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Your search keyword '"Kohki Yoshimoto"' showing total 2 results
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2 results on '"Kohki Yoshimoto"'

1. Thaumatin-like proteins and a cysteine protease inhibitor secreted by the pine wood nematode Bursaphelenchus xylophilus induce cell death in Nicotiana benthamiana.

Author

Haru Kirino, Kohki Yoshimoto, and Ryoji Shinya

Subjects
Medicine, Science
Abstract

Pine wilt disease (PWD) is an infectious disease of pines that typically kills affected trees. The causal pathogen of PWD is the pine wood nematode (PWN), Bursaphelenchus xylophilus. Understanding of the disease has advanced in recent years through the use of a highly sensitive proteomics procedure and whole genome sequence analysis; in combination, these approaches have enabled identification of proteins secreted by PWNs. However, the roles of these proteins during the onset of parasitism have not yet been elucidated. In this study, we used a leaf-disk assay based on transient overexpression in Nicotiana benthamiana to allow functional screening of 10 candidate pathogenic proteins secreted by PWNs. These proteins were selected based on previous secretome and RNA-seq analyses. We found that five molecules induced significant cell death in tobacco plants relative to a GFP-only control. Three of these proteins (Bx-TH1, Bx-TH2, and Bx-CPI) may have a role in molecular mimicry and likely make important contributions to inducing hypersensitive responses in host plants.

Published
2020
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2. Thaumatin-like proteins and a cysteine protease inhibitor secreted by the pine wood nematode Bursaphelenchus xylophilus induce cell death in Nicotiana benthamiana

Author

Ryoji Shinya, Haru Kirino, and Kohki Yoshimoto

Subjects
0106 biological sciences, 0301 basic medicine, Tylenchida, Science, Nicotiana benthamiana, Bursaphelenchus xylophilus, Cysteine Proteinase Inhibitors, Proteomics, 01 natural sciences, Microbiology, Host-Parasite Interactions, 03 medical and health sciences, Tobacco, Animals, Pathogen, Wilt disease, Nicotiana, Plant Diseases, Multidisciplinary, biology, Cell Death, fungi, food and beverages, Helminth Proteins, biology.organism_classification, Cysteine protease, 030104 developmental biology, Thaumatin, Medicine, 010606 plant biology & botany, Research Article
Abstract

Pine wilt disease (PWD) is an infectious disease of pines that typically kills affected trees. The causal pathogen of PWD is the pine wood nematode (PWN), Bursaphelenchus xylophilus. Understanding of the disease has advanced in recent years through the use of a highly sensitive proteomics procedure and whole genome sequence analysis; in combination, these approaches have enabled identification of proteins secreted by PWNs. However, the roles of these proteins during the onset of parasitism have not yet been elucidated. In this study, we used a leaf-disk assay based on transient overexpression in Nicotiana benthamiana to allow functional screening of 10 candidate pathogenic proteins secreted by PWNs. These proteins were selected based on previous secretome and RNA-seq analyses. We found that five molecules induced significant cell death in tobacco plants relative to a GFP-only control. Three of these proteins (Bx-TH1, Bx-TH2, and Bx-CPI) may have a role in molecular mimicry and likely make important contributions to inducing hypersensitive responses in host plants.

Published
2020

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