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Your search keyword '"Lo D"' showing total 16 results
Start Over Author "Lo D" Journal review of scientific instruments
16 results on '"Lo D"'

4. PIXE x rays: From Z=4 to Z=92

Author

Li, C. K., primary, Wenzel, K. W., additional, Petrasso, R. D., additional, Lo, D. H., additional, Coleman, J. W., additional, Lierzer, J. R., additional, Hsieh, E., additional, and Bernat, T., additional

Published
1992
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5. A fusion‐product source

Author

Wenzel, K. W., primary, Lo, D. H., additional, Petrasso, R. D., additional, Coleman, J. W., additional, Li, C. K., additional, Lierzer, J. R., additional, Borrás, C., additional, Wei, T., additional, Hsieh, E., additional, and Bernat, T., additional

Published
1992
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7. MIT fusion γ-ray diagnostic development

Author

Wenzel, K. W., primary, Petrasso, R. D., additional, Lo, D. H., additional, Li, C. K., additional, Coleman, J. W., additional, Lierzer, J. R., additional, Hsieh, E., additional, and Bernat, T., additional

Published
1992
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16. Novel in situ normal streaming potential device for characterizing electrostatic properties of confluent cells.

Author

Vandrangi, P., Jreij, P., Rajapaksa, T. E., Bansal, N., Lo, D. D., and Rodgers, V. G. J.

Subjects
ELECTROSTATICS, CELL membranes, TIGHT junctions, ZETA potential, ELECTRIC action of points, ELECTROTHERAPEUTICS, CELL junctions
Abstract

The characteristics of transport across confluent cell monolayers may often be attributed to its electrostatic properties. While tangential streaming potential is often used to quantify these electrostatic properties, this method is not effective for transport normal to the apical cell surface where the charge properties along the basolateral sides may be important (i.e., confluent cells with leaky tight junctions). In addition, even when cells have a uniform charge distribution, the shear stress generated by the conventional tangential flow device may dislodge cells from their confluent state. Here we introduce a novel streaming potential measurement device to characterize the normal electrostatic properties of confluent cells. The streaming potential device encompasses a 24 mm cell-seeded Transwell® with two AgCl electrodes on either side of the cell-seeded Transwell. Phosphate buffered saline is pressurized transversal to the Transwell and the resultant pressure gradient induces a potential difference. Confluent monolayers of HEK and EA926 cells are used as examples. The corresponding zeta potential of the cell-membrane configuration is calculated using the Helmholtz-Smoluchowski equation and the zeta potential of the confluent cell layer is deconvolved from the overall measurements. For these test models, the zeta potential is consistent with that determined using a commercial dispersed-cell device. This novel streaming potential device provides a simple, easy, and cost-effective methodology to determine the normal zeta potential of confluent cells cultured on Transwell systems while keeping the cells intact. Furthermore, its versatility allows periodic measurements of properties of the same cell culture during transient studies. [ABSTRACT FROM AUTHOR]

Published
2012
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