1. MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB
- Author
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Lili Lin, Yongsheng Hou, Nannan Yu, Jingjing Liu, and Yanyan Zhang
- Subjects
Gene Expression ,Apoptosis ,Cell Communication ,Retinal Pigment Epithelium ,Biochemistry ,Medical Conditions ,Medicine and Health Sciences ,3' Untranslated Regions ,Immune Response ,Cell Analysis ,Gene knockdown ,Multidisciplinary ,medicine.diagnostic_test ,Cell Death ,Chemistry ,Organic Compounds ,Monosaccharides ,Nucleic acids ,Real-time polymerase chain reaction ,Bioassays and Physiological Analysis ,Cell Processes ,Physical Sciences ,Medicine ,Hyperexpression Techniques ,Research Article ,Glia Maturation Factor ,Cell Viability Testing ,Cell Survival ,Science ,Immunology ,Carbohydrates ,Glia maturation factor ,Transfection ,Research and Analysis Methods ,Flow cytometry ,Cell Line ,Signs and Symptoms ,Phagocytosis ,microRNA ,medicine ,Gene Expression and Vector Techniques ,Genetics ,Humans ,Secretion ,Viability assay ,Molecular Biology Techniques ,Non-coding RNA ,Molecular Biology ,Inflammation ,Molecular Biology Assays and Analysis Techniques ,Natural antisense transcripts ,Diabetic Retinopathy ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Cell Biology ,Molecular biology ,Gene regulation ,MicroRNAs ,Glucose ,Gene Expression Regulation ,Hyperglycemia ,Metabolic Disorders ,RNA ,Clinical Medicine - Abstract
Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3’UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.
- Published
- 2021