested in countries with a high prevalence of the disorders. This study aimed to compare the accuracy of diagnoses of OPMDs with dysplasia made by a primary examiner (general dental clinician) and a specialist (oral and maxillofacial surgeon) using the current Taiwanese Nationwide Oral Mucosal Screening Program (TNOMSP). A total of 134 high-risk participants were enrolled for oral mucosal screening via the TNOMSP. A primary examiner and a specialist examined each participant. Mucosal biopsies were obtained and subjected to histopathological analysis. The OPMD most frequently diagnosed by the primary examiner was thin homogeneous leukoplakia (48/134; 35.8%), and in 39/134 participants (29.1%) the diagnosis was uncertain, but abnormalities were suggested. The OPMDs most frequently diagnosed by the specialist were erythroleukoplakia (23/134; 17.2%) and thin homogeneous leukoplakia (21/134; 15.7%), and 51/134 participants (38.1%) were diagnosed with other diseases. Via histopathology, 70/134 participants (52.3%) were diagnosed with dysplasia, and 58/134 (43.3%) were diagnosed with benign conditions. The specialist’s diagnoses exhibited a higher specificity, positive predictive value, and accuracy than the primary examiners. A specialist using the current TNOMSP for high-risk participants diagnosed OPMDs with dysplasia more accurately than a primary examiner. Early diagnosis of high-risk OPMDs is crucial in countries with a high prevalence of the disorders. Proficient examination via the current TNOMSP by trained clinician is effective for the management of OPMDs with dysplasia.
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- 2021
20. Downregulation of Jumonji-C domain-containing protein 5 inhibits proliferation by silibinin in the oral cancer PDTX model
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Yin-Lai Wang, Gu-Jiun Lin, Yuan-Wu Chen, Huey-Kang Sytwu, Cheng-Yu Yang, Chih-Kung Lin, Cheng-Chih Hsieh, Wei-Chin Chang, Chang-Huei Tsao, Yu-Hsuan Li, Jen-Chen Cheng, and Chun-Shu Lin
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0301 basic medicine ,Cancer Treatment ,Apoptosis ,Immunostaining ,Mice, SCID ,medicine.disease_cause ,Metastasis ,Mice ,chemistry.chemical_compound ,Prostate cancer ,0302 clinical medicine ,Mice, Inbred NOD ,Basic Cancer Research ,Medicine and Health Sciences ,Tumor Cells, Cultured ,Staining ,Histone Demethylases ,Multidisciplinary ,Tissue microarray ,Cell Staining ,Animal Models ,Prognosis ,Gene Expression Regulation, Neoplastic ,Survival Rate ,Oncology ,Experimental Organism Systems ,Cell Processes ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Medicine ,Female ,Mouth Neoplasms ,Research Article ,Science ,Silibinin ,Mouse Models ,Research and Analysis Methods ,03 medical and health sciences ,Model Organisms ,Downregulation and upregulation ,Diagnostic Medicine ,Biomarkers, Tumor ,medicine ,Animals ,Humans ,Survival rate ,Cell Proliferation ,business.industry ,Biology and Life Sciences ,Cancer ,Cell Biology ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Xenograft Model Antitumor Assays ,Nuclear Staining ,Repressor Proteins ,stomatognathic diseases ,030104 developmental biology ,chemistry ,Specimen Preparation and Treatment ,Silybin ,Animal Studies ,Trans-Activators ,Cancer research ,Carcinogenesis ,business - Abstract
Dysregulation of histone demethylase Jumonji-C domain-containing protein 5 (JMJD5) has been identified as a great effect on tumorigenesis. Silibinin is a commonly used anti-hepatotoxic drug and exhibits anticancer effect in various cancers. However, the antitumor mechanism between silibinin and JMJD5 in oral squamous cell carcinoma (OSCC) remains unclear. In this study, the clinical significance of JMJD5 on OSCC patients was assessed through tissue microarray. Furthermore, mice bearing patient-derived tumor xenografts (PDTXs) and tongue cancer cell lines were treated with silibinin and evaluated for tumor growth and JMJD5 expression. High expression of JMJD5 in oral cancer was significantly associated with tumor size (P = 0.0241), cervical node metastasis (P = 0.0001) and clinical stage (P = 0.0002), was associated with worse survival rate compared with that of the total cohort (P = 0.0002). Collectively the data indicate that JMJD5 expression may be suitable for detection of unfavorable prognosis in OSCC patients, based in part on its apparent role as a marker of metastasis. In addition, silibinin inhibits cancer growth in vitro and in PDTX models. Furthermore, metastasis-associated protein 1 (MTA1) could regulate the expression for JMJD5 and had a positive correlation with JMJD5. Moreover, silibinin could downregulate JMJD5 and MTA1 in oral cancer. Present study thus identifies that JMJD5 might be an essential prognostic indicator and therapeutic target against OSCC progression. In addition, silibinin is a potential candidate among novel chemotherapeutic agents or adjuvants for modulating JMJD5 in OSCC, through a mechanism likely involving MTA1/JMJD5 axis.
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- 2020
21. Evaluating the effects of control interventions and estimating the inapparent infections for dengue outbreak in Hangzhou, China
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Wu, Haocheng, primary, Wu, Chen, additional, Lu, Qinbao, additional, Ding, Zheyuan, additional, Xue, Ming, additional, and Lin, Junfen, additional
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- 2019
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22. Fat-to-muscle ratio is a useful index for cardiometabolic risks: A population-based observational study
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Chen, Yuan-Yuei, primary, Fang, Wen-Hui, additional, Wang, Chung-Ching, additional, Kao, Tung-Wei, additional, Yang, Hui-Fang, additional, Wu, Chen-Jung, additional, Sun, Yu-Shan, additional, Wang, Ying-Chuan, additional, and Chen, Wei-Liang, additional
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- 2019
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23. Helicobacter pylori infection increases risk of incident metabolic syndrome and diabetes: A cohort study
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Chen, Yuan-Yuei, primary, Fang, Wen-Hui, additional, Wang, Chung-Ching, additional, Kao, Tung-Wei, additional, Chang, Yaw-Wen, additional, Wu, Chen-Jung, additional, Zhou, Yi-Chao, additional, Sun, Yu-Shan, additional, and Chen, Wei-Liang, additional
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- 2019
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24. Unique spectra of deafness-associated mutations in Mongolians provide insights into the genetic relationships among Eurasian populations
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Erdenechuluun, Jargalkhuu, primary, Lin, Yin-Hung, additional, Ganbat, Khongorzul, additional, Bataakhuu, Delgermaa, additional, Makhbal, Zaya, additional, Tsai, Cheng-Yu, additional, Lin, Yi-Hsin, additional, Chan, Yen-Hui, additional, Hsu, Chuan-Jen, additional, Hsu, Wei-Chung, additional, Chen, Pei-Lung, additional, and Wu, Chen-Chi, additional
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- 2018
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25. Exploring the link between metabolic syndrome and risk of dysmobility syndrome in elderly population
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Chen, Yuan-Yuei, primary, Kao, Tung-Wei, additional, Wang, Chung-Ching, additional, Chen, Ying-Jen, additional, Wu, Chen-Jung, additional, and Chen, Wei-Liang, additional
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- 2018
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26. Body fat has stronger associations with bone mass density than body mass index in metabolically healthy obesity
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Chen, Yuan-Yuei, primary, Fang, Wen-Hui, additional, Wang, Chung-Ching, additional, Kao, Tung-Wei, additional, Chang, Yaw-Wen, additional, Wu, Chen-Jung, additional, Zhou, Yi-Chao, additional, Sun, Yu-Shan, additional, and Chen, Wei-Liang, additional
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- 2018
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27. Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
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Feng Luo, Yingying Li, Kun Luo, Yunsheng Wang, Lianyang Bai, Wu Chen, and Yue Li
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0301 basic medicine ,Metabolic Processes ,Leaves ,lcsh:Medicine ,Plant Science ,010501 environmental sciences ,Pathology and Laboratory Medicine ,01 natural sciences ,Biochemistry ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Agricultural Soil Science ,Medicine and Health Sciences ,Soil Pollutants ,Quinclorac ,Food science ,lcsh:Science ,Flowering Plants ,Multidisciplinary ,biology ,Strain (chemistry) ,Plant Anatomy ,Temperature ,Eukaryota ,Esters ,Agriculture ,Genomics ,Hydrogen-Ion Concentration ,Plants ,Bacterial Pathogens ,Enzymes ,Actinobacteria ,Chemistry ,Biodegradation, Environmental ,Medical Microbiology ,Physical Sciences ,Quinolines ,Pathogens ,Research Article ,Nicotiana ,Soil Science ,Microbiology ,Mycobacterium ,03 medical and health sciences ,Bioremediation ,Genetics ,Microbial Pathogens ,0105 earth and related environmental sciences ,Bacteria ,Herbicides ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Mycobacteria ,Proteins ,Methyltransferases ,Biodegradation ,biology.organism_classification ,16S ribosomal RNA ,030104 developmental biology ,Metabolism ,chemistry ,Genes, Bacterial ,Fermentation ,Enzymology ,lcsh:Q - Abstract
Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path.
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- 2017
28. Detrimental association between betel nut chewing and colorectal polyps in adult populations
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Chen, Yuan-Yuei, primary, Fang, Wen-Hui, additional, Wang, Chung-Ching, additional, Kao, Tung-Wei, additional, Chang, Yaw-Wen, additional, Yang, Hui-Fang, additional, Wu, Chen-Jung, additional, Sun, Yu-Shan, additional, and Chen, Wei-Liang, additional
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- 2018
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29. Increased body fat percentage in patients with hepatitis B and C virus infection
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Chen, Yuan-Yuei, primary, Fang, Wen-Hui, additional, Wang, Chung-Ching, additional, Kao, Tung-Wei, additional, Chang, Yaw-Wen, additional, Yang, Hui-Fang, additional, Wu, Chen-Jung, additional, Sun, Yu-Shan, additional, and Chen, Wei-Liang, additional
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- 2018
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30. Exploring the association between thyroid- stimulating hormone and metabolic syndrome: A large population-based study
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Zhou, Yi-Chao, primary, Fang, Wen-Hui, additional, Kao, Tung-Wei, additional, Wang, Chung-Ching, additional, Chang, Yaw-Wen, additional, Peng, Tao-Chun, additional, Wu, Chen-Jung, additional, Yang, Hui-Fang, additional, Chan, James Yi-Hsin, additional, and Chen, Wei-Liang, additional
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- 2018
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31. Personal exposure measurements of school-children to fine particulate matter (PM2.5) in winter of 2013, Shanghai, China
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Zhang, Lijun, primary, Guo, Changyi, additional, Jia, Xiaodong, additional, Xu, Huihui, additional, Pan, Meizhu, additional, Xu, Dong, additional, Shen, Xianbiao, additional, Zhang, Jianghua, additional, Tan, Jianguo, additional, Qian, Hailei, additional, Dong, Chunyang, additional, Shi, Yewen, additional, Zhou, Xiaodan, additional, and Wu, Chen, additional
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- 2018
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32. Comorbidity profiles of psoriasis in Taiwan: A latent class analysis
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Wu, Chen-Yi, primary, Hu, Hsiao-Yun, additional, Li, Chung-Pin, additional, Chou, Yiing-Jeng, additional, and Chang, Yun-Ting, additional
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- 2018
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33. Preliminary Study of MR Diffusion Tensor Imaging of Pancreas for the Diagnosis of Acute Pancreatitis
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Emmanuel Ajedichiga Aduah, Tian-wu Chen, Ling Zhuang, Xiao-Ming Zhang, Jian Wang, Jiani Hu, Xing Hui Li, and Liangjun Li
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lcsh:Medicine ,Pathology and Laboratory Medicine ,Biochemistry ,030218 nuclear medicine & medical imaging ,Diagnostic Radiology ,Fats ,0302 clinical medicine ,Medicine and Health Sciences ,lcsh:Science ,Brain Mapping ,Multidisciplinary ,medicine.diagnostic_test ,Radiology and Imaging ,Physics ,Condensed Matter Physics ,Magnetic Resonance Imaging ,Lipids ,medicine.anatomical_structure ,Diffusion Tensor Imaging ,Physical Sciences ,Acute pancreatitis ,Radiology ,Anatomy ,Pancreas ,Research Article ,medicine.medical_specialty ,Imaging Techniques ,Brain Morphometry ,Materials Science ,Material Properties ,Neuroimaging ,Endocrine System ,Gastroenterology and Hepatology ,Research and Analysis Methods ,03 medical and health sciences ,Necrosis ,Exocrine Glands ,Signs and Symptoms ,Diagnostic Medicine ,Fractional anisotropy ,medicine ,Effective diffusion coefficient ,Rank correlation ,business.industry ,Diffusion Weighted Imaging ,lcsh:R ,Biology and Life Sciences ,Magnetic resonance imaging ,medicine.disease ,body regions ,Pancreatitis ,Anisotropy ,lcsh:Q ,business ,Nuclear medicine ,030217 neurology & neurosurgery ,Diffusion MRI ,Neuroscience - Abstract
Objectives To evaluate the feasibility of differentiating between acute pancreatitis (AP) and healthy pancreas using diffusion tensor imaging (DTI) and correlate apparent diffusion coefficient (ADC) /fractional anisotropy (FA) values with the severity of AP. Material and Methods 66 patients diagnosed with AP and 20 normal controls (NC) underwent DTI sequences and routine pancreatic MR sequences on a 3.0T MRI scanner. Average ADC and FA values of the pancreatic were measured. Differences of FA and ADC values between the AP group and the NC group with AP and healthy pancreas were compared by two-sample independent t-test. The severity of AP on MRI was classified into subgroups using MR severity index (MRSI), where the mean FA and ADC values were calculated. Relationship among the FA values, ADC values and MRSI were analyzed using Spearman's rank correlation coefficients. Results The pancreatic mean ADC value in the AP group (1.68 ± 0.45×10−3mm2/s) was significantly lower than in the NC group (2.09 ± 0.55×10−3mm2/s) (P = 0.02); the same as mean FA value (0.39 ± 0.23 vs 0.54 ± 0.12, P = 0.00). In the subgroup analysis, the pancreatic ADC and FA value of edema AP patients was significantly higher than necrosis AP patients with P = 0.000 and P = 0.001respectively. In addition, as severity of pancreatitis increased according to MRSI, lower pancreatic ADC (r = -0.635) and FA value (r = -0.654) were noted. Conclusion Both FA and ADC value from DTI can be used to differentiate AP patients from NC. Both ADC and FA value of pancreas have a negative correlation with the severity of AP.
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- 2016
34. A Next-Generation Sequencing Data Analysis Pipeline for Detecting Unknown Pathogens from Mixed Clinical Samples and Revealing Their Genetic Diversity
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Guang-Wu Chen, Shin-Ru Shih, Kuo-Chien Tsao, Ching-Ju Lee, Shu-Li Yang, and Yu-Nong Gong
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0301 basic medicine ,Molecular biology ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Polymerase Chain Reaction ,law.invention ,Madin Darby Canine Kidney Cells ,Database and Informatics Methods ,Sequencing techniques ,law ,DNA sequencing ,lcsh:Science ,Polymerase chain reaction ,Phylogeny ,Genetics ,Recombination, Genetic ,Viral Genomics ,Multidisciplinary ,Phylogenetic tree ,Nucleotide Mapping ,High-Throughput Nucleotide Sequencing ,Phylogenetic Analysis ,Genomics ,Genomic Databases ,Virus Diseases ,Viral evolution ,Viruses ,Transcriptome Analysis ,Sequence Analysis ,Research Article ,Next-Generation Sequencing ,030106 microbiology ,Sequence Databases ,Enzyme-Linked Immunosorbent Assay ,Microbial Genomics ,Genome, Viral ,Biology ,Research and Analysis Methods ,Microbiology ,Cell Line ,03 medical and health sciences ,Dogs ,Gene mapping ,Virology ,Cell Line, Tumor ,Genetic variation ,Animals ,Humans ,Amino Acid Sequence ,Genetic diversity ,Molecular Biology Assays and Analysis Techniques ,lcsh:R ,Gene Mapping ,Biology and Life Sciences ,Computational Biology ,Genetic Variation ,Reproducibility of Results ,Genome Analysis ,030104 developmental biology ,Biological Databases ,Molecular biology techniques ,Microbial genetics ,lcsh:Q - Abstract
Forty-two cytopathic effect (CPE)-positive isolates were collected from 2008 to 2012. All isolates could not be identified for known viral pathogens by routine diagnostic assays. They were pooled into 8 groups of 5-6 isolates to reduce the sequencing cost. Next-generation sequencing (NGS) was conducted for each group of mixed samples, and the proposed data analysis pipeline was used to identify viral pathogens in these mixed samples. Polymerase chain reaction (PCR) or enzyme-linked immunosorbent assay (ELISA) was individually conducted for each of these 42 isolates depending on the predicted viral types in each group. Two isolates remained unknown after these tests. Moreover, iteration mapping was implemented for each of these 2 isolates, and predicted human parechovirus (HPeV) in both. In summary, our NGS pipeline detected the following viruses among the 42 isolates: 29 human rhinoviruses (HRVs), 10 HPeVs, 1 human adenovirus (HAdV), 1 echovirus and 1 rotavirus. We then focused on the 10 identified Taiwanese HPeVs because of their reported clinical significance over HRVs. Their genomes were assembled and their genetic diversity was explored. One novel 6-bp deletion was found in one HPeV-1 virus. In terms of nucleotide heterogeneity, 64 genetic variants were detected from these HPeVs using the mapped NGS reads. Most importantly, a recombination event was found between our HPeV-3 and a known HPeV-4 strain in the database. Similar event was detected in the other HPeV-3 strains in the same clade of the phylogenetic tree. These findings demonstrated that the proposed NGS data analysis pipeline identified unknown viruses from the mixed clinical samples, revealed their genetic identity and variants, and characterized their genetic features in terms of viral evolution.
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- 2016
35. Genomic Signatures for Avian H7N9 Viruses Adapting to Humans
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Kuo-Chien Tsao, Shin-Ru Shih, Guang-Wu Chen, Yu-Nong Gong, Shu-Ming Kuo, Shu-Li Yang, Mei-Ren Hsiao, and Yi-Chun Liu
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RNA viruses ,Models, Molecular ,0301 basic medicine ,Protein Conformation ,viruses ,lcsh:Medicine ,Bird Genomics ,Influenza A Virus, H7N9 Subtype ,medicine.disease_cause ,Biochemistry ,Genome ,Database and Informatics Methods ,Influenza A virus ,lcsh:Science ,Pathology and laboratory medicine ,Ribonucleoprotein ,Genetics ,Viral Genomics ,Mutation ,Mammalian Genomics ,Multidisciplinary ,Genomics ,Medical microbiology ,Genomic Databases ,Ribonucleoproteins ,Viruses ,Vertebrates ,Pathogens ,Research Article ,030106 microbiology ,RNA-dependent RNA polymerase ,Microbial Genomics ,Genome, Viral ,Biology ,Research and Analysis Methods ,Microbiology ,Virus ,Cell Line ,H7N9 ,Birds ,Viral Proteins ,03 medical and health sciences ,Virology ,Influenza, Human ,medicine ,Influenza viruses ,Animals ,Humans ,Position-Specific Scoring Matrices ,Medicine and health sciences ,Biology and life sciences ,lcsh:R ,Organisms ,Viral pathogens ,Proteins ,Computational Biology ,Genetic Variation ,Genome Analysis ,RNA-Dependent RNA Polymerase ,Influenza A virus subtype H5N1 ,Microbial pathogens ,Biological Databases ,030104 developmental biology ,Amino Acid Substitution ,Animal Genomics ,lcsh:Q ,Orthomyxoviruses - Abstract
An avian influenza A H7N9 virus emerged in March 2013 and caused a remarkable number of human fatalities. Genome variability in these viruses may provide insights into host adaptability. We scanned over 140 genomes of the H7N9 viruses isolated from humans and identified 104 positions that exhibited seven or more amino acid substitutions. Approximately half of these substitutions were identified in the influenza ribonucleoprotein (RNP) complex. Although PB2 627K of the avian virus promotes replication in humans, 45 of the 147 investigated PB2 sequences retained the E signature at this position, which is an avian characteristic. We discovered 10 PB2 substitutions that covaried with K627E. An RNP activity assay showed that Q591K, D701N, and M535L restored the polymerase activity in human cells when 627K transformed to an avian-like E. Genomic analysis of the human-isolated avian influenza virus is crucial in assessing genome variability, because relationships between position-specific variations can be observed and explored. In this study, we observed alternative positions that can potentially compensate for PB2 627K, a well-known marker for cross-species infection. An RNP assay suggested Q591K, D701N, and M535L as potential markers for an H7N9 virus capable of infecting humans.
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- 2016
36. Liver Lobe Based Multi-Echo Gradient Recalled Echo T2*-Weighted Imaging in Chronic Hepatitis B-Related Cirrhosis: Association with the Presence and Child-Pugh Class of Cirrhosis
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Li Li, Yu-cheng Huang, Dan Wang, Yan-li Chen, Nan-lin Zeng, Tian-wu Chen, Rui Li, Yu-lian Tang, Fan Chen, Xiao-Ming Zhang, and Jie Li
- Subjects
Liver Cirrhosis ,Male ,Cirrhosis ,Physiology ,lcsh:Medicine ,Pediatrics ,Biochemistry ,Gastroenterology ,Diagnostic Radiology ,030218 nuclear medicine & medical imaging ,0302 clinical medicine ,Blood Flow ,Gradient recalled echo ,Medicine and Health Sciences ,Public and Occupational Health ,lcsh:Science ,Aged, 80 and over ,Observer Variation ,Multidisciplinary ,medicine.diagnostic_test ,Liver Diseases ,Radiology and Imaging ,Child Health ,Hematology ,Middle Aged ,Magnetic Resonance Imaging ,Body Fluids ,Blood ,medicine.anatomical_structure ,Liver ,Liver Fibrosis ,Female ,030211 gastroenterology & hepatology ,Anatomy ,Research Article ,Adult ,medicine.medical_specialty ,Imaging Techniques ,Gastroenterology and Hepatology ,Research and Analysis Methods ,Veins ,03 medical and health sciences ,Hepatitis B, Chronic ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,Hemoglobin ,Portal Veins ,Aged ,Hepatitis ,Receiver operating characteristic ,business.industry ,lcsh:R ,Biology and Life Sciences ,Proteins ,Magnetic resonance imaging ,Blood flow ,medicine.disease ,Lobe ,ROC Curve ,Liver Lobe ,Gastrointestinal Imaging ,Cardiovascular Anatomy ,Blood Vessels ,lcsh:Q ,business ,Liver and Spleen Scan - Abstract
Purpose To investigate whether liver lobe based T2* values measured on gradient recalled echo T2*-weighted imaging are associated with the presence and Child-Pugh class of hepatitis B-related cirrhosis. Methods Fifty-six patients with hepatitis B-related cirrhosis and 23 healthy control individuals were enrolled in this study and underwent upper abdominal T2*-weighted magnetic resonance imaging. T2* values of the left lateral lobe (LLL), left medial lobe (LML), right lobe (RL) and caudate lobe (CL) were measured on T2*-weighted imaging. Statistical analyses were performed to determine the association between liver lobe based T2* values and the presence and Child-Pugh class of cirrhosis. Results The T2* values of the LLL, LML and RL decreased with the progression of cirrhosis from Child-Pugh class A to C (r = -0.231, -0.223, and -0.395, respectively; all P < 0.05), except that of the CL (r = -0.181, P > 0.05). To a certain extent, Mann-Whitney U tests with Bonferroni correction for multigroup comparisons showed that the T2* values of the LLL, LML and RL could distinguish cirrhotic liver from healthy liver (all P < 0.05), whereas the T2* values of the CL could not (P > 0.05). Receiver operating characteristic analysis demonstrated that the T2* value of the RL could best distinguish cirrhosis from healthy liver, with an area under the receiver operating characteristic curve (AUC) of 0.713 among T2* values of the liver lobes, and that only the T2* value of the RL could distinguish Child-Pugh class C from A-B, with an AUC of 0.697 (all P < 0.05). Conclusion The T2* value of the RL can be associated with the presence and Child-Pugh class of hepatitis B-related cirrhosis.
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- 2016
37. Spatial characteristics and the epidemiology of human infections with avian influenza A(H7N9) virus in five waves from 2013 to 2017 in Zhejiang Province, China
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Wu, Haocheng, primary, Wang, XinYi, additional, Xue, Ming, additional, Xue, Melanie, additional, Wu, Chen, additional, Lu, Qinbao, additional, Ding, Zheyuan, additional, Xv, Xiaoping, additional, and Lin, Junfen, additional
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- 2017
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38. Examining the gender difference in the association between metabolic syndrome and the mean leukocyte telomere length
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Cheng, Yuan-Yuei, primary, Kao, Tung-Wei, additional, Chang, Yaw-Wen, additional, Wu, Chen-Jung, additional, Peng, Tao-Chun, additional, Wu, Li-Wei, additional, Yang, Hui-Fang, additional, Liaw, Fang-Yih, additional, and Chen, Wei-Liang, additional
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- 2017
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39. Postprandial Glucose as a Risk Factor for Elevated Intraocular Pressure
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Wu, Chen-Jung, primary, Fang, Wen-Hui, additional, Kao, Tung-Wei, additional, Chen, Ying-Jen, additional, Liaw, Fang-Yih, additional, Chang, Yaw-Wen, additional, Wang, Gia-Chi, additional, Peng, Tao-Chun, additional, and Chen, Wei-Liang, additional
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- 2016
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40. Preliminary Study of MR Diffusion Tensor Imaging of the Liver for the Diagnosis of Hepatocellular Carcinoma
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Xing Hui Li, Tian-wu Chen, Qi Liang, Xiao-Ming Zhang, Jiani Hu, Ling Zhuang, Horea Calimente, Liangjun Li, Yinan Wei, and Jun Liu
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Adult ,Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Biopsy ,lcsh:Medicine ,behavioral disciplines and activities ,Young Adult ,Tensor (intrinsic definition) ,medicine ,Carcinoma ,Image Processing, Computer-Assisted ,Humans ,lcsh:Science ,Aged ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Liver Neoplasms ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,digestive system diseases ,body regions ,Diffusion Tensor Imaging ,nervous system ,Hepatocellular carcinoma ,Case-Control Studies ,lcsh:Q ,Female ,Radiology ,Mr diffusion ,business ,Diffusion MRI ,Research Article - Abstract
Objectives To evaluate the feasibility of differentiating between hepatocellular carcinomas (HCC) and healthy liver using diffusion tensor imaging (DTI). Material and Methods All subjects underwent an abdominal examination on a 3.0T MRI scanner. Two radiologists independently scored the image quality (IQ). An optimal set of DTI parameters was obtained from a group of fifteen volunteers with multiple b-values (100, 300, 500, and 800 s/mm2) and various diffusion-encoding directions (NED = 6, 9, and 12)using two way ANOVA analysis. Eighteen Patients with HCC underwent DTI scans with the optimized parameters. Fractional anisotropy(FA) and average apparent diffusion coefficient (ADC) values were measured. The differences of FA and ADC values between liver healthy region and HCC lesion were compared through paired t tests. Results There were no significant changes in liver IQ and FA/ADC values with increased NED(P >0.05), whereas the liver IQ and FA/ADC values decreased significantly with increased b-values(P
- Published
- 2015
41. High Prevalence of the BIM Deletion Polymorphism in Young Female Breast Cancer in an East Asian Country
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Chiun-Sheng Huang, Ching-Hung Lin, Jih-Hsiang Lee, Yen-Shen Lu, Chia-Ni Hsiung, Chien-Jen Chen, Chih-Hsin Yang, Wen-Hung Kuo, Pei-Fang Wu, Wei-Wu Chen, Kuan-Ting Kuo, Dwan-Ying Chang, I-Chun Chen, Ann-Lii Cheng, Sung-Hsin Kuo, and Chen-Yang Shen
- Subjects
Oncology ,Adult ,medicine.medical_specialty ,Prevalence ,lcsh:Medicine ,Breast Neoplasms ,Biology ,medicine.disease_cause ,Cell resistance ,Young Adult ,Breast cancer ,Asian People ,Internal medicine ,Proto-Oncogene Proteins ,medicine ,Humans ,East Asia ,Genetic Predisposition to Disease ,Neoplasm Metastasis ,Young female ,lcsh:Science ,Aged ,Neoplasm Staging ,Sequence Deletion ,Aged, 80 and over ,Multidisciplinary ,High prevalence ,Polymorphism, Genetic ,Bcl-2-Like Protein 11 ,Asia, Eastern ,lcsh:R ,Age Factors ,Membrane Proteins ,Middle Aged ,medicine.disease ,Prognosis ,Human genetics ,Case-Control Studies ,Immunology ,lcsh:Q ,Female ,Neoplasm Grading ,Carcinogenesis ,Apoptosis Regulatory Proteins ,Research Article - Abstract
Background A rapid surge of female breast cancer has been observed in young women in several East Asian countries. The BIM deletion polymorphism, which confers cell resistance to apoptosis, was recently found exclusively in East Asian people with prevalence rate of 12%. We aimed to evaluate the possible role of this genetic alteration in carcinogenesis of breast cancer in East Asians. Method Female healthy volunteers (n = 307), patients in one consecutive stage I-III breast cancer cohort (n = 692) and one metastatic breast cancer cohort (n = 189) were evaluated. BIM wild-type and deletion alleles were separately genotyped in genomic DNAs. Results Both cancer cohorts consistently showed inverse associations between the BIM deletion polymorphism and patient age (≤35 y vs. 36-50 y vs. >50 y: 29% vs. 22% vs. 15%, P = 0.006 in the consecutive cohort, and 40% vs. 23% vs. 13%, P = 0.023 in the metastatic cohort). In healthy volunteers, the frequencies of the BIM deletion polymorphism were similar (13%-14%) in all age groups. Further analyses indicated that the BIM deletion polymorphism was not associated with specific clinicopathologic features, but it was associated with poor overall survival (adjusted hazard ratio 1.71) in the consecutive cohort. Conclusions BIM deletion polymorphism may be involved in the tumorigenesis of the early-onset breast cancer among East Asians.
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- 2015
42. The Use of Solitaire AB Stents in Coil Embolization of Wide-Necked Cerebral Aneurysms
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Ming-Ke Wang, Li-Ping Wei, Shu-Kai Wang, Lei Yan, Tai-Min Song, Yuan-Hong He, Xinwei Han, Ji Ma, Dong Guo, Hong-Can Zhu, Wen-Wu Chen, Teng-Fei Li, and Shaofeng Shui
- Subjects
Male ,medicine.medical_specialty ,Solitaire Cryptographic Algorithm ,medicine.medical_treatment ,lcsh:Medicine ,Neurosurgical Procedures ,Aneurysm ,Blood vessel prosthesis ,medicine ,Humans ,Postoperative Period ,Embolization ,cardiovascular diseases ,lcsh:Science ,Interventional neuroradiology ,Retrospective Studies ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Stent ,Intracranial Aneurysm ,Digital subtraction angiography ,Middle Aged ,medicine.disease ,equipment and supplies ,Embolization, Therapeutic ,Blood Vessel Prosthesis ,Cerebral Angiography ,Treatment Outcome ,surgical procedures, operative ,Female ,Stents ,lcsh:Q ,Radiology ,business ,Follow-Up Studies ,Research Article ,Cerebral angiography - Abstract
Background The Solitaire AB stent is one of many assistant stents used for treating wide-necked cerebral aneurysm, and has been used since 2003. However, large sample studies on its safety and effectiveness are lacking. The objective of this study was to evaluate the effectiveness and safety of the Solitaire AB stent in the coil embolization of wide-necked cerebral aneurysms. Methods Retrospective review of the clinical and image data of 116 patients with wide-necked cerebral aneurysms who had been enrolled at six interventional neuroradiology centers from February 2010 to February 2014 and had been treated by coil embolization; in total, 120 Solitaire AB stents were used. The degree of aneurysm occlusion was examined using digital subtraction angiography (DSA) immediately after the procedure and during follow-up, and was graded using the modified Raymond classification. We also observed complications to evaluate the safety and effectiveness of this therapy. Results The 120 Solitaire AB stents (4 mm × 15 mm, four stents; 4 mm × 20 mm, 16 stents; 6 mm × 20 mm, 36 stents; 6 mm × 30 mm, 64 stents) were inserted to treat 120 wide-necked cerebral aneurysms. All stents were inserted successfully. DSA immediately post-surgery revealed 55 cases of complete occlusion, 59 cases of neck remnant, and six cases of aneurysm remnant. Perioperatively, there were four cases of hemorrhage and four cases of stent thrombosis. The follow-up spanned 3–37 months; of 92 patients examined by DSA at the 6-month follow up, 12 had disease recurrence. Conclusions The Solitaire AB stent is effective with a good technical success rate and short-term effect for assisting coil embolization of wide-necked cerebral aneurysms.
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- 2015
43. Prognostic and clinicopathological significance of downregulated E-cadherin expression in patients with non-small cell lung cancer (NSCLC): a meta-analysis
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Ming-Wu Chen, Lei Xian, and Yan-Long Yang
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Oncology ,medicine.medical_specialty ,Lung Neoplasms ,Cancer Treatment ,lcsh:Medicine ,non-small cell lung cancer (NSCLC) ,Down-Regulation ,Subgroup analysis ,Metastasis ,Predictive Value of Tests ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Basic Cancer Research ,medicine ,Medicine and Health Sciences ,Humans ,Stage (cooking) ,lcsh:Science ,Survival analysis ,Multidisciplinary ,business.industry ,lcsh:R ,Hazard ratio ,Odds ratio ,medicine.disease ,Cadherins ,Prognosis ,Survival Analysis ,Meta-analysis ,lcsh:Q ,business ,Biomarkers ,Research Article - Abstract
BACKGROUND Many studies have investigated the prognostic role of E-cadherin in patients with NSCLC; however, the result still remains inconclusive. An up-to data system review and meta-analysis was necessary to give a comprehensive evaluation of prognostic role of E-cadherin in NSCLC. METHODS Eligible studies were searched in Pubmed, Embase and Web of Science databases. The inclusion criteria were studies that assessed the relationship between E-cadherin expression detected by immunohistochemistry (IHC) and the prognosis or clinicopathological features in patients with NSCLC. Subgroup analysis according to race, percentage of reduced/negative E-cadherin expression, histological type, and sample size were also conducted. Odds ratio (OR) or hazard ratio (HR) with 95% confidence interval (CI) were calculated to examine the risk or hazard association. RESULTS A total of 29 studies including 4010 patients were qualified for analysis. The analysis suggested that downregulated E-cadherin expression was significant associated with unfavorable overall survival (OS) and disease-free survival/progression-free survival (DFS/PFS) in patients with NSCLC. Subgroup analysis by race, percentage of reduced/negative E-cadherin expression, sample size also found the significant association in OS. When only the stage I NSCLC were considered, downregulated E-cadherin expression still had an unfavorable impact on OS. Additionally, downregulated E-cadherin expression was significantly associated with differentiation grade, lymphnode metastasis, vascular invasion, and TNM stage. CONCLUSION Downregulated E-cadherin expression detected by IHC seems to correlate with tumour progression and could serve as an important prognostic factor in patients with NSCLC.
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- 2014
44. A novel compound NSC745885 exerts an anti-tumor effect on tongue cancer SAS cells in vitro and in vivo
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Yi Shing Shieh, Huey-Kang Sytwu, Hsu Shan Huang, Dueng-Yuan Hueng, Kuo-Hsing Ma, Shing Hwa Huang, Yuan Wu Chen, and Gu-Jiun Lin
- Subjects
Cancer Treatment ,lcsh:Medicine ,Anthraquinones ,Apoptosis ,Mice, SCID ,Pharmacology ,Mice ,Oral Diseases ,Mice, Inbred NOD ,Drug Discovery ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,Caspase 3 ,Tongue Neoplasms ,Oncology ,Carcinoma, Squamous Cell ,Mouth Neoplasms ,medicine.drug ,Research Article ,Drug Research and Development ,Oral Medicine ,Antineoplastic Agents ,X-Linked Inhibitor of Apoptosis Protein ,Tongue ,In vivo ,Cell Line, Tumor ,Thiadiazoles ,medicine ,Carcinoma ,Animals ,Humans ,Doxorubicin ,RNA, Messenger ,Cell Proliferation ,Cardiotoxicity ,business.industry ,Cell growth ,lcsh:R ,Cancer ,medicine.disease ,Xenograft Model Antitumor Assays ,Squamous carcinoma ,stomatognathic diseases ,Dentistry ,Cancer cell ,lcsh:Q ,Neoplasm Recurrence, Local ,Clinical Medicine ,business - Abstract
Objective Oral squamous cell carcinoma (OSCC) is a prevalent cancer, especially in developing countries. Anthracyclines and their anthraquinone derivatives, such as doxorubicin, exhibit a cell growth inhibitory effect and have been used as anti-cancer drugs for many years. However, the cardiotoxicity of anthracycline antibiotics is a major concern in their clinical application. NSC745885 is a novel compound synthesized from 1,2-diaminoanthraquinone, which subsequently reacts with thionyl chloride and triethylamine. The present study aimed to investigate the anti-oral cancer potential and the safety of NSC745885. Methods We investigated the anti-cancer potential of NSC745885 in oral squamous carcinoma cell lines and in an in vivo oral cancer xenograft mouse model. The expression of apoptotic related genes were evaluated by real-time RT-PCR and western bloting, and the in vivo assessment of apoptotic marker were measured by immunohistochemical staining. The anti-tumor efficiency and safety between doxorubicin and NSC745885 were also compared. Results Our results demonstrated that NSC745885 exhibits anti-oral cancer activity through the induction of apoptosis in cancer cells and in tumor-bearing mice, and this treatment did not induce marked toxicity in experimental mice. This compound also exhibits a comparable anti-tumor efficiency and a higher safety in experimental mice when compared to doxorubicin. Conclusions The data of this study provide evidence for NSC745885 as a potential novel therapeutic drug for the treatment of human OSCC.
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- 2014
45. Quantitative assessment of the presence and severity of cirrhosis in patients with hepatitis B using right liver lobe volume and spleen size measured at magnetic resonance imaging
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Jianlin Wu, Dan Wang, Jia Chen, Zhi-jia Fang, Hang Li, Jun Liu, Xiao-Ming Zhang, Nan-lin Zeng, Tian-wu Chen, Jie Li, Xiao-li Chen, Guo-hui Xu, Ting Li, Chun-ping Li, Zhen-Lin Li, and Jing Ren
- Subjects
Liver Cirrhosis ,Male ,Pathology ,Cirrhosis ,Epidemiology ,Gastroenterology and hepatology ,lcsh:Medicine ,Gastroenterology ,Diagnostic Radiology ,Medicine ,Clinical Epidemiology ,lcsh:Science ,Aged, 80 and over ,Observer Variation ,Multidisciplinary ,medicine.diagnostic_test ,Organ Size ,Hepatitis B ,Middle Aged ,Magnetic Resonance Imaging ,Healthy Volunteers ,Infectious hepatitis ,medicine.anatomical_structure ,Liver ,Female ,Radiology ,Research Article ,Adult ,medicine.medical_specialty ,Clinical Research Design ,Spleen ,Diagnostic Medicine ,Internal medicine ,Humans ,In patient ,Liver diseases ,Aged ,Right liver lobe ,Receiver operating characteristic ,business.industry ,lcsh:R ,Case-control study ,Magnetic resonance imaging ,medicine.disease ,ROC Curve ,Case-Control Studies ,lcsh:Q ,business - Abstract
Objective To determine whether right liver lobe volume (RV) and spleen size measured utilizing magnetic resonance (MR) imaging could identify the presence and severity of cirrhosis in patients with hepatitis B. Methods Two hundred and five consecutive patients with clinically confirmed diagnosis of cirrhosis due to hepatitis B and 40 healthy control individuals were enrolled in this study and underwent abdominal triphasic enhanced scans using MR imaging. Spleen maximal width (W), thickness (T) and length (L), together with RV and spleen volume (SV), were measured utilizing MR imaging. Spleen multidimensional index (SI) was obtained by multiplying previously acquired parameters W×T×L. Then statistical assessment was performed to evaluate the ability of these parameters, including RV, SV, RV/SV and SI, to identify the presence of cirrhosis and define Child-Pugh class of this disease. Results SV and SI tended to increase (r = 0.557 and 0.622, respectively; all P
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- 2013
46. Spatial Variations of the Methanogenic Communities in the Sediments of Tropical Mangroves
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Jing, Hongmei, primary, Cheung, Shunyan, additional, Zhou, Zhi, additional, Wu, Chen, additional, Nagarajan, Sanjay, additional, and Liu, Hongbin, additional
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- 2016
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47. Does Pay-For-Performance Program Increase Providers Adherence to Guidelines for Managing Hepatitis B and Hepatitis C Virus Infection in Taiwan?
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Chen, Huei-Ju, primary, Huang, Nicole, additional, Chen, Long-Sheng, additional, Chou, Yiing-Jenq, additional, Li, Chung-Pin, additional, Wu, Chen-Yi, additional, and Chang, Yu-Chia, additional
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- 2016
- Full Text
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48. De Novo Transcriptome Analysis of the Common New Zealand Stick Insect Clitarchus hookeri (Phasmatodea) Reveals Genes Involved in Olfaction, Digestion and Sexual Reproduction
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Wu, Chen, primary, Crowhurst, Ross N., additional, Dennis, Alice B., additional, Twort, Victoria G., additional, Liu, Shanlin, additional, Newcomb, Richard D., additional, Ross, Howard A., additional, and Buckley, Thomas R., additional
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- 2016
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49. Spatial Dynamics of the Communities and the Role of Major Countries in the International Rare Earths Trade: A Complex Network Analysis
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Wang, Xibo, primary, Ge, Jianping, additional, Wei, Wendong, additional, Li, Hanshi, additional, Wu, Chen, additional, and Zhu, Ge, additional
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- 2016
- Full Text
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50. Liver lobe volumes and the ratios of liver lobe volumes to spleen volume on magnetic resonance imaging for staging liver fibrosis in a minipig model
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Jianlin Wu, Li Li, Ting Li, Zhen-lin Li, Xing Guo, Xiao-li Chen, Jin-ling Zhang, Hang Li, Zishu Zhang, Dan Wang, Xiao-Ming Zhang, Xian-yong Xie, and Tian-wu Chen
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Liver Cirrhosis ,Male ,Pathology ,medicine.medical_specialty ,Swine ,Liver fibrosis ,lcsh:Medicine ,Spleen ,Fibrosis ,Biopsy ,medicine ,Animals ,Stage (cooking) ,lcsh:Science ,Observer Variation ,Multidisciplinary ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,lcsh:R ,Magnetic resonance imaging ,Organ Size ,medicine.disease ,Magnetic Resonance Imaging ,Disease Models, Animal ,medicine.anatomical_structure ,Liver Lobe ,Liver ,ROC Curve ,Swine, Miniature ,Female ,lcsh:Q ,business ,Research Article - Abstract
OBJECTIVE: To investigate liver lobe volumes and the ratios of liver lobe volumes to spleen volume measured with magnetic resonance imaging (MRI) for quantitatively monitoring and staging liver fibrosis. METHODS: Animal study was approved by Institutional Animal Care and Use Committee. Sixteen minipigs were prospectively used to model liver fibrosis, and underwent abdominal gadolinium-enhanced MRI on 0, 5(th), 9(th), 16(th) and 21(st) weekend after modeling this disease staged by biopsy according to METAVIR classification system. On MRI, volume parameters including left lateral liver lobe volume (LLV), left medial liver lobe volume (LMV), right liver lobe volume (RV), caudate lobe volume (CV), and spleen volume (SV) were measured; and LLV/SV, LMV/SV, RV/SV and CV/SV were calculated. Statistical analyses were performed for staging this fibrosis. RESULTS: LLV and CV increased with increasing stage of fibrosis (r = 0.711, 0.526, respectively; all P < 0.05). RV and LMV increased from stage 0 to 2 and decreased from 2 to 4; and RV/SV decreased from 0 to 1, increased from 1 to 2, and decreased from 3 to 4 (all P > 0.05). LLV/SV, LMV/SV and CV/SV decreased from stage 0 to 4 (r = -0.566, -0.748 and -0.620, respectively; all P < 0.05). LLV, CV, LLV/SV, LMV/SV, RV/SV, and CV/SV could distinguish stage 0-1 from 2-4 and 0-2 from 3-4 (all P < 0.05). Among these parameters, LLV and LMV/SV could best classify stage ≥2 and ≥3, respectively (area under receiver operating characteristic curve = 0.893 and 0.946, respectively). CONCLUSION: LLV and LMV/SV complement each other in staging liver fibrosis, and both parameters should be used to stage this disease.
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- 2013
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