1. A super-resolution scanning algorithm for lensless microfluidic imaging using the dual-line array image sensor
- Author
-
Li Na, Zhengpeng Li, Dian Tian, Ningmei Yu, and Shuaijun Li
- Subjects
Computer science ,Entropy ,Microfluidics ,Cell Culture Techniques ,Velocity ,02 engineering and technology ,Lab-On-A-Chip Devices ,Image Processing, Computer-Assisted ,Image resolution ,0303 health sciences ,Microscopy ,Multidisciplinary ,Physics ,Applied Mathematics ,Simulation and Modeling ,Classical Mechanics ,Light Microscopy ,021001 nanoscience & nanotechnology ,Chip ,Tilt (optics) ,Physical Sciences ,Engineering and Technology ,Thermodynamics ,Medicine ,Fluidics ,0210 nano-technology ,Algorithm ,Diffraction ,Algorithms ,Research Article ,Imaging Techniques ,Science ,Acceleration ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Image processing ,Research and Analysis Methods ,03 medical and health sciences ,Motion ,Miniaturization ,Animals ,Humans ,Image sensor ,030304 developmental biology ,Pixel ,Superresolution ,Computer Science::Computer Vision and Pattern Recognition ,Waves ,Mathematics - Abstract
The lensless optical fluid microscopy is of great significance to the miniaturization, portability and low cost development of cell detection instruments. However, the resolution of the cell image collected directly is low, because the physical pixel size of the image sensor is the same order of magnitude as the cell size. To solve this problem, this paper proposes a super-resolution scanning algorithm using a dual-line array sensor and a microfluidic chip. For dual-line array sensor images, the multi-group velocity and acceleration of cells flowing through the line array sensor are calculated. Then the reconstruction model of the super-resolution image is constructed with variable acceleration. By changing the angle between the line array image sensor and the direction of cell flow, the super-resolution image scanning and reconstruction are achieved in both horizontal and vertical directions. In addition, it is necessary to study the row by row extraction algorithm for cell foreground image. In this paper, the dual-line array sensor is implemented by adjusting the acquisition window of the image sensor with a pixel size of 2.2μm. When the tilt angle is 21 degrees, the equivalent pixel size is 0.79μm, improved 2.8 times, and after de-diffraction its average size error was 3.249%. As the angle decreases, the image resolution is higher, but the amount of information is less. This super-resolution scanning algorithm can be integrated on the chip and used with a microfluidic chip to realize on-chip instrument.
- Published
- 2020