1. The protective effect of non-invasive low intensity pulsed electric field and fucoidan in preventing oxidative stress-induced motor neuron death via ROCK/Akt pathway
- Author
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Chih-Hsiung Hsieh, Chueh-Hsuan Lu, Yu-Yi Kuo, Guan-Bo Lin, and Chih-Yu Chao
- Subjects
0301 basic medicine ,Protein Expression ,medicine.disease_cause ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Neurons ,Motor Neurons ,Neuronal Death ,rho-Associated Kinases ,Multidisciplinary ,Cell Death ,Neuronal Morphology ,Fucoidan ,Neurodegeneration ,Neurodegenerative Diseases ,medicine.anatomical_structure ,Cell Processes ,Phosphorylation ,Medicine ,Cellular Types ,Research Article ,Signal Transduction ,Science ,Surgical and Invasive Medical Procedures ,Electric Stimulation Therapy ,Research and Analysis Methods ,Cell Line ,03 medical and health sciences ,Polysaccharides ,Neurites ,Gene Expression and Vector Techniques ,medicine ,Animals ,Humans ,Molecular Biology Techniques ,Molecular Biology ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Molecular Biology Assays and Analysis Techniques ,Functional Electrical Stimulation ,business.industry ,Biology and Life Sciences ,Cell Biology ,Neuronal Dendrites ,Motor neuron ,medicine.disease ,Oxidative Stress ,030104 developmental biology ,chemistry ,Cellular Neuroscience ,Cancer research ,Neuron ,business ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery ,Oxidative stress ,Neuroscience - Abstract
With the expansion of the aged population, it is predicted that neurodegenerative diseases (NDDs) will become a major threat to public health worldwide. However, existing therapies can control the symptoms of the diseases at best, rather than offering a fundamental cure. As for the complex pathogenesis, clinical and preclinical researches have indicated that oxidative stress, a central role in neuronal degeneration, is a possible therapeutic target in the development of novel remedies. In this study, the motor neuron-like cell line NSC-34 was employed as an experimental model in probing the effects induced by the combination of non-invasive low intensity pulsed electric field (LIPEF) and fucoidan on the H2O2-induced neuron damage. It was found that single treatment of the LIPEF could protect the NSC-34 cells from oxidative stress, and the protective effect was enhanced by combining the LIPEF and fucoidan. Notably, it was observed that single treatment of the LIPEF obviously suppressed the H2O2-enhanced expression of ROCK protein and increased the phosphorylation of Akt in the H2O2-treated NSC-34 cells. Moreover, the LIPEF can be easily modified to concentrate on a specific area. Accordingly, this technique can be used as an advanced remedy for ROCK inhibition without the drawback of drug metabolism. Therefore, we suggest the LIPEF would be a promising strategy as a treatment for motor neurodegeneration and warrant further probe into its potential in treating other neuronal degenerations.
- Published
- 2019