1. A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma
- Author
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Pierre Lescuyer, Laure Allard, Jean-Charles Sanchez, Kit-Yi Leung, Malcolm Ward, Helen Byers, and James Campbell
- Subjects
Proteomics ,Time Factors ,Proteome ,Peptide ,Proprietary software ,Peptides/chemistry ,Biochemistry ,Peptide Mapping ,Mass Spectrometry ,Mascot ,Isotopes ,Animals ,Humans ,ddc:576 ,Databases, Protein ,Molecular Biology ,Mass Spectrometry/methods ,Acute stroke ,chemistry.chemical_classification ,Ions ,Chromatography ,Computational Biology ,Proteins ,Blood Proteins ,Computational Biology/methods ,Isotope-coded affinity tag ,Up-Regulation ,chemistry ,Blood Proteins/chemistry ,Immunoglobulin G/chemistry ,Potential biomarkers ,Immunoglobulin G ,Biological Markers ,Proteomics/methods ,Peptides ,Algorithms ,Biomarkers ,Software ,Proteins/chemistry - Abstract
A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers.
- Published
- 2005