1. Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway
- Author
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Guan-Bo Lin, Chueh-Hsuan Lu, Chih-Yu Chao, Yi-Kun Sun, Wei Ting Chen, Yu-Yi Kuo, and Chih-Hsiung Hsieh
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Cell signaling ,Antioxidant ,SH-SY5Y ,medicine.medical_treatment ,Gene Expression ,Apoptosis ,Signal transduction ,ERK signaling cascade ,Alzheimer's Disease ,Biochemistry ,chemistry.chemical_compound ,Medical Conditions ,0302 clinical medicine ,Electricity ,Medicine and Health Sciences ,Enzyme assays ,Colorimetric assays ,Phosphorylation ,Cyclic AMP Response Element-Binding Protein ,Hydrogen peroxide ,Cytotoxicity ,Bioassays and physiological analysis ,Membrane Potential, Mitochondrial ,chemistry.chemical_classification ,rho-Associated Kinases ,Antioxidant Therapy ,MTT assay ,Multidisciplinary ,Cell Death ,Pharmaceutics ,Chemistry ,Kinase ,Physics ,Drugs ,Signaling cascades ,Cell Differentiation ,Neurodegenerative Diseases ,Neuroprotection ,Neurology ,Electric Field ,Cell Processes ,Physical Sciences ,Medicine ,Research Article ,Cell biology ,MAP Kinase Signaling System ,NF-E2-Related Factor 2 ,Science ,03 medical and health sciences ,Drug Therapy ,Cell Line, Tumor ,Mental Health and Psychiatry ,DNA-binding proteins ,Genetics ,medicine ,Extracellular ,Humans ,Gene Regulation ,Flavonoids ,Pharmacology ,Reactive oxygen species ,Amyloid beta-Peptides ,Staining and Labeling ,Biology and life sciences ,Proteins ,Hydrogen Peroxide ,Regulatory Proteins ,Research and analysis methods ,030104 developmental biology ,Biochemical analysis ,Biophysics ,Dementia ,Neuroprotectives ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
Among various neurodegenerative diseases (NDDs), Alzheimer’s disease (AD) is the most common form. Excessive aggregation of β-amyloid protein (Aβ) is thought to be a major cause in the pathogenesis of AD. A growing number of studies also have suggested that reactive oxygen species (ROS) play a critical role in the onset and progression of AD. The present study was aimed to investigate the neuroprotective effect of the high frequency and low intensity pulsed electric field (H-LIPEF) against hydrogen peroxide (H 2 O 2 ) and Aβ-induced cytotoxicity in SH-SY5Y cells. The systematic study on frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF) was performed, and the result showed that H-LIPEF at 200 Hz conferred the best protective effect to the SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Given the fact that electric field can be modified to focus on specific area and applicable in a non-contact manner, we propose that H-LIPEF holds great potential for neuroprotection and curing the patients with NDDs. Furthermore, it is anticipated that the combination of such physical stimulation with drugs or natural compounds will be even more effective in treating NDDs.
- Published
- 2021